Difference between revisions of "Previous iGEM projects"
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[http://parts.mit.edu/igem07/index.php/Davidson_Missouri_W Davidson & MWSU 2007] Hin/Hix random flipping | [http://parts.mit.edu/igem07/index.php/Davidson_Missouri_W Davidson & MWSU 2007] Hin/Hix random flipping | ||
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| + | Found a small snippet from the iGEM Idea Exchange in which the MIT Synthetic Biology Working Group listed desired parts that weren't in the registry as of 2008. [http://openwetware.org/wiki/IGEM:Idea_exchange Idea Exchange] | ||
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| + | " Random Number Generator | ||
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| + | * FimE inverts a specific stretch of DNA, defined by a pair of sequence elements (IRR and IRL), forming a DNA loop between the two elements[3]. If we add multiple copies of one | ||
| + | of these elements (one IRR, two IRL), would FimE randomly choose one of the sites (one IRL out of the pair) to invert between? Either choose one of several promoters to attach | ||
| + | to a given gene, or one of several genes to attach to a given promoter. | ||
| + | * Then, can we tune the probability (from, say, 60:40 to 80:20 to 20:80)? Ideally do this dynamically (based on some small molecule) - use proteins that bend DNA to affect the | ||
| + | probability of loop formation. " | ||
Revision as of 04:11, 15 April 2010
This team tried to create a randomizer that chooses between two overlapping promoters that are mutually exclusive. One produces RFP while the other produces GFP. The team has a simulation and submitted parts to the registry but we were unable to find any conclusive results.
Davidson & MWSU 2007 Hin/Hix random flipping
Found a small snippet from the iGEM Idea Exchange in which the MIT Synthetic Biology Working Group listed desired parts that weren't in the registry as of 2008. Idea Exchange
" Random Number Generator
* FimE inverts a specific stretch of DNA, defined by a pair of sequence elements (IRR and IRL), forming a DNA loop between the two elements[3]. If we add multiple copies of one
of these elements (one IRR, two IRL), would FimE randomly choose one of the sites (one IRL out of the pair) to invert between? Either choose one of several promoters to attach
to a given gene, or one of several genes to attach to a given promoter.
* Then, can we tune the probability (from, say, 60:40 to 80:20 to 20:80)? Ideally do this dynamically (based on some small molecule) - use proteins that bend DNA to affect the
probability of loop formation. "
