Difference between revisions of "BioBrick Ligations"
From GcatWiki
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o 2 µL of SpeI or PstI | o 2 µL of SpeI or PstI | ||
o A volume of dH2O to make a total volume of 40 µL | o A volume of dH2O to make a total volume of 40 µL | ||
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| + | [[Image:biobrick_ligations.jpg]] | ||
Revision as of 17:31, 21 June 2010
• For a 40 µL Digestion o 2000 ng of DNA in an appropriate volume o 4 µL of BSA (10x) o 4 µL of NEB 2 (10X Buffer) o 2 µL of EcoRI or XbaI o 2 µL of SpeI or PstI o A volume of dH2O to make a total volume of 40 µL
