Difference between revisions of "TBLASTn and Protein Sequence Analysis"
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Occasionally BLASTing genes with just a nucleotide sequence doesn't work. This can be due to a number of reasons: | Occasionally BLASTing genes with just a nucleotide sequence doesn't work. This can be due to a number of reasons: | ||
| − | + | *Lack of conservation between species | |
| − | + | *Incomplete genome database | |
| + | *Rearrangement of introns/exons | ||
| + | |||
| + | If, for any of these reasons, you aren't getting any hits, it might be time to make sure you're doing it right: | ||
| + | |||
| + | First - | ||
== Beyond blastn == | == Beyond blastn == | ||
== Why Amino Acids? == | == Why Amino Acids? == | ||
Revision as of 18:34, 22 February 2011
When Nucleotides Don't Work
Occasionally BLASTing genes with just a nucleotide sequence doesn't work. This can be due to a number of reasons:
- Lack of conservation between species
- Incomplete genome database
- Rearrangement of introns/exons
If, for any of these reasons, you aren't getting any hits, it might be time to make sure you're doing it right:
First -
