Difference between revisions of "Malcolm C"
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The class has already completed 4 SSR primer designs (LDOX, CHS, StSy, and F3H). | The class has already completed 4 SSR primer designs (LDOX, CHS, StSy, and F3H). | ||
| − | Results from Blueberry 454 Scaffold Searches from Grape (given to | + | Results from Blueberry 454 Scaffold Searches from Grape (given to us by Allan Brown)<br> |
'''AOMT Scaffold 00796''' | '''AOMT Scaffold 00796''' | ||
# Forward Primer GAGCTCTTGGATGCTCCATACT & Rev Primer CCTCATAATTTTTGGTCTTCGC (taa) x6 PCR product = 137 bp | # Forward Primer GAGCTCTTGGATGCTCCATACT & Rev Primer CCTCATAATTTTTGGTCTTCGC (taa) x6 PCR product = 137 bp | ||
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| − | CHI scaffold03186 | + | '''CHI scaffold03186''' |
| + | # Forward Primer TTTCTATCTCTCTCGCCGTCAT & Rev Primer GTTTTCTCACCTTGTTTTTGCC (ct) x21 PCR product = 219 bp | ||
| + | # Forward Primer CCGTTTCGATCTCTCTCTCAGT & Rev Primer CATCCCTAGATCTGACACCACA (tc) x14 PCR product = 278 bp | ||
| + | |||
| − | DFR scaffold 03132 | + | '''DFR scaffold 03132''' |
| − | F3'H scaffold 01438 | + | '''F3'H scaffold 01438''' |
| − | FLS scaffold 00142 | + | '''FLS scaffold 00142''' |
| − | FLS scaffold 00374 | + | '''FLS scaffold 00374''' |
| − | MYBA1 scaffold 03500 | + | '''MYBA1 scaffold 03500''' |
| − | MYCA1 scaffold 00278 | + | '''MYCA1 scaffold 00278''' |
| − | MYCA1 scaffold 00554 | + | '''MYCA1 scaffold 00554''' |
| − | UFGT scaffold 00304 | + | '''UFGT scaffold 00304''' |
| − | UFGT scaffold 01120 | + | '''UFGT scaffold 01120''' |
Revision as of 21:01, 28 February 2012
This is where I will capture my progress on this project.
I will also be working on the 13 genes Allan gave us.
The class has already completed 4 SSR primer designs (LDOX, CHS, StSy, and F3H).
Results from Blueberry 454 Scaffold Searches from Grape (given to us by Allan Brown)
AOMT Scaffold 00796
- Forward Primer GAGCTCTTGGATGCTCCATACT & Rev Primer CCTCATAATTTTTGGTCTTCGC (taa) x6 PCR product = 137 bp
- Forward Primer TATCCCATGTTTTGGCTAATCC & Rev Primer TTTGCTTTTCAATCGGGAAC (tc) x9 PCR product = 261 bp
AOMT scaffold 01781
- Forward Primer GGCGACTGTGTCTTTCAGTAAA & Rev Primer TAGGATTTCGAGGAGGAGAGGT (ct) x7 PCR product = 136 bp
- Forward Primer ATAAACAGGTAAACCGAACCGA & Rev Primer CCGATTAAATTGGAGCAGTAGC (tg) x7 PCR product = 262 bp
ANPER scaffold 00666
- Forward Primer TTTCTATCTCTCTCGCCGTCAT & Rev Primer GTTTTCTCACCTTGTTTTTGCC (ct) x21 PCR product = 219 bp
- Forward Primer CCGTTTCGATCTCTCTCTCAGT & Rev Primer CATCCCTAGATCTGACACCACA (tc) x14 PCR product = 278 bp
CHI scaffold03186
- Forward Primer TTTCTATCTCTCTCGCCGTCAT & Rev Primer GTTTTCTCACCTTGTTTTTGCC (ct) x21 PCR product = 219 bp
- Forward Primer CCGTTTCGATCTCTCTCTCAGT & Rev Primer CATCCCTAGATCTGACACCACA (tc) x14 PCR product = 278 bp
DFR scaffold 03132
F3'H scaffold 01438
FLS scaffold 00142
FLS scaffold 00374
MYBA1 scaffold 03500
MYCA1 scaffold 00278
MYCA1 scaffold 00554
UFGT scaffold 00304
UFGT scaffold 01120
