Difference between revisions of "Malcolm C"
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# Forward Primer TACATCTTGAGGGGCAGTTTTT & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp | # Forward Primer TACATCTTGAGGGGCAGTTTTT & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp | ||
# Forward Primer ACGTGGTCCAATCTAACCTCAC & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp | # Forward Primer ACGTGGTCCAATCTAACCTCAC & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp | ||
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'''F3'H scaffold 01438''' | '''F3'H scaffold 01438''' | ||
# Forward Primer CAGTGGTACAGTGGTGGATGAC & Rev Primer ATTCCCTTTCTCTTCCCATTTC (ag) x7 PCR product = 152 bp | # Forward Primer CAGTGGTACAGTGGTGGATGAC & Rev Primer ATTCCCTTTCTCTTCCCATTTC (ag) x7 PCR product = 152 bp | ||
# Forward Primer ATACCAATCCATAGTCTCCCGA & Rev Primer TCAGTTGTGTTTTTCAGTTGGG (ta) x7 PCR product = 279 bp | # Forward Primer ATACCAATCCATAGTCTCCCGA & Rev Primer TCAGTTGTGTTTTTCAGTTGGG (ta) x7 PCR product = 279 bp | ||
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'''FLS scaffold 00142''' | '''FLS scaffold 00142''' | ||
| + | # Forward Primer CAATTATACCCTTCCTGTCCCA & Rev Primer GAATGGATGAAAGGAACCCATA (ag) x8 PCR product = 214 bp | ||
| + | # Forward Primer CTAGCTCTCCCTCTCTCCCTCT & Rev Primer GTGGTACTCCATATCCATGCCT (ct) x8 PCR product = 295 bp | ||
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'''FLS scaffold 00374''' | '''FLS scaffold 00374''' | ||
Revision as of 21:12, 28 February 2012
This is where I will capture my progress on this project.
I will also be working on the 13 genes Allan gave us.
The class has already completed 4 SSR primer designs (LDOX, CHS, StSy, and F3H).
Results from Blueberry 454 Scaffold Searches from Grape (given to us by Allan Brown)
AOMT Scaffold 00796
- Forward Primer GAGCTCTTGGATGCTCCATACT & Rev Primer CCTCATAATTTTTGGTCTTCGC (taa) x6 PCR product = 137 bp
- Forward Primer TATCCCATGTTTTGGCTAATCC & Rev Primer TTTGCTTTTCAATCGGGAAC (tc) x9 PCR product = 261 bp
AOMT scaffold 01781
- Forward Primer GGCGACTGTGTCTTTCAGTAAA & Rev Primer TAGGATTTCGAGGAGGAGAGGT (ct) x7 PCR product = 136 bp
- Forward Primer ATAAACAGGTAAACCGAACCGA & Rev Primer CCGATTAAATTGGAGCAGTAGC (tg) x7 PCR product = 262 bp
ANPER scaffold 00666
- Forward Primer TTTCTATCTCTCTCGCCGTCAT & Rev Primer GTTTTCTCACCTTGTTTTTGCC (ct) x21 PCR product = 219 bp
- Forward Primer CCGTTTCGATCTCTCTCTCAGT & Rev Primer CATCCCTAGATCTGACACCACA (tc) x14 PCR product = 278 bp
CHI scaffold03186
- Forward Primer TACATCTTGAGGGGCAGTTTTT & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
- Forward Primer ACGTGGTCCAATCTAACCTCAC & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp
DFR scaffold 03132
- Forward Primer TACATCTTGAGGGGCAGTTTTT & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
- Forward Primer ACGTGGTCCAATCTAACCTCAC & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp
F3'H scaffold 01438
- Forward Primer CAGTGGTACAGTGGTGGATGAC & Rev Primer ATTCCCTTTCTCTTCCCATTTC (ag) x7 PCR product = 152 bp
- Forward Primer ATACCAATCCATAGTCTCCCGA & Rev Primer TCAGTTGTGTTTTTCAGTTGGG (ta) x7 PCR product = 279 bp
FLS scaffold 00142
- Forward Primer CAATTATACCCTTCCTGTCCCA & Rev Primer GAATGGATGAAAGGAACCCATA (ag) x8 PCR product = 214 bp
- Forward Primer CTAGCTCTCCCTCTCTCCCTCT & Rev Primer GTGGTACTCCATATCCATGCCT (ct) x8 PCR product = 295 bp
FLS scaffold 00374
MYBA1 scaffold 03500
MYCA1 scaffold 00278
MYCA1 scaffold 00554
UFGT scaffold 00304
UFGT scaffold 01120
