Difference between revisions of "File:ThyA on Amp plates.jpg"
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(We took the transformed cells with the thyA fitness module and plated them on just Ampicillin plates. This was to mitigate having to select for both kanamycin and ampicillin resistance. Only transformed cells were expected to grow and they did in compa...) |
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| − | We took the transformed cells with the thyA fitness module and plated them on just Ampicillin plates. This was to mitigate having to select for both kanamycin and ampicillin resistance. Only transformed cells were expected to grow and they did in comparison with the negative control plated on amp. The negative control is just the plasmid that carries the kanamycin resistance, so it would have needed kanamycin to grow. | + | We took the transformed cells with the thyA fitness module and plated them on just Ampicillin plates. This was to mitigate having to select for both kanamycin and ampicillin resistance. Only transformed cells were expected to grow and they did in comparison with the negative control plated on amp. The negative control is just the plasmid that carries the kanamycin resistance, so it would have needed kanamycin to grow. Negative control on top. Experimental on the bottom. |
Revision as of 18:19, 3 June 2014
We took the transformed cells with the thyA fitness module and plated them on just Ampicillin plates. This was to mitigate having to select for both kanamycin and ampicillin resistance. Only transformed cells were expected to grow and they did in comparison with the negative control plated on amp. The negative control is just the plasmid that carries the kanamycin resistance, so it would have needed kanamycin to grow. Negative control on top. Experimental on the bottom.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 18:13, 3 June 2014 |  | 311 × 543 (18 KB) | Tetaye (talk | contribs) | We took the transformed cells with the thyA fitness module and plated them on just Ampicillin plates. This was to mitigate having to select for both kanamycin and ampicillin resistance. Only transformed cells were expected to grow and they did in compa... |
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