Difference between revisions of "Repeating Eckdahl's 20-clone selection"
Macampbell (talk | contribs) (Created page with "We need to replicate Dr. Eckdahl’s 20-clone experiment twice on both campuses and mathematically analyze the data. Continue adding arabinose to induce the chaperones. We ne...") |
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Depending on the survivorship of the clones in the previous step, set up plates to run head-to-head competition between two surviving clones, and determine which strain is most competent. This will confirm the results of the 20-clone experiment, in a more controlled environment than a plate with all 20 clones. | Depending on the survivorship of the clones in the previous step, set up plates to run head-to-head competition between two surviving clones, and determine which strain is most competent. This will confirm the results of the 20-clone experiment, in a more controlled environment than a plate with all 20 clones. | ||
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| + | Protocol created on 05/21/14: [[Eckdahl-replication-protocol]] | ||
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| + | [https://www.dropbox.com/sh/2jqkqfz755ng0vc/AAA26M9kEeYii0pFtFlCQ17na Photos from Davidson's first replication (four plates)] (Updated May 31, 2014) | ||
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| + | Template for results: [[File:Replication Results Template.xlsx]] | ||
| + | Upload a file for each campus with the results displayed in the table. There is a tab in the file for each trial. | ||
Latest revision as of 18:38, 4 June 2014
We need to replicate Dr. Eckdahl’s 20-clone experiment twice on both campuses and mathematically analyze the data. Continue adding arabinose to induce the chaperones.
We need to grow the surviving clones on individual plates: no antibiotic, amp, amp+chlor. This will enable us to determine whether both plasmids (CDM+amp, chaperone+chlor) are still present and being transcribed.
Depending on the survivorship of the clones in the previous step, set up plates to run head-to-head competition between two surviving clones, and determine which strain is most competent. This will confirm the results of the 20-clone experiment, in a more controlled environment than a plate with all 20 clones.
Protocol created on 05/21/14: Eckdahl-replication-protocol
Photos from Davidson's first replication (four plates) (Updated May 31, 2014)
Template for results: File:Replication Results Template.xlsx Upload a file for each campus with the results displayed in the table. There is a tab in the file for each trial.
