Difference between revisions of "JP Jan 28 16"
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| + | 72 nucleotide reads. What gene do they go to? How many of each are there? | ||
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| + | How to count number of genes and compare across time points? Normalize to a housekeeping gene. OR standard procedure (look up). | ||
| + | * "How to normalize gene expression given you don't get the same number of reads in each sample" | ||
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| + | How to know we have the right tissue? | ||
| + | * Blast overrepresented sequences of trimmed intestinal sequences. | ||
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| + | Have asked for a list of the genes expressed in each organ from the previous paper. (Todd?) | ||
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| + | * How can we use the data repository, can we query? SRP051827 | ||
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| + | * Two teams to work in pairs to figure out a way to take the text file and add a number to "protein of unknown function" / see if other genes are named more than once. | ||
Latest revision as of 18:50, 28 January 2016
72 nucleotide reads. What gene do they go to? How many of each are there?
How to count number of genes and compare across time points? Normalize to a housekeeping gene. OR standard procedure (look up).
- "How to normalize gene expression given you don't get the same number of reads in each sample"
How to know we have the right tissue?
- Blast overrepresented sequences of trimmed intestinal sequences.
Have asked for a list of the genes expressed in each organ from the previous paper. (Todd?)
- How can we use the data repository, can we query? SRP051827
- Two teams to work in pairs to figure out a way to take the text file and add a number to "protein of unknown function" / see if other genes are named more than once.
