Difference between revisions of "Q5 PCR NEB"
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Revision as of 20:46, 14 June 2016
Reaction Setup
REAGENT | VOLUME (µL) | FINAL CONC. |
---|---|---|
10 µM Forward Primer | 2.5 µL | 0.5 µM |
10 µM Reverse Primer | 2.5 µL | 0.5 µM |
Template DNA | Y (1ng) | 1 ng |
Q5 High-Fidelity 2X Master Mix | 25 µL | 1X |
Nuclease-free Water | 20- Y |
Final Volume = 50µL
Notes:
• Q5 High-Fidelity 2X Master Mix has an error rate > 100-fold lower than that of Taq DNA Polymerase
• Q5 High-Fidelity 2X Master Mix can be stored at -20°C. If precipitate forms after thawing, resuspend before use
• Remember to first dilute primers in nuclease-free water if starting with a higher concentration
• Do not exceed 50 µL final volume