Difference between revisions of "Nanodrop DNA for Bio113"

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(Created page with " == Nanodrop DNA == # The Nanodrop will be blanked using the elution buffer from your mini prep. # Pipet 2 µL of your mini prep onto the small open stop on the metal circle....")
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Revision as of 21:12, 18 July 2017

Nanodrop DNA

  1. The Nanodrop will be blanked using the elution buffer from your mini prep.
  2. Pipet 2 µL of your mini prep onto the small open stop on the metal circle.
  3. Carefully lower the arm and click on the measure button.
  4. Record these values for each of your samples:
  • value at 260 nm
  • concentration of your DNA in ng/µL
  • 26/280 ratio value