Difference between revisions of "Nanodrop DNA for Bio113"
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Macampbell (talk | contribs) (Created page with " == Nanodrop DNA == # The Nanodrop will be blanked using the elution buffer from your mini prep. # Pipet 2 µL of your mini prep onto the small open stop on the metal circle....") |
Macampbell (talk | contribs) |
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# Carefully lower the arm and click on the measure button. | # Carefully lower the arm and click on the measure button. | ||
# Record these values for each of your samples: | # Record these values for each of your samples: | ||
| − | * value at 260 nm | + | * absorbance value of your DNA at 260 nm |
* concentration of your DNA in ng/µL | * concentration of your DNA in ng/µL | ||
* 26/280 ratio value | * 26/280 ratio value | ||
Revision as of 21:13, 18 July 2017
Nanodrop DNA
- The Nanodrop will be blanked using the elution buffer from your mini prep.
- Pipet 2 µL of your mini prep onto the small open stop on the metal circle.
- Carefully lower the arm and click on the measure button.
- Record these values for each of your samples:
- absorbance value of your DNA at 260 nm
- concentration of your DNA in ng/µL
- 26/280 ratio value
