Difference between revisions of "Nanodrop DNA for Bio113"
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* absorbance value of your DNA at 260 nm | * absorbance value of your DNA at 260 nm | ||
* concentration of your DNA in ng/µL | * concentration of your DNA in ng/µL | ||
| − | * | + | * 260/280 ratio value |
Revision as of 19:45, 9 November 2017
Nanodrop DNA
- The Nanodrop will be blanked using the elution buffer from your mini prep.
- Pipet 2 µL of your mini prep onto the small open stop on the metal circle.
- Carefully lower the arm and click on the measure button.
- Record these values for each of your samples:
- absorbance value of your DNA at 260 nm
- concentration of your DNA in ng/µL
- 260/280 ratio value
