Difference between revisions of "Nanodrop DNA for Bio113"
From GcatWiki
Macampbell (talk | contribs) |
Macampbell (talk | contribs) |
||
| Line 5: | Line 5: | ||
# Carefully lower the arm and click on the measure button. | # Carefully lower the arm and click on the measure button. | ||
# Record these values for each of your samples: | # Record these values for each of your samples: | ||
| − | * absorbance value of your DNA at 260 nm | + | ** absorbance value of your DNA at 260 nm |
* concentration of your DNA in ng/µL | * concentration of your DNA in ng/µL | ||
* 260/280 ratio value | * 260/280 ratio value | ||
Revision as of 15:29, 3 January 2020
Nanodrop DNA
- The Nanodrop will be blanked using the elution buffer from your mini prep.
- Pipet 2 µL of your mini prep onto the small open stop on the metal circle.
- Carefully lower the arm and click on the measure button.
- Record these values for each of your samples:
- absorbance value of your DNA at 260 nm
- concentration of your DNA in ng/µL
- 260/280 ratio value
