Difference between revisions of "Divide and Conquer Biological Challenges"
From GcatWiki
MaCampbell (talk | contribs) (→* We need to get the Lsr/AI-2 system working.) |
MaCampbell (talk | contribs) (→* We need to get the Lsr/AI-2 system working.) |
||
| Line 4: | Line 4: | ||
=== * We need to get the Lsr/AI-2 system working. === | === * We need to get the Lsr/AI-2 system working. === | ||
Here are '''NO''' Lsr parts in the registry: <br> | Here are '''NO''' Lsr parts in the registry: <br> | ||
| + | [[Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system]] | ||
We will need to make BB parts for:<br> | We will need to make BB parts for:<br> | ||
| − | Lsr | + | LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp. |
| + | Lsr promoter | ||
| + | LuxS that produces DPD that somehow is converted to ''R''-THMF. | ||
| + | How do we make AI-2? | ||
=== * We do NOT need to use additional chemical input signals (''e.g.'', aTc and IPTG). We will use only AHL and AI-2 added exogenously. === | === * We do NOT need to use additional chemical input signals (''e.g.'', aTc and IPTG). We will use only AHL and AI-2 added exogenously. === | ||
Revision as of 21:02, 28 May 2008
Contents
- 1 * We need to get the Lux/AHL system working.
- 2 * We need to get the Lsr/AI-2 system working.
- 3 * We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
- 4 * We need to have an XOR logic gate produced.
- 5 * We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
* We need to get the Lux/AHL system working.
* We need to get the Lsr/AI-2 system working.
Here are NO Lsr parts in the registry:
Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system
We will need to make BB parts for:
LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp.
Lsr promoter
LuxS that produces DPD that somehow is converted to R-THMF.
How do we make AI-2?
* We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
* We need to have an XOR logic gate produced.
Existing Logic Gates
- NOT: (insert links to examples here)
- AND: (insert links to examples here)
* We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
What if AmpR is secreted and cells are not AmpR? This would prevent cells down the chain from responding too soon.
