Difference between revisions of "Divide and Conquer Biological Challenges"
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MaCampbell (talk | contribs) (→* We are interested in building the [http://partsregistry.org/Part:BBa_K091100 pLac_lux promoter].) |
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Revision as of 14:01, 2 June 2008
Contents
- 1 * We are interested in building the pLac_lux promoter.
- 2 * We need to get the Lux/AHL system working.
- 3 * We might need to get the Lsr/AI-2 system working.
- 4 * We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
- 5 * We need to have an XOR logic gate produced.
- 6 * We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
* We are interested in building the pLac_lux promoter.
If LuxR and LacIQ are always on.....when you have IPTG AND NOT AHL
* We need to get the Lux/AHL system working.
* We might need to get the Lsr/AI-2 system working.
Our summary of the Lsr system: Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system
- There are NO Lsr parts in the registry:
- We will need to make BB parts for:
- LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp.
- Lsr promoter
- LuxS that produces DPD that somehow is converted to R-THMF.
- How do we get cells to make AI-2?
* We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
* We need to have an XOR logic gate produced.
Existing Logic Gates
- AND:
Existing But Complex AND
Simple But Hypothetical AND
We would need to have:
- Starts with pTetR
- over production of LacIQ repressor but note this link is plain LacI
- over production of TetR repressor
- Therefore, we will have to build 1) modified promoter and 2) LacIQ
- NAND:
- OR:
Simple but Hypothetical OR
- XOR:
See ETH's Designed but not build XOR gate. They also published this here
Also, there is a tunable quarum sensor here.
Simple but Hypothetical XOR
- NOR:
- NOT (Inverters):
Self-Contained Inverters
PoPS Output Inverters
* We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
- What if AmpR is secreted and cells are not AmpR?
This would prevent cells down the chain from responding too soon. Erin and Pallavi have conducted experiments with this for both distance and timing.
