Difference between revisions of "Resuspending Oligos"
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| Line 3: | Line 3: | ||
Dilute 100X to 100ng/ul | Dilute 100X to 100ng/ul | ||
| + | |||
| + | use 1 ul in 20 ul PCR | ||
| + | B. Oligos used as primers only | ||
| + | add pure dH20 for 200 pmol/ul (e.g. 19.5 nmol= 19500 pmol; 19500 pmol/ 200 pmol/ul= 97.5 ul) | ||
| + | label as 200 pmol/ul, freeze | ||
| + | |||
| + | dilute 10X to 20 pmol/ul | ||
use 1 ul in 20 ul PCR | use 1 ul in 20 ul PCR | ||
Revision as of 19:13, 9 June 2008
A. Oligos used as both templates and primers.
add pure dH2O for 10 ug/ul (e.g. 2.10mg/210ul)
Dilute 100X to 100ng/ul
use 1 ul in 20 ul PCR
B. Oligos used as primers only
add pure dH20 for 200 pmol/ul (e.g. 19.5 nmol= 19500 pmol; 19500 pmol/ 200 pmol/ul= 97.5 ul) label as 200 pmol/ul, freeze
dilute 10X to 20 pmol/ul
use 1 ul in 20 ul PCR
