Difference between revisions of "Resuspending Oligos"
From GcatWiki
| Line 6: | Line 6: | ||
use 1 ul in 20 ul PCR | use 1 ul in 20 ul PCR | ||
B. Oligos used as primers only | B. Oligos used as primers only | ||
| − | add pure dH20 for 200 pmol/ul (e.g. 19.5 | + | add pure dH20 for 200 pmol/ul (e.g. 19.5 nmols= 19500 pmols; 19500 pmol/ 200 pmol/ul= 97.5 ul) |
label as 200 pmol/ul, freeze | label as 200 pmol/ul, freeze | ||
Latest revision as of 19:14, 9 June 2008
A. Oligos used as both templates and primers.
add pure dH2O for 10 ug/ul (e.g. 2.10mg/210ul)
Dilute 100X to 100ng/ul
use 1 ul in 20 ul PCR
B. Oligos used as primers only
add pure dH20 for 200 pmol/ul (e.g. 19.5 nmols= 19500 pmols; 19500 pmol/ 200 pmol/ul= 97.5 ul) label as 200 pmol/ul, freeze
dilute 10X to 20 pmol/ul
use 1 ul in 20 ul PCR
