Difference between revisions of "Test Mnt/Tet promoter"
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add aTc does not glow | add aTc does not glow | ||
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+ | '''06/30/08''' | ||
+ | K091105 ligated with E0240 | ||
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+ | need to ligate some promoter with an RBS and ligate TetR with a double terminator | ||
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+ | then ligate those two parts together | ||
+ | |||
+ | then ligate that part with the Mnt/Tet with GFP |
Revision as of 19:14, 30 June 2008
Test Mnt/Tet promoter
The pMnt/TetR promoter is constitutively on and is repressed by the TetR protein. The addition of aTc should repress the TetR causing it to glow. The addition of the Mnt protein should repress the promoter causing it not glow.
Test tet repression
Some promoter (pbad)→RBS+TetR+t+t+pMnt/Tet→RBS+GFP does not glows
I13453+RBS+C0040+TT+K091105+E0240
add aTc
Some promoter→TetR+t+t+pMnt/Tet→GFP glows
I13453+RBS+C0040+TT+K091105+E0240
Test Mnt repression:
Some promoter→Mnt+t+t+pMnt/Tet→GFP glows
I13453+RBS+C0072+TT+K091105+E0240
Test Tet and Mnt repression:
Some promoter→TetR+Mnt+t+t+pMnt/Tet→GFP does not glows
I13453+RBS+C0040+C0072+TT+K091105+E0240
add aTc does not glow
06/30/08
K091105 ligated with E0240
need to ligate some promoter with an RBS and ligate TetR with a double terminator
then ligate those two parts together
then ligate that part with the Mnt/Tet with GFP