Difference between revisions of "Test pLsrR/cI"
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Revision as of 19:51, 30 June 2008
pLsrR/cI is constitutively on and is repressed by LsrR. The addition of AI-2, which becomes phosphorylated by LsrK, represses LsrR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.
Some promoter→LsrK+LsrR+t+t+pLsrR/cI→GFP does not glow
add AI-2
Some promoter→LsrK+LsrR+t+t+pLsrR/cI→GFP glows
add AI-2
Some promoter→LsrK+LsrR+cI+t+t+pLsrR/cI→GFP does not glows
| LsrR | cI | AI-2 + GFP | AI-2 - GFP |
|---|---|---|---|
| 0 | 0 | 1 | 1 |
| 1 | 0 | 1 | 0 |
| 0 | 1 | 0 | 0 |
| 1 | 1 | 0 | 0 |
