Difference between revisions of "Test pLsrR/cI"

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pLsrR/cI is constitutively on and is repressed by LsrR. The addition of AI-2, which becomes phosphorylated by LsrK, represses LsrR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.
+
pLsrA/cI is constitutively on and is repressed by LsrR. The addition of AI-2, which becomes phosphorylated by LsrK, represses LsrR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.
  
Some promoter→LsrK+LsrR+t+t+pLsrR/cI→GFP  does not glow
+
1)Test LsrA repression.
  
 +
Some promoter→RBS+LsrR+t+t+pLsrA/cI→GFP  does not glow
  
 
add AI-2               
 
add AI-2               
 
                                                  
 
                                                  
Some promoter→LsrK+LsrR+t+t+pLsrR/cI→GFP glows
+
Some promoter→RBS+LsrR+t+t+pLsrA/cI→GFP glows
  
 +
 +
2)Test cI repression.
 +
 +
Some promoter→RBS+cI+t+t+pLsrA/cI→GFP  does not glow
 +
 +
add cI
 +
 +
Some promoter→RBS+cI+t+t+pLsrA/cI→GFP  does not glow
 +
 +
 +
3)Test LsrA and cI repression.
 +
 +
Some promoter→LsrK+LsrR+cI+t+t+pLsrR/cI→GFP does not glow
  
 
add AI-2  
 
add AI-2  
 
                                                                
 
                                                                
Some promoter→LsrK+LsrR+cI+t+t+pLsrR/cI→GFP does not glows
+
Some promoter→LsrK+LsrR+cI+t+t+pLsrR/cI→GFP does not glow
 +
 
 +
4)Control.
 +
 
 +
pLsrR/cI→GFP glows
  
 
{| border="1" cellpadding="2"
 
{| border="1" cellpadding="2"
!width="30"|LsrR
+
!width="30"|LsrA
 
!width="30"|cI
 
!width="30"|cI
 
!width="30"|AI-2 + GFP
 
!width="30"|AI-2 + GFP

Revision as of 13:53, 1 July 2008

pLsrA/cI is constitutively on and is repressed by LsrR. The addition of AI-2, which becomes phosphorylated by LsrK, represses LsrR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.

1)Test LsrA repression.

Some promoter→RBS+LsrR+t+t+pLsrA/cI→GFP does not glow

add AI-2

Some promoter→RBS+LsrR+t+t+pLsrA/cI→GFP glows


2)Test cI repression.

Some promoter→RBS+cI+t+t+pLsrA/cI→GFP does not glow

add cI

Some promoter→RBS+cI+t+t+pLsrA/cI→GFP does not glow


3)Test LsrA and cI repression.

Some promoter→LsrK+LsrR+cI+t+t+pLsrR/cI→GFP does not glow

add AI-2

Some promoter→LsrK+LsrR+cI+t+t+pLsrR/cI→GFP does not glow

4)Control.

pLsrR/cI→GFP glows

LsrA cI AI-2 + GFP AI-2 - GFP
0 0 1 1
1 0 1 0
0 1 0 0
1 1 0 0