Difference between revisions of "Davidson Missouri W/Davidson Protocols"

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# [[Davidson Missouri W/Primer_dimer| Making dsDNA Using Primer Dimers]]
 
# [[Davidson Missouri W/Primer_dimer| Making dsDNA Using Primer Dimers]]
  
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'''Web Pages We Use'''
+
'''Web Tools We Use'''
 
#[http://gcat.davidson.edu/StudentProjects/mwk/gelwebsite.html Optimize your Gel]
 
#[http://gcat.davidson.edu/StudentProjects/mwk/gelwebsite.html Optimize your Gel]
 
#[http://gcat.davidson.edu/iGEM07/genesplitter.html Gene Splitting Web Site]
 
#[http://gcat.davidson.edu/iGEM07/genesplitter.html Gene Splitting Web Site]

Revision as of 15:14, 2 July 2008

  1. How to Keep a Lab Notebook
  2. Common molecular reagents
  3. Standard Assembly
  4. BioBrick Ends
  5. Compatibility of Plasmids
  6. Building dsDNA with Oligos
  7. Setting up PCR mixtures
  8. PCR and Mg2+ concentration
  9. Clean and Concentrate DNA (after PCR, before digestion)
  10. Pouring an agarose gel
  11. Calculate MWs
  12. Digest DNA with restriction enzymes
  13. Double Digest Guide
  14. Ethanol Precipitate DNA (short protocol)
  15. 1kb MW markers
  16. Shrimp Alkaline Phosphatase
  17. Qiagen QIAquick Gel Purification
  18. Qiagen QIAquick Column Regeneration Protocol
  19. ElectroElute Gel Purification
  20. Ligation Protocol
  21. Heat Shock Transformation OR Short version of Heat Shock
  22. Zippy Transformation
  23. Colony PCR to Screen for Successful Ligations
  24. Promega miniprep
  25. Choices for Transformation: Heat Shock vs. Zyppy
  26. Choices for Mini-Preps: Promega vs. Zyppy
  27. Making dsDNA Using Primer Dimers

Web Tools We Use

  1. Optimize your Gel
  2. Gene Splitting Web Site
  3. PCR Primers w/ BioBricks
  4. Promega Tm Calculator
  5. List of auto-inducers and their Sigma catalog numbers
  6. Sequencing at CUGI
  7. Lance-olator Oligos for dsDNA assembly
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