Difference between revisions of "LuxR/cI"
From GcatWiki
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| 3 || Product of 2 || RBS + LuxS + RBS || RX/SP || SP || || Product of 1A || Mnt Gene + TT || RX/SP || XP || || || | | 3 || Product of 2 || RBS + LuxS + RBS || RX/SP || SP || || Product of 1A || Mnt Gene + TT || RX/SP || XP || || || | ||
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− | | 4 || K091107 || pLux/cI || RX/SP || SP || || Product of 3 || RBS + LuxS + RBS + Mnt + TT || RX/SP || XP || || || | + | | 4 || K091107 || pLux/cI || RX/SP || SP || || Product of 3 || RBS + LuxS + RBS + Mnt + TT || RX/SP || XP || || || Objective 1 |
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Revision as of 15:58, 2 July 2008
Parallel Biobrick Assembly
Objective 1: pLux/cI + RBS + LuxS + RBS + Mnt + TT (ampicillin resistance)
K091107 + B0034 + K091109 + B0034 + C0072 + B0015[B0010+B0012]
Step | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Size |
---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Vector | . | . | . | . | Insert | . | . | . | . | Product | . |
1A | B0015 | Double Terminator | RX/SP | RX | 129bp | C0072 | Mnt Gene | RX/SP | RS | 288bp | ||
1B | B0034 | RBS | RX/SP | RX | 12bp | K091109 | LuxS Gene | RX/SP | RS | 516bp | ||
2 | B0034 | RBS | RX/SP | SP | 12bp | Product of 1B | LuxS + RBS | RX/SP | XP | |||
3 | Product of 2 | RBS + LuxS + RBS | RX/SP | SP | Product of 1A | Mnt Gene + TT | RX/SP | XP | ||||
4 | K091107 | pLux/cI | RX/SP | SP | Product of 3 | RBS + LuxS + RBS + Mnt + TT | RX/SP | XP | Objective 1 |
Objective 2: pBAD + RBS + LuxR + TT (kanamycin resistance)
Step | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Size |
---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Vector | . | . | . | . | Insert | . | . | . | . | Product | . |
1 | ||||||||||||