Difference between revisions of "LuxR/cI"
From GcatWiki
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!width="35"|Vector | !width="35"|Vector | ||
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− | |[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 | + | |[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 |
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− | |[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 | + | |[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 |
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− | |[http://partsregistry.org/Part:BBa_C0072 C0072] ||Mnt Gene ||registry 07 ||Plt.2-5M ||pSB2K3 | + | |[http://partsregistry.org/Part:BBa_C0072 C0072] ||Mnt Gene ||registry 07 ||Plt.2-5M ||pSB2K3 |
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− | |[http://partsregistry.org/Part:BBa_K091107 K091107] ||pLux/cI || N/A | + | |[http://partsregistry.org/Part:BBa_K091107 K091107] ||pLux/cI || N/A || || |
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− | |[http://partsregistry.org/Part:BBa_K091109 K091109] ||LuxS || N/A | + | |[http://partsregistry.org/Part:BBa_K091109 K091109] ||LuxS || N/A || || |
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Revision as of 16:16, 2 July 2008
Parallel Biobrick Assembly
Objective 1: pLux/cI + RBS + LuxS + RBS + Mnt + TT (ampicillin resistance)
K091107 + B0034 + K091109 + B0034 + C0072 + B0015[B0010+B0012]
Step | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Size |
---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Vector | . | . | . | . | Insert | . | . | . | . | Product | . |
1A | B0015 | Double Terminator | RX/SP | RX | 129bp | C0072 | Mnt Gene | RX/SP | RS | 288bp | ||
1B | B0034 | RBS | RX/SP | RX | 12bp | K091109 | LuxS Gene | RX/SP | RS | 516bp | ||
2 | B0034 | RBS | RX/SP | SP | 12bp | Product of 1B | LuxS + RBS | RX/SP | XP | |||
3 | Product of 2 | RBS + LuxS + RBS | RX/SP | SP | Product of 1A | Mnt Gene + TT | RX/SP | XP | ||||
4 | K091107 | pLux/cI | RX/SP | SP | Product of 3 | RBS + LuxS + RBS + Mnt + TT | RX/SP | XP | Objective 1 |
Parts
BBa_ | Description | Source | location('07) | Vector | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
B0015 | double terminator | registry 07 | Plt.1-1I;Plt.3-3O | pSB1AK3 | ||||||||
B0034 | RBS | registry 07 | Plt.1-3O | pSB1A2 | ||||||||
C0072 | Mnt Gene | registry 07 | Plt.2-5M | pSB2K3 | ||||||||
K091107 | pLux/cI | N/A | ||||||||||
K091109 | LuxS | N/A |
Step | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Size |
---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Vector | . | . | . | . | Insert | . | . | . | . | Product | . |
1 | ||||||||||||