Difference between revisions of "Parts needed for XOR"

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{| border="1" cellpadding="2"
 
{| border="1" cellpadding="2"
−
!width="50"|Part
+
!width="60"|Part
 
!width="225"|Description
 
!width="225"|Description
−
!width="225"|Student Responsible (DC or MW)
+
!width="50"|Location(07 registry) and Vector
−
!width="30"|Location(07 registry) and Vector
 
 
!width="30"|Resistance
 
!width="30"|Resistance
 
!width="30"|Results  
 
!width="30"|Results  
 
!width="30"|miniprep
 
!width="30"|miniprep
 
|-
 
|-
−
|BBa_|| || ||  ||  ||  ||
+
|[http://partsregistry.org/Part:BBa_C0051 BBa_C0051]||. cI w/ LVA  ||Plt.1-5G; pSB1A2 ||amp ||  ||
 
|-
 
|-
−
|BBa_I15030 || Lux-sender(Autoinducing)Codes for LuxI and LuxR || Kelly Davis (DC) will this send to part BBa_I13263 || 1015 8F pSB3K3 || kan || growth MW || no
+
|[http://partsregistry.org/Part:BBa_C0072 BBa_C0072]||. mnt w/ LVA ||Plt.2-5M; pSB2K3 ||kan || ||  
 
|-
 
|-
−
|BBa_I13263 || Lux Receiver (HSL & R0063 driven)produces YFP || Pallavi Penumetcha (DC)|| 1002 8E pSB1A2 || amp || growth MW & DC || yes 210ng/ml
+
|[http://partsregistry.org/Part:BBa_C0040 BBa_C0040]||. tetR w/ LVA  ||Plt.1-5C; pSB1A2 ||amp || ||  
 
|-
 
|-
−
|BBa_f2622 || 3OC6HSL receiver that outputs PoPS || James Barron (DC) || 1001 12E pSB1A2 || amp || no growth || no
+
|[http://partsregistry.org/Part:BBa_C0062 BBa_C0062]||. LuxR no LVA  ||Plt.1-7A; pSB1A2 ||amp || ||  
 
|-
 
|-
−
|BBa_F1610 || Device that receives PoPS and outputs LuxI || Malcolm Campbell (DC) || 1016 10D pSB1AK3 || amp & kan || growth MW || no
+
|[http://partsregistry.org/Part:BBa_C0061 BBa_C0061]||. LuxI w/ LVA ||Plt.1-5O; pSB1A2 ||amp || ||  
 
|-
 
|-
−
|BBa_I0426 || Las-reciver(EYFP) || Kristi Muscalino (DC) || 1008 5B pSB1A2 || amp || growth MW & DC || yes 210ng/ml
+
|[http://partsregistry.org/Part:pSB4A3 BBa_pSB4A3]|| low copy amp plasmid || || || ||  
 
|-
 
|-
−
|BBa_I0407 || LasI test || Erin Feeney (DC) || 1008 4F pSB1A2 || amp || growth MW & DC || yes 76.37ng/ml
+
|BBa_|| || || || ||
 
|-
 
|-
−
|BBa_I0466|| RhlR Protein Generator without LVA || Laurie Heyer (DC) || 1001 5C pSB1A2 || amp || growth MW || yes 129.7ng/ml
+
|BBa_|| || || || ||  
 
|-
 
|-
−
|BBa_F2620|| Device receives 3OC6HSL outputs PoPS || Robert Cool (MW) || 1001 12D pSB1A2 || amp || growth MW || yes 83ng/ml
+
|BBa_|| || || || ||
 
|-
 
|-
 
|}
 
|}
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{| border="1" cellpadding="2"
 
{| border="1" cellpadding="2"
−
!width="50"|Part
+
!width="60"|Part
 
!width="225"|Description
 
!width="225"|Description
−
!width="225"|Student Responsible (DC or MW)
+
!width="50"|Location(07 registry) and Vector
−
!width="30"|Location and Vector
 
 
!width="30"|Resistance
 
!width="30"|Resistance
−
!width="30"|Results from 5/22/08
+
!width="30"|Results  
 +
!width="30"|miniprep
 +
|-
 +
|[http://partsregistry.org/Part:BBa_J06550 BBa_J06550]||. RBS+cI  ||Plt.2-9B; pSB1A2 ||amp ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_S0103 BBa_S0103]||. RBS+cI  ||Plt.1-15C; pSB1A2 || amp||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_I13025 BBa_I13025]||. RBS+mnt  ||Plt.1-23J; pSB1A2||amp ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_S0101 BBa_S0101]||. RBS+tetR  ||Plt.1-13O; pSB1A2||amp ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_J37033 BBa_J37033]||. RBS+LuxR(no LVA)  ||Plt.3-8F; pSB1A2  ||amp ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]||. RBS+LuxI  ||Plt.1-24A; pSB1A2 ||amp ||  ||
 +
|-
 +
|BBa_||  ||  ||  ||  ||
 +
|-
 +
|BBa_||  ||  ||  ||  || 
 +
|-
 +
|BBa_||  ||  ||  ||  ||
 +
|-
 +
|}
 +
 
 +
'''New Parts Constructed''':
 +
 
 +
{| border="1" cellpadding="2"
 +
!width="60"|Part
 +
!width="225"|Description
 +
!width="30"|Resistance
 +
!width="30"|Results
 
!width="30"|miniprep
 
!width="30"|miniprep
 +
!width="30"|glycerol stock
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091107 BBa_K091107]||. Lux-cI hybrid promoter  ||  || right size ||yes  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091100 BBa_K091100]||. pLac_lux hybrid promoter  ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091101 BBa_K091101]||. pTet_Lac hybrid promoter  ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091104 BBa_K091104]||. Mnt/LacI Hybrid Promoter  ||  ||  || ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091105 BBa_K091105]||. Mnt/TetR Promoter  ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091106 BBa_K091106]||. LsrA/cI hybrid promoter  ||  ||right size  ||yes  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091001 BBa_K091001]||. LsrR gene ||  ||  ||    ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091002 BBa_K091002]||. LsrK gene ||  ||  ||    ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091109 BBA_K091109]||. LuxS gene ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091110 BBA_K091110]||. LacI promoter ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091111 BBA_K091111]||. LacIQ promoter ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091112 BBA_K091112]||. LacIQ1 promoter ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091132 K091132] || pMnt/Tet+RBS+LuxI || amp || right size || yes ||yes
 
|-
 
|-
−
|BBa_F2621|| AHL receiver with lux pR and codes for LuxR || Karlesha Roland (DC) || 1001 12B pSB1A2 || amp || growth MW  ||no
+
|[http://partsregistry.org/Part:BBa_K091133 K091133] || pBAD+RBS+TetR ||amp || right size ||yes ||yes
 
|-
 
|-
−
|BBa_I15030 || Lux-sender(Autoinducing)Codes for LuxI and LuxR || Kelly Davis (DC)  will this send to part BBa_I13263 || 1015 8F pSB3K3 || kan || growth MW || no
+
|[http://partsregistry.org/Part:BBa_K091137 K091137] || RBS+Mnt+TT || amp || right size || yes ||yes
 
|-
 
|-
−
|BBa_I13263 || Lux Receiver (HSL & R0063 driven)produces YFP || Pallavi Penumetcha (DC)|| 1002 8E pSB1A2 || amp || growth MW & DC || yes 210ng/ml
+
|[http://partsregistry.org/Part:BBa_K091144 K091144] || pMnt/Tet+RBS+LuxI+RBS+cI+TT || amp || right size || yes ||yes
 
|-
 
|-
−
|BBa_f2622 || 3OC6HSL receiver that outputs PoPS || James Barron (DC) || 1001 12E pSB1A2 || amp || no growth || no
+
|[http://partsregistry.org/Part:BBa_K091145 K091145] || pBad+RBS+TetR+TT || amp || right size || yes || yes
 
|-
 
|-
−
|BBa_F1610 || Device that receives PoPS and outputs LuxI || Malcolm Campbell (DC) || 1016 10D pSB1AK3 || amp & kan || growth MW || no
+
|[http://partsregistry.org/Part:BBa_K091147 K091147] || pBAD+RBS+Mnt+TT || amp || right size || yes ||yes
 
|-
 
|-
−
|BBa_I0426 || Las-reciver(EYFP) || Kristi Muscalino (DC) || 1008 5B pSB1A2 || amp || growth MW & DC || yes 210ng/ml
+
|[http://partsregistry.org/Part:BBa_K091160 K091160] || pMnt/Tet+RBS+GFP+TT || amp || right size || yes || yes
 
|-
 
|-
−
|BBa_I0407 || LasI test || Erin Feeney (DC) || 1008 4F pSB1A2 || amp || growth MW & DC || yes 76.37ng/ml
+
| [http://partsregistry.org/Part:BBa_K091175 K091175] || K091145+K091160 || amp || right size || yes || yes
 
|-
 
|-
−
|BBa_I0466|| RhlR Protein Generator without LVA || Laurie Heyer (DC) || 1001 5C pSB1A2 || amp || growth MW || yes 129.7ng/ml
+
| [http://partsregistry.org/Part:BBa_K091176 K091176] || K091147+K091160 || amp || right size || yes || yes
 
|-
 
|-
−
|BBa_F2620|| Device receives 3OC6HSL outputs PoPS || Robert Cool (MW) || 1001 12D pSB1A2 || amp || growth MW || yes 83ng/ml
+
| [http://partsregistry.org/Part:BBa_K091177 K091177] || K091160+K091145 || amp || right size || yes || yes
 +
|-
 +
| [http://partsregistry.org/Part:BBa_K091178 K091178] || K091160+K091147 || amp || right size || yes ||yes
 
|-
 
|-
 
|}
 
|}

Latest revision as of 17:56, 6 August 2008

Basic Parts:

Part Description Location(07 registry) and Vector Resistance Results miniprep
BBa_C0051 . cI w/ LVA Plt.1-5G; pSB1A2 amp
BBa_C0072 . mnt w/ LVA Plt.2-5M; pSB2K3 kan
BBa_C0040 . tetR w/ LVA Plt.1-5C; pSB1A2 amp
BBa_C0062 . LuxR no LVA Plt.1-7A; pSB1A2 amp
BBa_C0061 . LuxI w/ LVA Plt.1-5O; pSB1A2 amp
BBa_pSB4A3 low copy amp plasmid
BBa_
BBa_
BBa_

Composite Parts:

Part Description Location(07 registry) and Vector Resistance Results miniprep
BBa_J06550 . RBS+cI Plt.2-9B; pSB1A2 amp
BBa_S0103 . RBS+cI Plt.1-15C; pSB1A2 amp
BBa_I13025 . RBS+mnt Plt.1-23J; pSB1A2 amp
BBa_S0101 . RBS+tetR Plt.1-13O; pSB1A2 amp
BBa_J37033 . RBS+LuxR(no LVA) Plt.3-8F; pSB1A2 amp
BBa_C0261 . RBS+LuxI Plt.1-24A; pSB1A2 amp
BBa_
BBa_
BBa_

New Parts Constructed:

Part Description Resistance Results miniprep glycerol stock
BBa_K091107 . Lux-cI hybrid promoter right size yes
BBa_K091100 . pLac_lux hybrid promoter
BBa_K091101 . pTet_Lac hybrid promoter
BBa_K091104 . Mnt/LacI Hybrid Promoter
BBa_K091105 . Mnt/TetR Promoter
BBa_K091106 . LsrA/cI hybrid promoter right size yes
BBa_K091001 . LsrR gene
BBa_K091002 . LsrK gene
BBA_K091109 . LuxS gene
BBA_K091110 . LacI promoter
BBA_K091111 . LacIQ promoter
BBA_K091112 . LacIQ1 promoter
K091132 pMnt/Tet+RBS+LuxI amp right size yes yes
K091133 pBAD+RBS+TetR amp right size yes yes
K091137 RBS+Mnt+TT amp right size yes yes
K091144 pMnt/Tet+RBS+LuxI+RBS+cI+TT amp right size yes yes
K091145 pBad+RBS+TetR+TT amp right size yes yes
K091147 pBAD+RBS+Mnt+TT amp right size yes yes
K091160 pMnt/Tet+RBS+GFP+TT amp right size yes yes
K091175 K091145+K091160 amp right size yes yes
K091176 K091147+K091160 amp right size yes yes
K091177 K091160+K091145 amp right size yes yes
K091178 K091160+K091147 amp right size yes yes
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