Difference between revisions of "RRNA operon"
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The 3' end of the 16S RNA, or small subunit, binds to the 5' end of the messenger RNA during transcription, specifically to the [http://en.wikipedia.org/wiki/Shine-Dalgarno_sequence Shine-Dalgarno sequence]. This aligns the mRNA's start codon for transcription. The 16S rRNA itself contains an anti-Shine-Dalgarno sequence on its 3' end. | The 3' end of the 16S RNA, or small subunit, binds to the 5' end of the messenger RNA during transcription, specifically to the [http://en.wikipedia.org/wiki/Shine-Dalgarno_sequence Shine-Dalgarno sequence]. This aligns the mRNA's start codon for transcription. The 16S rRNA itself contains an anti-Shine-Dalgarno sequence on its 3' end. | ||
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| + | Along with a promoter, a termination sequence, and an operator, the three seperate segments coding for the different subunits of the ribosome all form a single operon. The specific promoter and termination sequence is not known, but a [http://imgweb.jgi-psf.org/cgi-bin/img_edu_v260/main.cgi?section=ScaffoldGraph&page=scaffoldDna&scaffold_oid=2500575882&start_coord=2396557&end_coord=2397346 790-base-pair intergenetic region] exists upstream of the 16S coding region; the promoter sequence most likely resides there. | ||
Revision as of 05:27, 2 September 2008
The rRNA operon encodes for the ribosomal RNA, which comprises the ribosomes. Three distinct rRNA sequences exist in the operon, encoding for the two subunits of the ribosome; the 5S and 23S sequences code for the large subunit, while the 16S sequence encodes for the small subunit.
The three sequences are coded in the following order, from 3' to 5': 16S, 23S, 5S.
The 3' end of the 16S RNA, or small subunit, binds to the 5' end of the messenger RNA during transcription, specifically to the Shine-Dalgarno sequence. This aligns the mRNA's start codon for transcription. The 16S rRNA itself contains an anti-Shine-Dalgarno sequence on its 3' end.
Along with a promoter, a termination sequence, and an operator, the three seperate segments coding for the different subunits of the ribosome all form a single operon. The specific promoter and termination sequence is not known, but a 790-base-pair intergenetic region exists upstream of the 16S coding region; the promoter sequence most likely resides there.
| Gene Name | Gene Coordinates | Number of Base Pairs (Length) | Closest Match on BLAST (Homolog) | Score of Nearest Match | E-value of Nearest Match | Percent Identity to Nearest Match |
|---|---|---|---|---|---|---|
| 16S rRNA | 2397347..2398825 | 1479 bp | Halobacterium sp. NRC - 1 | 1966 | 0.0e+00 | 92.12% |
| Gap Bases | 2398825..2399190 | 365 | N/A | N/A | N/A | N/A |
| 23S rRNA | 2399190..2402100 | 2911 bp | Natronomonas pharaonis DSM 2160 | 1899 | 0.0e+00 | 90.06% |
| Gap Bases | 2402100..2402216 | 116 | N/A | N/A | N/A | N/A |
| 5S rRNA | 2402216..2402338 | 123bp | Halobacterium sp. NRC - 1 | 143 | 3.0e-36 | 90.00% |
