Difference between revisions of "Cellular Signaling Systems"
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Latest revision as of 14:42, 28 October 2008
Las/Rhl cell signaling system
Responsible: Robert Cool, Alicia Allen, and Erin Feeney
Las System
Signal Molecule: An AHL called PAI-1 (N-3-oxododecanoyl-l-hsl)(3-oxo-C12-hsl)
Bacterial species: Pseudomonas aeruginosa gram(-) possibly E.coli (see article 3)
Receiver protein: LasR
Effect of binding: TXN activation of virulence genes, lasA, lasB, apr, toxR
Synthase: LasI enzyme
Target Genes: lasI, lasA, lasB, apr, toxR
Regulation: unknown
References
"Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes"
JP Pearson, EC Pesci and BH Iglewski [1]
"Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa"
Dana S. Wade, M. Worth Calfee, Edson R. Rocha, Elizabeth A. Ling, Elana Engstrom, James P. Coleman, and Everett C. Pesci 2
Posttranscriptional Control of Quorum-Sensing-Dependent Virulence Genes by DksA in Pseudomonas aeruginosa
Florence Jude,Thilo Köhler,Pavel Branny,Karl Perron,Matthias P. Mayer,Rachel Comte, and Christian van Delden 3
Pending: [2]
Rhl System
Signal Molecule: An AHL called PAI-2, Plasminogen activator inhibitor-2, N-butanoyl-homoserine lactone (C4HSL)
Bacterial species: Pseudomonas aeruginosa, gram(-)
Receiver Protein: Rhl R
Effect of Binding: activation of Rhamnosyl Transferase, then making RL (rhamnolipid)
Synthase: RhlA and RhlB
Target Genes: pqsABCDE and phnAB
Regulation: unknown
References
background information on Las and Rhl
Parts Needed:
LasR + pro/term
RhlR + pro/term
RhlI + pro/term
pqsABCDE + pro/term
pqsR
pqsH + pro/term
phnAB
LasI
RBS
Lux cell signaling system
Responsible: Andrew Gordon and Pallavi Penumetcha
Signal molecule: N-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules
Bacterial species: discovered in Vibrio fischeri known to work in E. coli
Receiver protein: LuxR protein receives signal from AHL; also has some control over transciption of luciferase
Signal molecule synthase: LuxI; also has some control over transciption of luciferase
Additional Information: "Quorum Quenching" aiiA (intracellular) lactonase reduces AHL concentration
Resources
AHL signaling molecules by species; some are specific to gram pos but may affect gram negs
Reducing Crosstalk in Lux System
References
Quorum Quenching to control Lux Pathway
[3] A Synthetic multicellular system for programmed pattern formation
Parts Needed:
LuxR + pro/term
RBS
LuxI + pro/term
LuxI sender
