Difference between revisions of "WillSequencing"

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{| border="1" cellpadding="5" cellspacing="0" align="center" width="100%"
 
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|wcd001|| 6/24/2008||Bca1109AC|| 1 || ca998|| bad read || [[AndersonLab_wcdseq-wcd001| wcd001]]
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|'''Reaction Name'''||'''Date'''||'''Part Name'''||'''Primer'''||'''Result'''||'''Sequence File'''
 
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|wcd002|| 6/24/2008||Bca1265AC|| 1 || ca998|| bad read || [[AndersonLab_wcdseq-wcd002| wcd002]]
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|pLuxLas1|| 10/06/08||BBa_K091157|| G00100 (VF2)|| 2 mutations [C->T, del T]|| [[wcdseq_pLuxLas1| pLuxLas1]]
 
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|wcd003|| 6/27/08||Bca1265AC|| 1 || ca998|| perfect (miscall - C to G) || [[AndersonLab_wcdseq-wcd003| wcd003]]
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|pLuxLas2|| 10/06/08||BBa_K091157|| G00100 (VF2)|| 3 mutations [del C, del T, del A] || [[wcdseq_pLuxLas2| pLuxLas2]]
 
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|wcd004|| 6/27/08||Bca1265KA|| 1 || ca998|| perfect || [[AndersonLab_wcdseq-wcd004| wcd004]]
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|pLuxLas3|| 10/06/08||BBa_K091157|| G00100 (VF2)|| 2 mutations [T -> A, del A] || [[wcdseq_pLuxLas3| pLuxLas3]]
 
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|wcd005|| 6/27/08||Bca1092KC|| 1 || ca998|| perfect || [[AndersonLab_wcdseq-wcd005| wcd005]]
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|B0015_VF2|| 10/06/08||BBa_B0015|| G00100 (VF2)|| perfect || [[wcdseq_B0015_VF2| B0015_VF2]]
 
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|wcd006|| 6/27/08||Bca1266AC|| 1 || ca998|| perfect || [[AndersonLab_wcdseq-wcd006| wcd006]]
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|J23100_VF2|| 10/06/08||BBa_J23100|| G00100 (VF2)|| perfect || [[wcdseq_J23100_VF2| J23100_VF2]]
 
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|wcd007|| 6/27/08||Bca1266KA|| 1 || ca998|| No Match (mRFP still there) || [[AndersonLab_wcdseq-wcd007| wcd007]]
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|S03954_VR|| 10/06/08||BBa_S03954|| G00101 (VR)|| perfect || [[wcdseq_S03954_VR| S03954_VR]]
 
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|wcd008|| 6/27/08||Bca1229AK|| 1 || ca998|| Nonsense Mutation (A to G  at ~405) || [[AndersonLab_wcdseq-wcd008| wcd008]]
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|S03983_VF2|| 10/06/08||BBa_S03983|| G00100 (VF2)|| wrong part (appears to be S03982 with a nonsense mutation in LasR) || [[wcdseq_S03983_VF2| S03983_VF2]]
 
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|wcd009|| 6/27/08||Bca1229KA|| 1 || ca998|| Nonsense Mutation (A to G at ~405) || [[AndersonLab_wcdseq-wcd009| wcd009]]
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|S03983_VR|| 10/06/08||BBa_S03983|| G00101 (VR)|| wrong part (appears to be S03982 with a nonsense mutation in LasR) || [[wcdseq_S03983_VR| S03983_VR]]
 
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|wcd010|| 6/27/08||Bca1270CK|| 1 || ca998|| perfect || [[AndersonLab_wcdseq-wcd010| wcd010]]
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|pLuxprime-VR|| 03/09/09||pSB1A2-pLux'|| G00101 (VR)|| wrong part (unclear what this is, but it was too long and contained no XbaI/SpeI)|| [[wcdseq_pLuxprime_VR| pLuxprime-VR]]
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|pLuxLas-VR|| 03/09/09||pSB1A2-pLuxLas|| G00101 (VR)|| wrong part (1 bp too long. [http://gcat.davidson.edu/GcatWiki/index.php/Image:PLuxLasBlast.png 100% blast hit with genomic DNA], including ExoRI/PstI site) || [[wcdseq_pLuxLas_VR| pLuxLas-VR]]
 
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Latest revision as of 22:56, 14 March 2009

Reaction Name Date Part Name Primer Result Sequence File
pLuxLas1 10/06/08 BBa_K091157 G00100 (VF2) 2 mutations [C->T, del T] pLuxLas1
pLuxLas2 10/06/08 BBa_K091157 G00100 (VF2) 3 mutations [del C, del T, del A] pLuxLas2
pLuxLas3 10/06/08 BBa_K091157 G00100 (VF2) 2 mutations [T -> A, del A] pLuxLas3
B0015_VF2 10/06/08 BBa_B0015 G00100 (VF2) perfect B0015_VF2
J23100_VF2 10/06/08 BBa_J23100 G00100 (VF2) perfect J23100_VF2
S03954_VR 10/06/08 BBa_S03954 G00101 (VR) perfect S03954_VR
S03983_VF2 10/06/08 BBa_S03983 G00100 (VF2) wrong part (appears to be S03982 with a nonsense mutation in LasR) S03983_VF2
S03983_VR 10/06/08 BBa_S03983 G00101 (VR) wrong part (appears to be S03982 with a nonsense mutation in LasR) S03983_VR
pLuxprime-VR 03/09/09 pSB1A2-pLux' G00101 (VR) wrong part (unclear what this is, but it was too long and contained no XbaI/SpeI) pLuxprime-VR
pLuxLas-VR 03/09/09 pSB1A2-pLuxLas G00101 (VR) wrong part (1 bp too long. 100% blast hit with genomic DNA, including ExoRI/PstI site) pLuxLas-VR
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