Difference between revisions of "WillSequencing"
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| − | {| border="1" cellpadding="5" cellspacing="0" align="center" width=" | + | {| border="1" cellpadding="5" cellspacing="0" align="center" width="100%" |
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|'''Reaction Name'''||'''Date'''||'''Part Name'''||'''Primer'''||'''Result'''||'''Sequence File''' | |'''Reaction Name'''||'''Date'''||'''Part Name'''||'''Primer'''||'''Result'''||'''Sequence File''' | ||
|- | |- | ||
| − | |pLuxLas1|| 10/06/08||BBa_K091157|| G00100 (VF2)|| 2 mutations | + | |pLuxLas1|| 10/06/08||BBa_K091157|| G00100 (VF2)|| 2 mutations [C->T, del T]|| [[wcdseq_pLuxLas1| pLuxLas1]] |
|- | |- | ||
| − | |pLuxLas2|| 10/06/08||BBa_K091157|| G00100 (VF2)|| 3 mutations | + | |pLuxLas2|| 10/06/08||BBa_K091157|| G00100 (VF2)|| 3 mutations [del C, del T, del A] || [[wcdseq_pLuxLas2| pLuxLas2]] |
|- | |- | ||
| − | |pLuxLas3|| 10/06/08||BBa_K091157|| G00100 (VF2)|| 2 mutations | + | |pLuxLas3|| 10/06/08||BBa_K091157|| G00100 (VF2)|| 2 mutations [T -> A, del A] || [[wcdseq_pLuxLas3| pLuxLas3]] |
|- | |- | ||
|B0015_VF2|| 10/06/08||BBa_B0015|| G00100 (VF2)|| perfect || [[wcdseq_B0015_VF2| B0015_VF2]] | |B0015_VF2|| 10/06/08||BBa_B0015|| G00100 (VF2)|| perfect || [[wcdseq_B0015_VF2| B0015_VF2]] | ||
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|S03954_VR|| 10/06/08||BBa_S03954|| G00101 (VR)|| perfect || [[wcdseq_S03954_VR| S03954_VR]] | |S03954_VR|| 10/06/08||BBa_S03954|| G00101 (VR)|| perfect || [[wcdseq_S03954_VR| S03954_VR]] | ||
|- | |- | ||
| − | |S03983_VF2|| 10/06/08||BBa_S03983|| G00100 (VF2)|| wrong part || [[wcdseq_S03983_VF2| S03983_VF2]] | + | |S03983_VF2|| 10/06/08||BBa_S03983|| G00100 (VF2)|| wrong part (appears to be S03982 with a nonsense mutation in LasR) || [[wcdseq_S03983_VF2| S03983_VF2]] |
|- | |- | ||
| − | |S03983_VR|| 10/06/08||BBa_S03983|| G00101 (VR)|| wrong part || [[wcdseq_S03983_VR| S03983_VR]] | + | |S03983_VR|| 10/06/08||BBa_S03983|| G00101 (VR)|| wrong part (appears to be S03982 with a nonsense mutation in LasR) || [[wcdseq_S03983_VR| S03983_VR]] |
| + | |- | ||
| + | |pLuxprime-VR|| 03/09/09||pSB1A2-pLux'|| G00101 (VR)|| wrong part (unclear what this is, but it was too long and contained no XbaI/SpeI)|| [[wcdseq_pLuxprime_VR| pLuxprime-VR]] | ||
| + | |- | ||
| + | |pLuxLas-VR|| 03/09/09||pSB1A2-pLuxLas|| G00101 (VR)|| wrong part (1 bp too long. [http://gcat.davidson.edu/GcatWiki/index.php/Image:PLuxLasBlast.png 100% blast hit with genomic DNA], including ExoRI/PstI site) || [[wcdseq_pLuxLas_VR| pLuxLas-VR]] | ||
|} | |} | ||
Latest revision as of 22:56, 14 March 2009
| Reaction Name | Date | Part Name | Primer | Result | Sequence File |
| pLuxLas1 | 10/06/08 | BBa_K091157 | G00100 (VF2) | 2 mutations [C->T, del T] | pLuxLas1 |
| pLuxLas2 | 10/06/08 | BBa_K091157 | G00100 (VF2) | 3 mutations [del C, del T, del A] | pLuxLas2 |
| pLuxLas3 | 10/06/08 | BBa_K091157 | G00100 (VF2) | 2 mutations [T -> A, del A] | pLuxLas3 |
| B0015_VF2 | 10/06/08 | BBa_B0015 | G00100 (VF2) | perfect | B0015_VF2 |
| J23100_VF2 | 10/06/08 | BBa_J23100 | G00100 (VF2) | perfect | J23100_VF2 |
| S03954_VR | 10/06/08 | BBa_S03954 | G00101 (VR) | perfect | S03954_VR |
| S03983_VF2 | 10/06/08 | BBa_S03983 | G00100 (VF2) | wrong part (appears to be S03982 with a nonsense mutation in LasR) | S03983_VF2 |
| S03983_VR | 10/06/08 | BBa_S03983 | G00101 (VR) | wrong part (appears to be S03982 with a nonsense mutation in LasR) | S03983_VR |
| pLuxprime-VR | 03/09/09 | pSB1A2-pLux' | G00101 (VR) | wrong part (unclear what this is, but it was too long and contained no XbaI/SpeI) | pLuxprime-VR |
| pLuxLas-VR | 03/09/09 | pSB1A2-pLuxLas | G00101 (VR) | wrong part (1 bp too long. 100% blast hit with genomic DNA, including ExoRI/PstI site) | pLuxLas-VR |
