Difference between revisions of "WillSequencing"

Latest revision as of 22:56, 14 March 2009

Reaction Name	Date	Part Name	Primer	Result	Sequence File
pLuxLas1	10/06/08	BBa_K091157	G00100 (VF2)	2 mutations [C->T, del T]	pLuxLas1
pLuxLas2	10/06/08	BBa_K091157	G00100 (VF2)	3 mutations [del C, del T, del A]	pLuxLas2
pLuxLas3	10/06/08	BBa_K091157	G00100 (VF2)	2 mutations [T -> A, del A]	pLuxLas3
B0015_VF2	10/06/08	BBa_B0015	G00100 (VF2)	perfect	B0015_VF2
J23100_VF2	10/06/08	BBa_J23100	G00100 (VF2)	perfect	J23100_VF2
S03954_VR	10/06/08	BBa_S03954	G00101 (VR)	perfect	S03954_VR
S03983_VF2	10/06/08	BBa_S03983	G00100 (VF2)	wrong part (appears to be S03982 with a nonsense mutation in LasR)	S03983_VF2
S03983_VR	10/06/08	BBa_S03983	G00101 (VR)	wrong part (appears to be S03982 with a nonsense mutation in LasR)	S03983_VR
pLuxprime-VR	03/09/09	pSB1A2-pLux'	G00101 (VR)	wrong part (unclear what this is, but it was too long and contained no XbaI/SpeI)	pLuxprime-VR
pLuxLas-VR	03/09/09	pSB1A2-pLuxLas	G00101 (VR)	wrong part (1 bp too long. 100% blast hit with genomic DNA, including ExoRI/PstI site)	pLuxLas-VR

@@ Line 1: / Line 1: @@
-{| border="1" cellpadding="5" cellspacing="0" align="center" width="80%"
+{| border="1" cellpadding="5" cellspacing="0" align="center" width="100%"
 |-
 |'''Reaction Name'''||'''Date'''||'''Part Name'''||'''Primer'''||'''Result'''||'''Sequence File'''
@@ Line 15: / Line 15: @@
 |S03954_VR|| 10/06/08||BBa_S03954|| G00101 (VR)|| perfect || [[wcdseq_S03954_VR| S03954_VR]]
 |-
-|S03983_VF2|| 10/06/08||BBa_S03983|| G00100 (VF2)|| wrong part || [[wcdseq_S03983_VF2| S03983_VF2]]
+|S03983_VF2|| 10/06/08||BBa_S03983|| G00100 (VF2)|| wrong part (appears to be S03982 with a nonsense mutation in LasR) || [[wcdseq_S03983_VF2| S03983_VF2]]
 |-
-|S03983_VR|| 10/06/08||BBa_S03983|| G00101 (VR)|| wrong part || [[wcdseq_S03983_VR| S03983_VR]]
+|S03983_VR|| 10/06/08||BBa_S03983|| G00101 (VR)|| wrong part (appears to be S03982 with a nonsense mutation in LasR) || [[wcdseq_S03983_VR| S03983_VR]]
+|-
+|pLuxprime-VR|| 03/09/09||pSB1A2-pLux'|| G00101 (VR)|| wrong part (unclear what this is, but it was too long and contained no XbaI/SpeI)|| [[wcdseq_pLuxprime_VR| pLuxprime-VR]]
+|-
+|pLuxLas-VR|| 03/09/09||pSB1A2-pLuxLas|| G00101 (VR)|| wrong part (1 bp too long. [http://gcat.davidson.edu/GcatWiki/index.php/Image:PLuxLasBlast.png 100% blast hit with genomic DNA], including ExoRI/PstI site) || [[wcdseq_pLuxLas_VR| pLuxLas-VR]]
 |}