Difference between revisions of "Hypothetical Protein 644029933 (Olivia Ho-Shing)"
From GcatWiki
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The nucleotide sequence for this gene does have a start codon (ATG) and stop codon (TGA) defined. The BLASTn hits for the nucleotide sequence, other than a perfect alignment with itself (Halomicrobium mukohataei complete genome), returned hits with poor Query coverage (<10%) and unreliable E-values (>0.05). <br> | The nucleotide sequence for this gene does have a start codon (ATG) and stop codon (TGA) defined. The BLASTn hits for the nucleotide sequence, other than a perfect alignment with itself (Halomicrobium mukohataei complete genome), returned hits with poor Query coverage (<10%) and unreliable E-values (>0.05). <br> | ||
[[Image:hyp-blastn|500px|center]]<br> | [[Image:hyp-blastn|500px|center]]<br> | ||
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| + | For the BLASTp hits, there were several hits for a monooxygenase protein, but the E-values were very unreliable. | ||
| + | <br>[[Image:hyp-blastp|500px|center]]<br> | ||
| + | [[Image:hyp-blastp-align|thumb|500px|center]]<br> | ||
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| + | == Kyte-Doolittle Plot == | ||
| + | Kyte Doolittle plots | ||
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| + | == PREDATOR == | ||
| + | a [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=protein&dopt=GenPept&RID=AFEVZ8ST012&log%24=prottop&blast_rank=6&list_uids=167567057 monooxygenase] from the BLASTp hits. | ||
Revision as of 14:35, 9 September 2009
I randomly chose a protein with no predicted function: 644029933 hypothetical protein
In order to search for some possible function I tried using:
- BLASTn
- BLASTp
- Kyte-Doolittle Plot
- PREDATOR
BLASTn and BLASTp
The nucleotide sequence for this gene does have a start codon (ATG) and stop codon (TGA) defined. The BLASTn hits for the nucleotide sequence, other than a perfect alignment with itself (Halomicrobium mukohataei complete genome), returned hits with poor Query coverage (<10%) and unreliable E-values (>0.05).
For the BLASTp hits, there were several hits for a monooxygenase protein, but the E-values were very unreliable.
File:Hyp-blastp-align
500px
Kyte-Doolittle Plot
Kyte Doolittle plots
PREDATOR
a monooxygenase from the BLASTp hits.
