Difference between revisions of "Pouring an Agarose Gel"
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| − | + | '''Pouring an Agarose Gel''' | |
| − | + | ||
| − | + | # Add 35 mL of 1X TAE buffer to a 125 ml flask | |
| − | + | # Add agarose (0.35 grams agarose for 1% gel). | |
| − | + | # Microwave for 50 seconds | |
| − | + | # Swirl and microwave for 5 seconds - repeat 3 times | |
| + | # Place flask in 55 C water bath to cool | ||
| + | # Add 5 µL of 10 mg/ml Ethidium Bromide (CAUTION: WEAR GLOVES) | ||
| + | # Swirl to mix and pour into gel tray with comb | ||
Latest revision as of 19:03, 15 February 2017
Pouring an Agarose Gel
- Add 35 mL of 1X TAE buffer to a 125 ml flask
- Add agarose (0.35 grams agarose for 1% gel).
- Microwave for 50 seconds
- Swirl and microwave for 5 seconds - repeat 3 times
- Place flask in 55 C water bath to cool
- Add 5 µL of 10 mg/ml Ethidium Bromide (CAUTION: WEAR GLOVES)
- Swirl to mix and pour into gel tray with comb
