Difference between revisions of "Malcolm C"

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I will also be working on the 13 genes Allan gave us.  
 
I will also be working on the 13 genes Allan gave us.  
  
 +
 +
== SSR Primer Hunt ==
 
The class has already completed 4 SSR primer designs (LDOX, CHS, StSy, and F3H).
 
The class has already completed 4 SSR primer designs (LDOX, CHS, StSy, and F3H).
  
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# Forward Primer GAGCTCTTGGATGCTCCATACT  & Rev Primer CCTCATAATTTTTGGTCTTCGC (taa) x6 PCR product = 137 bp
 
# Forward Primer GAGCTCTTGGATGCTCCATACT  & Rev Primer CCTCATAATTTTTGGTCTTCGC (taa) x6 PCR product = 137 bp
 
# Forward Primer TATCCCATGTTTTGGCTAATCC  & Rev Primer TTTGCTTTTCAATCGGGAAC (tc) x9 PCR product = 261 bp
 
# Forward Primer TATCCCATGTTTTGGCTAATCC  & Rev Primer TTTGCTTTTCAATCGGGAAC (tc) x9 PCR product = 261 bp
 +
  
 
'''AOMT scaffold 01781'''
 
'''AOMT scaffold 01781'''
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−
'''CHI scaffold03186'''
+
'''CHI scaffold 03186'''
 
# Forward Primer TACATCTTGAGGGGCAGTTTTT  & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
 
# Forward Primer TACATCTTGAGGGGCAGTTTTT  & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
 
# Forward Primer ACGTGGTCCAATCTAACCTCAC  & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp
 
# Forward Primer ACGTGGTCCAATCTAACCTCAC  & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp
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# Forward Primer TACATCTTGAGGGGCAGTTTTT  & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
 
# Forward Primer TACATCTTGAGGGGCAGTTTTT  & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
 
# Forward Primer ACGTGGTCCAATCTAACCTCAC  & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp
 
# Forward Primer ACGTGGTCCAATCTAACCTCAC  & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp
 +
  
 
'''F3'H scaffold 01438'''
 
'''F3'H scaffold 01438'''
 
# Forward Primer CAGTGGTACAGTGGTGGATGAC  & Rev Primer ATTCCCTTTCTCTTCCCATTTC (ag) x7 PCR product = 152 bp
 
# Forward Primer CAGTGGTACAGTGGTGGATGAC  & Rev Primer ATTCCCTTTCTCTTCCCATTTC (ag) x7 PCR product = 152 bp
 
# Forward Primer ATACCAATCCATAGTCTCCCGA  & Rev Primer TCAGTTGTGTTTTTCAGTTGGG (ta) x7 PCR product = 279 bp
 
# Forward Primer ATACCAATCCATAGTCTCCCGA  & Rev Primer TCAGTTGTGTTTTTCAGTTGGG (ta) x7 PCR product = 279 bp
 +
  
 
'''FLS scaffold 00142'''
 
'''FLS scaffold 00142'''
 +
# Forward Primer CAATTATACCCTTCCTGTCCCA  & Rev Primer GAATGGATGAAAGGAACCCATA (ag) x8 PCR product = 214 bp
 +
# Forward Primer CTAGCTCTCCCTCTCTCCCTCT  & Rev Primer GTGGTACTCCATATCCATGCCT (ct) x8 PCR product = 295 bp
 +
  
 
'''FLS scaffold 00374'''
 
'''FLS scaffold 00374'''
 +
# Forward Primer TGAGCATCATTGCCAGAGTAGT  & Rev Primer GGATTTCATTCCAAGAGATTGC (ct) x6 PCR product = 295 bp
 +
# Forward Primer TAGATTATCCGGGGAATCATTG  & Rev Primer GATTCTCATGACAAGCCAGTTG (tc) x6 PCR product = 275 bp
 +
  
 
'''MYBA1 scaffold 03500'''
 
'''MYBA1 scaffold 03500'''
 +
# Forward Primer GCGAGGATGGAAATATATACGG  & Rev Primer GCTTCATAAGCTGCTAGGCAAT (ag) x8 PCR product = 222 bp
 +
# Forward Primer GCGAGGATGGAAATATATACGG  & Rev Primer GCTTCATAAGCTGCTAGGCAAT (ttat) x5 PCR product = 222 bp
 +
  
 
'''MYCA1 scaffold 00278'''
 
'''MYCA1 scaffold 00278'''
 +
# Forward Primer CAGTATTTGAGCCGGAACCTAC  & Rev Primer CGTACAGAGTTTCGCACAAAAG (ct) x7 PCR product = 268 bp
 +
# Forward Primer TAGTGGGAACGAGTCGTCTTTT  & Rev Primer AGTTGGTGCCCTTTTATGAAGA (at) x6 PCR product = 195 bp
 +
  
 
'''MYCA1 scaffold 00554'''
 
'''MYCA1 scaffold 00554'''
 +
# Forward Primer GGCTAAAACCCTAGTTGCCTTT  & Rev Primer GGGTGTACTGAGAACCGAAGTC (tc) x6 PCR product = 157 bp
 +
# Forward Primer CCCAAAAGAAAATACCTGGACA  & Rev Primer GGAGCTTGGACCTAAAAGGATT (ga) x8 PCR product = 260 bp
 +
  
 
'''UFGT scaffold 00304'''
 
'''UFGT scaffold 00304'''
 +
# Forward Primer TGAACTACCAAAATGGGACAGA  & Rev Primer NGACGGAGCACATCCTACTACA (ag) x10 PCR product = 207 bp
 +
# Forward Primer CCCCAAATTCAGATCAAAGAAG  & Rev Primer CTTATCTCTGCCCACCTACACC (ag) x12 PCR product = 230 bp
 +
  
 
'''UFGT scaffold 01120'''
 
'''UFGT scaffold 01120'''
 +
# Forward Primer TGTGTGTGTGTAGTGTGGTCGT  & Rev Primer AAGAGAGAGGGGTCCTGAAAAC (ct) x11 PCR product = 132 bp
 +
# Forward Primer TAAGCTACAGTACCTCGCCACA  & Rev Primer AAGGATATGCCGACGAGTCTTA (tc) x6 PCR product = 196 bp
 +
 +
 +
 +
== EST Search Results ==
 +
 +
 +
For the second half of the semester, we focused on finding ESTs of our genes using the [http://dev.vaccinium.org/ Washington State University Vaccinium genome database] as well as the [http://bioinformatics.towson.edu/BBGD454/ Towson State University Blue Berry Genome database] using 454 sequencing.<br>
 +
 +
Download my PPT summary.[[Media:Allans_ESTs.pptx]]
 +
 +
 +
ANPER - anthocyanin permease
 +
 +
AOMT - anthocyanin O-methyltransferase
 +
 +
CHI - Chalcone isomerase
 +
 +
DFR - dihydroflavonol 4-reductase
 +
 +
F3’H flavonoid 3' hydroxylase
 +
 +
LDOX - leucoanthocyanidin dioxygenase
 +
 +
Myba1 - myb-related transcription factor
 +
 +
MycA1 - myc anthocyanin regulatory protein
 +
 +
UFGT - UDP-glucose:flavonoid 3-O-glucosyltransferase
 +
 +
 +
1) ANPER
 +
Submitted the 3’ most blueberry sequence from my scaffold00666<br>
 +
ggatggcaagctctggtagcttacatcaacttgggatcctattacat<br>
 +
Got this result from BLASTn search of Vaccinium database<br>
 +
>DR067294 <br>         
 +
Length = 260  Score = 60.0 bits (30), Expect = 9e-11  Identities = 39/42 (92%)  Strand = Plus / Plus                                                       
 +
[[File:DR067294.png]]
 +
 +
 +
2) AOMT 01781 search of Vaccinium database<br>
 +
Submitted carboxyl most amino acids that gave hits with my scaffold01781<br>
 +
Did tBLASTn<br>
 +
EEMVLEDLRPSRESTMKLNKMLAADPRVMISHASIGDGITLCMRV<br>
 +
No hits.<br>
 +
 +
I submitted <br>
 +
ATCGAGCTAATATTTTATGAGACcACTTAAAAAaTTTAAAAAaTTATTTtAATATTAATGAACGATTCGGCTCATTTGTACTCTACGGA<br>
 +
From the scaffold  <br>
 +
 +
And got <br>
 +
>DW043195          <br>
 +
Length = 523  Score = 48.1 bits (24), Expect = 7e-07  Identities = 33/36 (91%)  Strand = Plus / Minus                <br>                               
 +
[[File:DW043195.png]]
 +
 +
 +
3) AMOT 00796 search
 +
submitted
 +
ATCTAAAAATAAATAAATAATTTGAGTAATAATTATTAAAGACGAAAAAaTAAaGATGACGAAATAGTAAAAGCTTTGGAAGATAAATTTTCAAACCTGGAAACCCGCAAATTACTCCCACTTGCATATATAAGTACTCA<br>
 +
TATAATTTTCTATTATTTAATTTAATGGGTCCTAACATGATTGTAGTTATTATTTTTTTTtATTATTATTGTAGTTATTTATACTACGCAAGATGAAGGGTGCACAAGTCTTCCATGTTGTGGAATATCGCAGCTACAAGCA<br>
 +
TTTATTTAATGGGTACAAAATATTTTTGTAAGCGTCGtGCCCATTATAGGTGAGCATATAAGTCTATAAAGTATTAATTTAGAGAGGTCAATTAAAAAaGAaTCTCCCTGTGACACTACAATCGTGCTTGATGTCAACAAa<br>
 +
TATGaCAAAAAAaTATATaTAAAaTaTAAAacAAgTACTTtCAtgcATtATTtaTTtAAtATTTttCAcACAAATTTGAAATTGATGCCGAAAAATTAGAAATTTCACATAAAATATTTATTTAAaTTAAAAATAGCACGGTTTTTC<br>
 +
ATAAGGAACTAAAATTACTTGGCGGACGGAATACCAGAAAATCACGCAATTAATTCTTCAaGTTTTTCACTTTTCACGGAGGTGGGTTTATGATCtCGTAAATAAGTAGTTTCTTCTTTTTTtATTGTGGATATAATCTCCT<br>
 +
TCTATTTTCTCTAAATATTGAGGATCATGATAAtGCAAAGATGCAAGTGGTGGCTAGCTCATGCGGTAAGAGCTCTTGGATGCTCCATACTGCATCCCGAGATCGAGTCCCATCTCCCTTCCTAATGTAATAAATCAGGA<br>
 +
ATGATGAATGAAAAaTAATACGGAGTAATAATAATAATAATAATGCGAAGACCAAAAATTATGAGGATTTTTTtAGGAGGGTTtCGAATTACACCATCcTTTTTGGTAGGTGAATGGTTCAGTGCATGTGAGCATTTTAAT<br>
 +
AAAATCATTACATATTTTGGtATCAAATGGTATTAGTATATATAATTAATTAATTAAGTAATTATGACCCCTTGCATGTGAATGAAGGTGGTGGTGGGACCACCAACACCAATACCAACTCAACGGCATATACATATTGG<br>
 +
AATCGTCAATAGcGGGCAAGTGTGGTTGGTGGAaGgTTGGTGAAATGAAACTTTaGtCcACGTAACAaGAAAaGAGTTGGTGGCCATCCCcTTCAAGGGATATCATTTTCTTTTtAAAGGGCATaTAAAAAAAAAaTtAAAaT<br>
 +
cACTAACAAGAAATTTTAAGGAAAaTAAaCAaGCCCAACcaTAAAACGGTAgATACCAAaTCTATACCACCTTATAGTATATCTATTTTGgAATTtCACACTAgTGATCAAACGGaTGTGGGgTTgCTGCTATGCAGCCCTAG<br>
 +
CACAACAAGTGCTGTGTATTTGGCTTGTTCGTTACTGGGATGGACggCCcAGATTTTACCtCGGCACCTTTGCATCGAatCGTTTGTtGTCGTGCGTGCAAGTGAAATCTGGATCGTCCATCAGAGCAACGGATGACCCGGA<br>
 +
TGTGCATAGCACCTGCTGTGCACGGGGgTgCACAACAGCAACCCcAGTTCTGATCAAACcAATTCTATTATTTtAAaGATTATTTTATGGgTCTTtGAAaGAAAAAAAaaCTCATTCGGATGTCGTTAAGGGCTTAATAGGAC<br>
 +
GAAAATCGATCAAGCATTTAAtGATATtTGAATGAGTTGATTTTTTataGagACTCTtAAGAAtaTGTTTTAAATCAATTGAAtGACTCTgaTCATTTTTGTGGAATTCCAAAATAATTTTtACACGATAGGGTAAATATTTGGCa<br>
 +
TACACCACATGGTATAaGCAtAGTTTTTTTTTTTttGgtGCCTTTACTTGgTTCATTTCCTTTATTTTTGCGAGTCTAAGTCATTATCGTTTCTAGGGATGGCAACGGGTAAgaCATGGATCAGGGATCCTATTTTACCTTCGA<br>
 +
TCCTTAAATGGGAATTTCCTTTCCACCCCcACcTCCATATCTAAAAGGGaTAGTgTTttCCAcgATACCCGCCTCCCAATGGGGATCGgaTACACTtGGGTAAATtcATATCCAAACTAGTTGAAAAAaaGAACAaTAAGATCT<br>
 +
AAAtAAAAATAaTTtaCgTGTCC<br>
 +
 +
BLASTn<br>
 +
Got 3 EST hits<br>
 +
 +
[[File:AMOT_00796.png]]
 +
 +
 +
 +
 +
4) CHI
 +
 +
I did BLASTn with (from scaffold near SSR primer)
 +
TTTCTTGAGGTCTTCCAGGATGAGACATTCCCCCCTGGTGCTTCTATTCTTTTCACCCAGTCACCCCATGGGTTGCTAACGGTCAGTACATCCTTCCACAAGTGTTAGTTCTGCCTTTCTCTTCGCCTTTtGTTCTCTCTTC<br>
 +
CTTGTTGATTATAAAATAGGTAATGCAGTTCCTTGATTCAGGAACAATTTAAGCAGGTATCCAAATTTTCTTTtGAAaTTTGAGGgCAAGGAAATCACGATGATTTATGAGACTGATGTTGCATGTTAATTtCAGCTTGTAC<br>
 +
CCCAGATGCAGCATCGATCAGGACATTGTGAATTTCTTTtGTTTTtGGTCACCATTACTTGGTTCTCTACTAGTTgACGGTTCACTGTGCACTAaGCTCCATTTTGATGGACTTCATAAAACaCGATGCAGGACAagATGTA<br>
 +
AACCAGGCCCATGAAAATAGTTtCGTGTTTTAAATCaTTAAGCCTGTTCTTGACTTCGCCAAGAAGAtCAAACTCGTAGTATAAAGTATGTTGTTGAGAAGtGTTCTGAAAAGTAAGcAAATGGGTTTCTATTTtGGTTTTCA<br>
 +
TCTTTTTATAACACTCTTGGTTAATTTTtACCCTAAAAAaTACTCcATTAaTtAAGACCTAAAGACAAcTGGTTTGGGTtGATTATAGGTTTACTTTTATCTGTTtGGTGACATCACTAGATaGGCTGTGTTGCGACCTTGCCGA<br>
 +
TTGGTTTtCACAATCGGAGCACCTGAGTTATGAAGAGACATGCTTTTTtGAGGAACTTTAGGCCCGATATCGTCTTAACTCTTGAGAATCATTTTCTTCATTGATTTGGACATACACACACATGCATTCGAATCCGTGGTGC<br>
 +
AAATGATTCTGTAAGATACAGGTCATTTGAATCTTCATGCTCAAAACCCCCATTCAATTAGCGACTTACTTCTTACTTCCCAAGGAGCAGGAACTGCTCTTTTAACCCTTCTCCGAGGATTACATCCACAAACACAAAGTC<br>
 +
GATGGCAGACTGATATTACTCATCATCATTATCTATTGCATTTATTTTTCTTGTTGCTGATCATATGTATTTGACAAACTTTGGGATTACATGAGTATGTACATCCACAGATATGCATTGGTGTTGCGCGTGTATGTAGGAA<br>
 +
GTTGCAATGAAACAATAACGTTTTGATTTCCTATTGTTGATGCTTTATTTTCTGACTACAGATTGGCTTCTCGAAAGATGGTTTGTTACCTGAGACTGGCAAAGTGGTAATCGAGAACAAACAATTGTCCGAAGCAGTGTT<br>
 +
GGAATCAATCATCGGGAAGCATGGCGTTTCCCcTGCAGCAAAGCAGAGTTTGGCGGCAAGAGTgtCTGCATTGTTCAAGGAGGCATAAGAAAGAAGGTTTTGGAAAACGATAAGAAAGAAGTCCTGTAGCAGAAAagttAg<br>
 +
TAGtAGTTtATTGAgagaGGAAGATAAAATGGTAGAATCTCACTTCCCAGTTGATTCTGATGTTTCTCCATGAGTAAATGTATCTGTACGCTCTTTCATTTCCGTTTtGTCACTGTAATAAGAAGGCTTCAaCTTTTGCATTCT<br>
 +
AAACTCATCACATCTATCTACGACCTTTTCTTATTCTTCTTTgCAAATTTAAACCAAACcGagCCTTATGtCCCAATAaaTTGGGgTCTACTATTGGGgTCTACTATGtAAatCCcTTTTTttGtCcATTGGgATCTGtGCAAATTC<br>
 +
AAATGATATTGAAATTAAaTTCGGCATAaTGGCGGTAGTGGTTATGGACATCATGAATTAGTAATGTCAAGTGTGGAAATTTTtGCTTGTTTtACTTTGtGTAATGCAAGATGTCGAGGCCAGTATACAAGGGTGGAGCCAA<br>
 +
GATTTTAGGTAAgCGGGGtCAAAAATTAAACGCATATGCAATATAtATTTTTTCtATTTGCTTATGCAAAGAAATAACTAATTTGAGAGTTTAACTATAACGTAAATATACAATGATGCATGTATTTCTGCTTTATAATTGGT<br>
 +
CCCAATGACTTATTATATCTTGAAAAaAaTCACTTATTCAGATTTTTtACTTATTTAATAAAAAAaGTGAGATTATATtAGCTTCAATTTGTAGTTAATTTATTTTAAAGTACTTGAAAATGTTAAAAAaGGTTTGACCAAAAa<br>
 +
GTTTATTTATTTTCAAGAGTGCTTCTATAAATTCCTTAAGACAGGTAATTAATTAGAAGTTATTCTACAACTTATTTAACCAAATAATTTACACTCTTCTTTTAACTTATGAAAGAGCACCAAGCGTATCTATAAATTATTT<br>
 +
ATAATTTTTAACTTTATACTTCTAAAAaTCATCATTTAAAATTTtAACTTATGAAGGAGCAAACAGCTGGgATTGATTTAAAATCTTCGGGACTAGTAGAGCCGCAAGGCCCGCAAAGACTTGCTTGgTTTTGCATACAGA<br>
 +
TTGCCCATTTGATTTCCTTTTTATGGGCCTTGTAATTTGGTCTGATTTAATCAATAGAAACTGCGTTTGTTCGTCCTAAAAAAaGGTTagATAAAGATCCTCTTATTTGATGATTTCTATTGTGATGCAAAACGAAATTACA<br>
 +
TAGCAAGCTCATGTATGTATTGGAGAGTTCAACACTTTATAGCTTTTTACGATAACTCGTATGTCTGGGCTAGTTTCAGTTCACCTCGAGtAATTTTGGGGACcAGTCGACTGTTTACTAGTGAGAGCTCCAATTAAAGCT<br>
 +
AAAGAAGCTCCGTATGAATTGACTCAAAaGAGTTGAAAGCACCTAAGATGTTTCGATCCTAAAATCTTAAAAGGAAGCAAACCCCTAAGTTTTAGGGTCATCAGGCCAACTGCTTAGGgTTAACTATTTATAGCTTTTAA<br>
 +
CTTGGGGAGGTTGTAATGGTTATTTACAATGATTAAAaGAAAAaTTATGCATAACGACAAAtCCAATAATTAAAAGCTTCATAATCCCTTCTCTCTCTAAAACGAAATCCTCTCACTCTAGAAaCCAAAAACCCTTATTTCT<br>
 +
GTCTTtCCTTTtCCcTTtCCCCcTAGGgCTTCACCCATGCGGCAACAGTGTAAGAACCcGTGTTTTTAAATGATTTTTCAGAAATAATGTTGGGAAAATGTAAGGAAACgAAAATAGATTAAAGTGAGCCAATGTGGTGTTA<br>
 +
ATAGATTGCATGTGAGTGCATTTAGAACACATTAGAGACATGTGGCGTGTGTGTGCAATTAAGGGTGTGCATATACATCTTGAGGGGCAGTTTTTTTTTtAAAaCtAAAaCACTTCTCTCTCTCTATAGGgCCcGCTGCCC<br>
 +
CcTTTCTCTCTCCcTCTCCATTTCTCTTGCCAAATCCATATCCCACACTCCACCTCTTTAAAATCTAACCTTAAATCCTTCATCTATGCGTGATTTGGAGTCCGAGTGTCTTTGGTTTCACTTGGAGgAGGTATATTTtCGG<br>
 +
TTGAAATTCAAGAACAAAAgTTGGGGTTTCATTTCTCACCAATTGGAAGCTAAATCTTTGAGGTAGTGGACAAATTTCTACTCCCTcTAGTTGTATTTTGTTGATTTGTCGCATATACCATTGTGTTGCCCACAAATTtCTA<br>
 +
GTCAATCGTGAAATCTCCCTAGAAATGGGTTTGGTTTTGATTGAGTTTGATTCCCACTTGTTTGGTTGTTAAATTCACAAATTGGTGGTTGATATACCCTTATTCCATGCTTAAATGATATTTGAAATTCCAAAATTGACA<br>
 +
TGGGTTGGAACCCCCCTTCATTTGTGAATtGCTGTTAAAAATCTCATTTTGTAGcAGTAGATACCAAGTTAGTGAGGTACGATACCTTTTGGTTTTGTTCACTGATGGCGCGTTGGTATCTATACCAAGTTGGTGAGATAT<br>
 +
GGATACCTGTTGGTTCTGTTCATTGACGgTGCACTGGCATCCATACCAATTTGGTGAGGTATAGATACCTTTCTTTCTGGTCGCTAAtGGTGCATTGGTATCGATACCACTCTTGGAGGTATCCAtaCCTTTTTAAAAAaGT<br>
 +
TCCAAAAAACTCTCATTTGGTTTCCGGTTGACTTATTTGGTATCATACTTGATATATTCACCCCGTGCACTTTATTTTTGGTCGAATTGCGCCTTTCTAACCTACTTtCATATGATTGGTGATAaTGTTGATCTTCCAACGTA<br>
 +
CTTTGACACATatAAACTTTGTAGAAATTGGCGATCTACGTTACATGAAATGAAAATGTATGATGTTTGTTATTTGGACATGTGTGAAATACTTGGTGATTTttGAACGAAAAaCAAGTTGATGCACTAGGATCACTACTCAG<br>
 +
TGAGTCACTAGGAAAGGTTTCACGAGGCCCATAAGACTCGGACAAGGGTTCTACGAGGCTCATAAGATTCGGATATGAGTTCCACgAGGCGCATAAGACTCGGACATGGGTTAAGACTCGGACAAGGGTTCCACGAGGC<br>
 +
CCATAAGACTCGGATAGAGGTTCTACGAGGCCCaTAAAACTCGGACATGGGTTCCACGAgCCCATAAGACTCGGACATGGATTCCACtAGGCCCAAAAGACTCGGAAGAGAATTTCAATATTCTCAAGCTTGAATTGATGT<br>
 +
ACACAtGAGATTGATAAAATGTGAAAATGGTTGAaCGTGGACATTTATGGGATACGCGGATACTCAGAGACACAGTCGGGTAGTTTACGGgCATGTTGGGTTTTCTGTTATCATATGTGGGTCCTATATGGACATTTGACCAT<br>
 +
ATTTTTACCTTTGGACATTTGTTGGTCATTCGTCACATGTTTCGAATATTtCGTAGTATCCATTGGTTGTATCACACATGATAGTCATTCTCTTATGCACATATCACACTTTATACACCTAACGTTTGTAGAATGGTTTtCCTAA<br>
 +
TGGGCTAGTGAGCTCACCCTATCTTTTTCAGGTACATGGCAAGAAACATGTGTTGGATGCTTGGAGTGTGGATGCATtGCATGAAGCTTGTTTGATCATGAAGTCATTTTATCCTCCCCATACTTAGTATATTTTTGGGAGAT<br>
 +
GATCATGTAACTTGTGAACACCCCGCcGGAGTTCACGAAAATGGTTTAAGAAAGCAGTTTCGATTgATTGGA<br>
 +
 +
Got<br>
 +
[[File:CHI.png]]
 +
 +
 +
 +
5) DFR<br>
 +
 +
I submitted the cDNA from grape<br>
 +
ATTTTCTTCCCCGGATCATTTTTGAGAAGTATGGGTTCACAAAGTGAAACCGTGT<br>
 +
GCGTCACCGGTGCCTCCGGTTTCATCGGTTCATGGCTGGTCATGAGGCTCCTGGAGCGCGGCTATACTGT<br>
 +
TCGGGCCACCGTTCGCGATCCAACTAACGTGAAAAAAGTGAAGCATTTGCTGGACTTGCCCAAAGCGGAG<br>
 +
ACGCATCTGACTCTCTGGAAGGCAGATCTTGCTGATGAAGGAAGTTTCGATGAAGCTATTAAAGGCTGCA<br>
 +
CCGGCGTCTTCCATGTTGCCACACCCATGGATTTTGAATCGAAGGATCCTGAGAATGAAGTGATAAAGCC<br>
 +
AACTATTGAAGGGATGTTGGGCATAATGAAATCGTGTGCTGCCGCAAAGACTGTCAGAAGGCTTGTATTC<br>
 +
ACATCCTCCGCAGGAACTGTGAACATTCAAGAACACCAACTGCCAGTGTACGATGAAAGCTGCTGGAGTG<br>
 +
ATATGGAATTTTGCCGGGCTAAAAAGATGACTGCATGGATGTACTTTGTCTCCAAGACACTGGCTGAGCA<br>
 +
AGCTGCATGGAAGTATGCCAAGGAAAATAACATTGACTTCATCACTATCATACCGACTCTTGTGGTTGGC<br>
 +
CCCTTCATAATGTCATCAATGCCTCCAAGCCTCATAACTGCTCTTTCCCCGATCACTGGAAACGAAGCTC<br>
 +
ATTATTCAATTATACGGCAGGGCCAATTTGTTCACCTGGATGACCTCTGCAATGCTCATATTTACTTGTT<br>
 +
TGAGAATCCTAAGGCAGAGGGACGTTACATTTGCTCCTCCCACGATTGTATCATTCTCGATCTTGCAAAA<br>
 +
ATGCTTAGAGAAAAATACCCCGAGTATAATATCCCCACAGAGTTCAAAGGTGTTGATGAGAACTTGAAGA<br>
 +
GTGTCTGTTTCTCCTCCAAGAAGCTGACAGATTTGGGGTTTGAGTTTAAATACAGCTTAGAGGACATGTT<br>
 +
TACTGGAGCTGTGGACACATGCCGGGCCAAGGGATTGCTTCCCCCTTCACATGAGAAACCTGTAGATGGC<br>
 +
AAGACCTAGAAGAAGAATTTCTGTCAGGCTTTCTAGCGAGAGCGTAGAAATGTCCTTCATTCTGTGGAAT<br>
 +
CAAGGTTTTCTGGTAGTTTGATTTGGCCAACTATGTATGAAGTTTGTCTACTATTTTATCCATTAAAGGA<br>
 +
AATTATTCTAATGGTCAATGTGCCGGAAATCAGAGTTCGTTC<br>
 +
 +
Got back from BLASTn search:<br>
 +
[[File:DFR.png]]
 +
 +
 +
 +
 +
6) F3’ H
 +
submitted from scaffold (near SSR primer)
 +
 +
ATCAGCCAAAGTCACCAGTCACAGACTATCAGCATGCACCCTGGGCCAAGATCAAACTGTCCGTGTGCAATTAAGGTTTGATTATAAAATCCCAACAGTTAAAAACTAAGTAGTCATTACATCACTTtGGCTACTTATATATTCA<br>CCTAGATAGAATCAACCAACAGGTTcGTATGGCGATTCAGAGACACATGAAATACCATTGATGACAGGATATAAACACACCAACACAAATGCCTATtAATACAAATGAAGAGCAAGCATGAATTTTGTGCACATCATAAGT<br>TCATTAGCTAGGgCATAAATGGATACAATCTAAACGAGTCCTCAAAGTGCCGGCATTTtGTCTTGTTTTTGTCATTATTGTAAGAGCTGCTTCCTGCTgATAGTGCATTcTCTCCAAACTAAACCTACTAGGTACAAAACCTT<br>CATGGCTTCTTCAAACCTGGGTGGCTACCAAAGTACTACTCACCATTACACTAACTGTACTGACAACTTTCTTTATCTTGTTAAACCTGCTGAATACTTATCATTTGTAGCTTGTTGCTTATGTTAAAGTTGAGCTTGTTAA<br>CAAGCGGCAAGGCTTGTACCATGATTGGGTTACTATGAGACTAAGAGCACATACACGTCTACCGGCCTCCATGTGACACAAAGAGCAGAGTGACAATGCAAGGAGGTAGTGCATAAATGAAGCTTACATTGGAACTTTGT<br>AAAGTTTTAAACTTCAAAAGATGTGTTTTCTAAGAGTTATGTCTAACTTTTAGTTTTGAATGAAAAAACATGACTTTGCGAAAAGATGTTTAGAATCCTATAAATATAGGATCACCCCACTTACTTAGCCTAAATGACTTTT<br>AATTCTAACAACTCTCCGTATTATTTTCATATATTATGGGcAaTTcGATTCCTTCTTGGCACCCCCGTCTtCCACTGATCGTGTAACCTGGAGACAAACTGCGAGATCTATGGAAGGTATCTTGGAGTCGTATTCAGCCTTTG<br>GAACCTTCATGAAGGGAGGCAAATATGGCTTTAGGATTTATCCAAAAATATGGCTTTAGGACAGcGATTTGTCGTGCCTTTCATCGATTtCACATGCTAGTTTCCTAATTCTATTCATATTACATATTTGTGTGAttGTGTTCA<br>TATTTTTAGTGTTCAATGGCATTTCTAATTTCCTAGTGTTTTGTGATAATCATGTGACAAAaTCCCAACAGCTTATTCCATTCTAAGGCAGTAGTGAAGGAAACGACAAGTCAAAAAaTATGGACACAACCCGACACTCTAC<br>GGACAaCTGTCAAATACACCTAAATATGTGTCTAATTTTTCTGTACTAAATTTTCAAATGAACATGTTGTGGACTCTATAGGAAAGGTtGGGGATTCTCTGGGGCACGTTCAAGACACATAAGGAGTTAAAATGCAACTTTT<br>AAAAGTGGAGTCAAAACTTCCATTGCTGTTGAGTACATTAATATTAATGTAGTAACCATTAATTGGcATAATGTTAATACTATTGGTGTTTTTTGTGTTAATTtGATAGAAATAATCTTTTAACTTTTATACGAATTCCTACG<br>AATCATTGACTGATATAAATATACATTATATATtCCACGTGCCcTAACGTGCCCgAaTCTTAGTTTTTACGGAGAAGACATCCGTGTCCATTTTGTGTCCATATCCTTGCTTCTTAACTGTTAGATAATGACGATcAAAAATT<br>CGATCACTCCAAcGATTGAGACACTGCGTATAACACCAAACTCTCCACCAACTCTCTCACAAGTTGATGTGTCCCACACcTGCGGGGCTTGTGCGAGGGAAATTGAGCTGTTACAGTCTAACTAACGAATGCCAATTCAGT<br>GGGCTAATATTTAAGTTATTGTGTTCGATTTCTATGTTGTACTTCCATAAaGAAAAATTGGTGCATCTGTTGGAGTTTGTCCCACATTGGTTAATTAAAACCTCCAAATCTAGTATATGAaTCTGGGCAGCCTCTCCACTCA<br>TTGCCAATTGGTTTTGAGTTGGATGCATTAACATGGTATCAGAGCTAAGCTTTCgCAGGCTTTGGACCAATCTCTTGATTGATTGCCcTCTTgTtaGTGCGATAAAGTGCACCTTGTTTCAATATAATCCAATGTTACGGATC<br>TTTGTTTACCTCTCCCCATGCGGGAaCTAGACAGGGGTCGCACATGCGGAGAGAGCATCTAGATCTCAAGGCAAGTTGAAGTGAGaaaaaaaaaaagaaCAGTTTGTGATAAGCAGCAGAACTGAGACATCAGATGCTTGCTT<br>AAAAAACACCTTTCGATGTAATAAACAGATGGGCTGCCAAGCATGTGAccTTTTAAAACAGCaGTTAATAACATAGAAATCGAAGGTCTCCTATCTCACTACAATATCTATCCCCTAGACAATCCcCCTAAGTgTCTCTTtCG<br>AGAcaGTTTTCACATTATTATTGTTATTGTtCCACATTGCTTGGGTATGGAATGGGAGATCACTTAATAACATATGGAACCTCTCCACTAATTGCCAATTGgTTTTGAGTTGGATATAAAGTTTCCAAATGGTATTAGAGTGG<br>gCCAATTCCATCCCAAATTTCTTAACAAAAaTTAAATCAAGCCCACACCCCACGATGACAGACCCAGGAAAGGTTGTACgATGATAGAACCCAAAAATTGGCCACAGGTGACAGACCTAAATGTGGCTGCAGGTGAGGGG<br>GGAGTAAGAATAGTTGTCTCATATTGCTTGGGTATGGAATGGGAGATCACTTAATAACATTTGgAaTCTCTCCACTCATAGCCAATTAGTTTTTGAGTTGGATATCCACAATTATTTCAACATTGACTCAACTGAGCACCTT<br>TATCAATCTTTCATATCGACTCCCGTGTACAAGAGGCAAATCAGTGCAAAATCACCATAATACATAAAGCCAAAGCTTTCAAATATTCAACCACACCTGATATAGTCTCAAAGCATTTGAATTTCCAATTAGAGCTTGCAG<br>ATTAGACTTGGGAAGATCCAGCAGTTGTTGCACATTTGAGAAACCCCTTCTGCTTAGGGAACCGAAGAGATCATCAGTCATGCATGGCAACATCCATAGAGCAGTGTCACTGCCAAACCATAAACCCTGCAAATGAATCC<br>AAAAATCCATCAAAGTGTTGCATGAAGAGGAGAAAAATAGCAGAACCCACAAAACTAAACCTTCAAATGGAGGCTGTTTAAATCTTCTTGATGGACAGAATAAAAAGAAAGACAAGTGTAAGAACCAGGACACAGCTCCA<br>GGTTATTTCCAGGAAACTATTACTGCAATGGATAACATGGAACaatttATGTTCGAGAAAGAAAACCAGGTCACCTGTCCTGCAAATAAcTTTAGAAGTAGCCAATTTCCAAGGGACCCCGTGaGAAACCCTCGGCCGTCCG<br>ATGGTGTTTTGGACGGCCCGGATGTGTTAAaCACATTTGGGCCaTCCAAAACACGATtGGACGACCGTCGATAAGAGTGGTACATAAAGAATCGTTTATTCCCACAAGAAGATGCCTATTCAAATGGTCGAATACAGTTCC<br>CTAAAAGAACTTACAACTTACAAACTTTGATAATCATCCCTTAGCTGTGGAAAATAGGTCCGATTCACTTTCTTACCGAAGGGCGGGgAGGATAGATTGATTCAtCTAGCCTGTCAAGTAGAGCCCCCTATAGACGTCAAG<br>ACTTGACGGCCTAAATGGGTGTATCATGCCTTGGTtATTGATCCCAAGGCCCAATCCAGGATGAAAACTCCATCTATAGATCTCTAATGAGgACACAACCTTAACAcGATGGCCAAAAaTAGAAGCTTGATGCGTGAAACT<br>TCTGAAGAAAAAATTAACCCAAAAAGTTGATGTGTTTCGAGAAACATAAAGAAGCAATGGCTAAAATGACAAGATGATATACTGGTAGGTACAATAAACTACAGtGGGTAAATACAACCAACGAAGAGAGCCTCCAAGTTT<br>AGATCTCCCTATTGAATATTGGAGACCAATTCGTTGCTGCCAAGGTCAAAATTTGAAAGAGGCATAAACTAACCTAAGAATAGAACAAAACTTTTTATTTTGACTTCTGGAGCTTTGGAGGTCTAAGATAATCCCAAAGTC<br>CAGCGTGGTTTCTCTGGTCACAGAgaGAAATTTTTCCTAAACgaTACAAGAGATAAAGATGAcAACTATTAACTACCAACAGGGATAAAACCATGGTAAAAATACAAACTATACATAGATTTTTGGGTTATGGTTTACCCAC<br>cATTTTAATAAGTGCAAGTTCAGGAAAAGGTATACATGTATGTACCTACCCATTAATTCAAATTGCTGCACTGATGCGATTAACACAGAAACAGAATGTACTAGAAATCTTTCACAAGGGAAAAAAaTTTGCTTGGCCAGA<br>ATCGACAAAGGCAAGTCTAGTAGTACCAGCAATAACTTGGTCAATACAGAAAAATGCAACTTTTGCATCTTTTCTGAAATAATTGTACTATATTCCGCTTGTTTAGGTCTGTTTCGCTATTGGgTTGTCTTTTTGTGTTCAA<br>TGGAAACATAAAGCTTCATAATtCCACCCGTGGTTCCTTTCGGTCTCTTAGGAAATGGAAAGAGTTCAGCCATTTCTACAGAAACAAATATGTATTAATTTCATACTTCAACGAATGTCGCTTTAACgACATACCACAAATT<br>CACCGATGACTTAATTATTCTGGATTCACAATGACTGACTTAGCATCATATCGAGAAGTGCCTAGCAGTATCTGTGAAAGACCAAACATGTATTCcTGTCCTCATAGATTgttGGTACAAAACTTGAATTGATCGGGAAAGA<br>AGTGCCTGGTtGGAAATCATAGCAAACAAACTATAGTAACAGCCTCAAGTGGTGAAGGCATTCCCAATGTAGATCCTGAAGAaCTTATATCAGACAAATGTTGCATTAACAGTCAAACTACAAAGTTGAACATTTTGTACT<br>TAGTTGCATGATGGACAATATTTTACCTATCATTCGCAATGAAACTGGCAAAATCACTACATGGGCGAAATAAATAATCAACTCTTCTAACTAGTTCAATgTAATTTCAGACATCCAGCGTCCACTGTCCACTCCCAATTTT<br>CTATAGaTATGAATAGGATTATTTCATACGACATGATAAAAGAGGTCATCATAAAAACGACGAAAAACTCCTCATAAGAACATTCGGcAGAATGCAATATATTGTTTAAAAGCAAGCTTTTAACAACAGCAACCATGTGAT<br>TGATGAGACTGATGATTAAGATGGAAAAGACAGTACATACAGAATTAGAATCACCATTCTATTCTTATCTATCAGACCGTTCATCTACTGGGAAAAAGCAAAAAAGCAACGACCGGGAGAAATACCTGCATGACCATTTG<br>AAGAAGATGCATACAGGTGATTGTACTTGAAAGCCATCCACTGTTCGCACATATATCTATCATTGCCTGTATGATACGTATACTTTGATCGAGCACAGATTTCAAGTCTGTGACATAGTCTGTGATtGGTAATTCTAACTGA<br>GAAAAGTGGGCCTGCAGACCAAACATCATATATTTAAGAAGATAAGACCCCAAGAACAAACACAGAACCATGTATTATATCCAGAAGACAGTCAAAAAGTGATGTCGACCTGGAAAAGTAGATTCGCTTTAACATGTGGA<br>TCATCTAAACGATTCTTATCCACCATATACGGAACTTTCACAGACAATGCTTCATTGTAGTTTTCCTAGAACCAAAGAGAGGATGATTAGAGAAAAAGTCAACAGACATTTCATGTGTTATTAATATAAACTTTGAAGATGA<br>AGCCACGACAAACAGCCAATAAATCATATAGCCAATAAAAGAGTGCATCATCTATGTGTCCAAGAATtGGATTCATCGACTTCTCAAGCCCAAAGACTAGGGAACATCAAACATTCAGATTTTTTTAATATCATTCTTTCAT<br>GTGCTCTGGAAAAACTCTCTTCACAAAaCATAGATCATTACGCATAGTTGTTCAGCtcTTTtTTTTTTTtAAAaGCAACAATGTCAAGgAaGTAGCACTGTTCCTGAGTTAAAATATACACCAACACGTTAAACTGAGACAGCG<br>AGAAACCCTtCCTGGTTGGTGAAAGCAAAATCAGAATTTATTAGTTTGATAATTAGCAAAAAAAAAaGATGATCACAATTAGAAAACCACCTCATTATGCCTCACAGGAAGCTCATCATATTCGGAAGAACCAGACAGTAT<br>GTGAAGGAAAACCTGCAACGAAAAACAAATGAAGTGAAACATTGCTTAAAAAGGGATGAAAATGGAAGTTATACCGGCTATATGTTGGAGTTTTTACTTCAAGAGATGTATCCGGGCCTATATTTGAACCAAACATTGAA<br>ACAGTCATGTACTTCAGGTAATACTGAGAAGCTATTGAGCCCAACATCATAGGTTCCACGCTGTCTTCATTCATCTTCAAACACCCACTGTCTTCCAGATCCTCAAAGGTGTTTTGCACCAAGCTGCCAAACAAAACGGTT<br>ATAGAAAATTGCACTACAACAAAACCTTGGGTCATACAGTAGGTTATTACCTTGACAAATATGAACTCACAATTTCAGGTCCAGTATCCtCTAAGCCATAGTAAGCTGGATTAACCATCTGTCACAGGGATATATTTTTTGT<br>AAGAAACTTTAGCAGGGAAACAAAGACAGCTGAAAGCCCAaTGCTGGGTATAGAAGCTGTGGTGTGATTCCATCCTCAGTCGATCATTGTTCCAAAGATGAAAATATCATAAACATGCAcAATTGAACAcTATAAATGCAT<br>CTAGTCCATTATTTCAGGGAACATGACatGCATGTTTTGGGCAATCACTACAATCATTACGCTGAAAATGCAAATTGAGAAtAGCAGGTATCCAGGTCAACTTCGTGCTTATTATGAGGCATACATGGCATCATTATTTTGC<br>TAATAATGTTAaGCAAGAGAGTGTCTCTACGCATTGCTTAAGTTGAGACACCAAATCGAGCCTTCCACATCACcGAACTCGATAATCATTATAGACTAACAGAGTTTTAGTAATTCACTCCATACGCTCTGCTTGTTATCAT<br>CTACATAATTATATTAATGGGTTTGATGCCTTAAATAAATCTTTCCAAACAGCCGGGAAATAACCAATATTTTACTAGATTTTAATCTCAACTTCCAAGGGTACAAAACATTTAAAAAATATTTGGTATTGTACATGACCAA<br>ATAAGTACAAAACAGTTTCAAAAACTTTTCTGTTGTTTTCTCAAAATTGGGTTGTCTTTAGAGAGAAATATTGTTTGCCAAACAAGATTGGTGTTTAATGGAAACGGAAAATAGAAAAGCAAAACAAaCaaaacaGGTGATTA<br>GCTACCATAAGTTACAACTACTGTTCAACTTCAAAGAATTTAGCATTTCTGTTTAAATTAAAAGAATCTAATGTTAATCATGTGAAGAAACCAAAGAAGCaCTTCCAACAGCTAAACTAACACGGAAAGTTAACAACCTGG<br>TCATATGCATTTTACATTTTCCCCATTTTACAATGTGCTCCCACAAAAATGTGAAAACGTGTTCTTATGTAACCTCTCTTCAAGTTTCGGATACAGGACTCTGCTTGTTGACATCAACCTTGCATAGATGTTCAAAATATTT<br>TCTGCCATTATAAGACAAAAGAAGACCATTTTTTCTCTTCCCAAACTTTTGTATGCATCTTCCCAATATTTAAAATGTGCCTCACATCCCAAGTGTAAGTGTGTGTGTGTTTAACACTGGATGTtCTTTCCTGATAGGTGTAA<br>GTGAATGTAATCAACATAAATATAGTTTGACAaCCGAGAGAATGTCCAATATATCTATAGAACACAAAACAAGAACTCCAGTCTCtAGTACACGCTTTATCCTTCTGAAATGAGTATAATAAGAAAATGAGGGgAAGCCTA<br>GAATAAGTCAGGACCAAGCAAGGTGCTTTAAGCgCTGATTTACTTATTGCGCATAAAAAGGAATTGCcGAAATTTTACCAATCTCCGAAACAAATAAGTCCAGGTGAGATAATGAATTGCATCCTCTCTATGGCAAATTGT<br>TCCAGAAACTATCTCAGCATTAATGTGGTCATGCAACTGCTCCCTCAGACTACTTTCAACTGGGAATGGTTCATACAGAAACTGCAACAAATAAAAC<br>
 +
 +
 +
[[File:F3_H.png]]                                                                       
 +
 +
 +
7) FLS <br>
 +
submitted from scaffold near overlap sequence with query<br>
 +
GAGAGAGAGGgaGgTTGcTGCAATCACGCTCTAGTATCGGTtGTGAACTCTGCCGCAGGAGAGGTGCGAATGGTAGGTGACGTCagCGGCGATGCGaTGCTGGACGCcGGTGATGGGACTGGGAGGGGCTTGGGGTTTTGAAGGACCAAAGGGAGTAGACCATTAGGAGAGTtAGGTGGAGGTCC<br>AAGAGTGTCGTCGTTTGGGTCTGGTGGGTTGTTTCGCCATGGAGAGGTGGTGGTGGTTGGCAGTGAGAATGGGAGAGCTCAGGCGACATTATTTTTGAAGGATGTTTtAGAGGTGGTGGTTATtAATATgTGGgTTATGTAGAGGAGTGGGgACGGATACGACACAACACTCTCCGGACACG<br>TGTTGCTTAAATACTTGTCtAATTTTTtCTTGTATTTCTTTTATTCAAACACATGGTAGATGTTCAAGGACACGTTGGCAAAACTCATAGAGCGTGCTAAGGACACATAAAAGGTTAAAATaTAATTATTAAAACTtGGCCATAGGTGAAAATTtCCACCGATGTATAGTCATGTATACTTAtgCg<br>CAGACTGCTAATTTATCTTTGTTGtaTTaTTTCTAAATTAGATAGAAATAAAGATTTCTAATTTATATGAATTAATCACATACCTCTATGATACAAaTAAaTTTtATATTGGACGTATCCCCAACGTGTCCTTGTCCTAATTTTtGAAGAAATAACGTGTGTGCGTATCCGCATCGTGtcGTGTTCG<br>TATCCCgTATCCGTGtCcGTGCTTCTTAGACAATAAATATTGATCgTCGGTAGCCACACAGTTTACTAAGGGGCAAAATGTCTAACTAGTTGGATTCAaCTATAGCAGTAGCCGCTAAAACTCTGTAGGCTCgCTCTCTACTCCTATGGTAGCCTATGCTGCTAACTCAGTTGAtGGaTAGCAT<br>GGATCCcATAGAGCTCTCTCAGTCTCTAGCCTTGTTTATGGCTCAAATCCCTCCTCATCTCCAGTTTCACATCACCGGACAGCTATATAGCCAAGCCTATTGTATTTTTtGTATGAACACTATGGAATCTTTCATTCCgAaTTCAACACGGCTATCCAGACGGAACCTGAAGTGAGGCTTTAAT<br>CTGGGTTCTCCCATACGTACCCTTTTtATTGAGAATGCAAGGCATTTACGGATTGACATCCAAAATAAGATaCAGACATGGGACAAAGCTATGTGAATGCCGTGTtAATTAATTGGGGAGCTATTCCATTAGTATTAATTTTGTATACCACAGAAACTTCTTGCCTCTAGTtGTCCACTTTTCCC<br>TctcATCCTCTCATTCTGGGTGACCCTTATATGTGCATATATATAGATATCAACCCACAAACGCAAGGTTGATTAGTGATCATAGAAaGAAAAAaTGATGGACCAGAAGGAGAGAGTGCAGGCCATTGCCTCGGTGTCAACAGACACCATACCGGCAGAGTTCATCAGGCCAGAAAGTGAGC<br>AGCCCGGACTCACAaCCTaTGGTGGGCAGGCCcTTCAAGTCCcGGTCATCGATTTCTCAGACCCGCACGAGGAAATCCTCGTGGGTTCCATTGTCGATGCAAGTTGCcAaTGGGGAATGTTCCAAATTGTGAACCATGAGATTCCAGTAGAAGTAATAAGAAAATTGCAGCAAGTTGGgAAA<br>GAGTTTTTTGAGCTCCCACAAGAAGAGAAGGAGGCTTATGCTAAGCCTGTTGGGTCAAAGAGTGTGGAAGGGTATGGAACAAAGCTGCAAAAGGAAGTGGAAGGGAAGAAAGGTTGGGTTGATCATTTGTTCCATAAGATATGGCCTCcTCATACTATTAACTACCAATTTtGGCCTAAAAa<br>CCcACcTTCTTACAGGTTCgTGATaCATTTtCTTCTCTCTCGATTATTCgTTAGGGgCAGATAGAAGGgCAAACAaTTAGAGTACTACTGTTTTGgCAAAAAaaTGGATtATTTTTTTGTCTTTATTCAAaTAAAATTTGCATTtgTtAGTTTTGCGTTGATTTTTTGTGGATTATTagTTCATCTCGA<br>CAAGATGAATCTAAAAAGTCAAGAGTTACTATCAAAattaaaaaaaaaaaGTTCaCTAAAGACAATAATtCAAAATTTtCTCAAAaCACACATTCATGCGATTCGACATTtGTCATTACGCATGCGTATaTTGGGAaCATcTGATCTGCaTTTtttATTttGgTtATTTTtCTTGGTGTcTTATAAAAATGTT<br>GtGTCTtGTt<br>
 +
 +
[[File:CV091044.png]]                                                                     
 +
 +
 +
 +
8) LDOX
 +
submitted from scaffold
 +
AAGCTTCGTTTAATTTTTCAATATATAAGAAATGCCAAAAAATGGTACATTGAGACAGTGGAGAGGGAGAGAAATCCAACCAAAAGTTAAATAAGAAAAAATTAAAAAACCACCCAAACATTAGACACCCGAGTACCCAACGGCCTCAACCTTATCGCCTTTATCGGTGAGGTAG<br>AGACCGTGTCAATGCCACCGCTGCAACCCACCACCAACAATCGTTTTAAGTTGCATCCAATTATGTTTATTTCCATAAACTAGCATAAACATTTTTCAATTTTTAGTCTACCTTTTAGGTATGGAATTTTTCATGATGTTATCTTGAAGTTTGGCTCTACCGACTGACTAAAT<br>TTTCGTGGCTCCGCCCGCGCGCAGGTGCCAACCATTGATCTAGAAGACTTGGTATCGGAGGACAAAGAGGCCAGAGAGAGATGCCACGAGGCGTTGAAGAAGGCGGCGACAGAGTGGGGGGTGATGCACCTAGTGAACCACGGCGTCCCCGAAGAGCTGATGGACCGG<br>GTTAGGGTGGCCGGAGAGGGGTTCTTCAATCAGCCCGTGGAGGAGAAGGAGAAGTATGCTAATGACCATGATACTGGGAATTCTGGGAAGATACAGGGGTATGGGAGTAAACTGGCAAACAATGCCAGTGGGCAGCTGGAGTGGGAGGACTATTTCTTCCACACTGTTTA<br>CCCTGAGGACAAGAGGGACATGAAGATTTGGCCCAAGAACCCATCCGACTACATGTAAGTTTTTTCTGAATAACGTCCATAGATTTGCTGTTTACATTAACGGGTCCGAACATGATGTATTTAGAACCATTCTACTCCGATAAACCTGGTGGTATAGAAAGGGGCGTACAA<br>AAAATGTACACAGCGCGTTTGGGGACCCATTTTTGGGTTCCACATGAGTGATCCGAACCGTTCATTGTTCTAAAACATTTTTCTGAGGGGGATCAAACAAATCAGCTCAATCGGACGGTTAGAAGTGCTTGAACCAAAGTCCCACCGTTTCAAATAAAAACTTTTCATTTGA<br>AACGGTGGGACTTTGGTTCAAGTACTTATTATAACCATCCGATTGAGCTGATTTTTTATGGGCCCCCTCGGAAAAATGTTTTAAGAACAATGAACGGTTCGGATCACTCGTGTAGGACCCAAAAGTGGGTCCCCAAATGCGCTGTTTACATTTTCTGTACACCTCTTTCTGT<br>ACCGCCAGCAGCGTCCGTTCTATTCAAGTAGGAACTACAAAAATTTATTCCACTAAGACCGCACATATCTCGAGTAATTTTGAACTTAATTTGCAGTACAATTTACTAATGTTTTATGCACCG<br>
 +
 +
[[File:LDOX_EST.png]]         
 +
 +
 +
9) MybA1<br>
 +
did tBLASTn using amino acid results from scaffold03500<br>
 +
LGVRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG<br>
 +
 +
[[File:Myba1_EST.png]]
 +
 +
10) MYCA1 <br>
 +
submitted from scaffold 00278 (BLASTn) <br>
 +
tacattattttcctttctttctttCTCCTTTCTGACTTGATTGACaTAGTATTAACCGATACGATAGTATAATAGAaCAAGGGATCTACCCTGTTCCCTTCTCCTTTACGGTTCAGgAATTCATCTCCATTAGTCATGAACCCCGcTTGgCCcGCCATTGAGTCACAACTCTTCTtGTACAAACTTC<br>TTTCAACTgaTCAGTCGATAGGAAAGTCGGATGACTCAGATGCCTCTACTGTAAGTaGTGGGAggtGCATAATtCATTATACGGGGGaCCAAATCACAATTTtgAGGGAACATTCCTTCATAATTtATACAAACAAAACCTAAAATATAGTACCAATAATGAATAATTGGGAGg<br>TGTGCCTACCCGGGCCCTCGTATCCACCCTACCTGcAGGCTGAAATATTTCCTTTGAATATGTTGATGTAGTTCcCCTCAATTTTTTATTTTTATTTTTTAGAAATATCACCGTTTGGAAAATCTCAAATAAAgAGTAGTGGGATATAAAATAAGGAATCATTTCCTCTCCATA<br>CTCAATCCCTCCATgTCTTTCCTTACTCTTAATATTCATTCATATTtCAGGGCCAAAACAATGATCCTAGCcGTTCACATCGTAGAACACaTAGAAAAATATAGGTTTGCAAAATATCAATATGATCATCCATGATAAATTTGTGAATAAGTTACCTTTTGTTTCGTAGATTGT<br>AAGGTTAAACTGTAGAATATATTTGCCATTCATTTTGCATAACAATTTGTAACATGTTTACATTGTCATTTAAATTTGACTTTATATTTGAAACTTTATATTATTTTAATTAGTTAATTAATTTGTCTTAACAATGAATAATAAAAAAAaaTTcTAAAaGTGAaTAATATTACAAT<br>CACGTTTAAAaGTGAAAAAaTaTTACAATCAACGTTGGCAATGTTCAGCCCTAATCAATATTGCCTCCAAATTGTGGTCCCGGCACAaCGCCCAGTACGTGGAACCACAAATGAAGGCGCGTGTCATAACTTAAAGCCCATCCTTTCACTTCATCCGACACATGCTCCCACC<br>ActATATTGGGATGTGTGGTACAaAGTGATGAGGGTGCAAAAAAAGTTGCACGAAATGATTGCTAATACGGGCAATAGCTATTTCTTTGCTCTGTTGGACTGGAACTGGAaGGgATCTCAaGGgCAAGTGAGTATAACAACCTCCAACATTCACGTCAAATTTTaCAGCACAA<br>AGTTGTAACGaGATGCAATGACTACCgCcAAACTTATACAGTCCATCCAATTTGATTCTGGAGCTGTAGGTAGGAAATAACtcAGTGACAAGATATTAAaTCATTAAAAAaGAGAAACAAGATATTAGAGCATATTAAAAAGATAAAaGAGAAAaaaaTATtAATAAAAaaGaTA<br>AATAAATAATtATATAAAAGAAaTGAATTAAATAAAaGAATTGAATTAAAtAATTAAATGAATtAGTAaTATAAaGAAATtAATTAATATAAATAATATAAATAAAGgAGGGAAGAGGGgTAAAaGGGTAAAAGGACAACATAAAGGTGGTGGGACAACATAAAGCTTAACTGA<br>ATCAAGAATTTGACTGTTCTATGTTCAaaaAAAAAAAaaaGGgAaTTTGACTGTTCTAGGTAAAAATAaTGTCTTTAAGAGCTCCCAAACAAGAGAACTTATTATGAAGCTGATAAATAGTTGAATTTGCAGTCTGCCATTGCAATTCCTCCTCAACCCACCaTAAAAGCAAGT<br>GGGTCTTCTTCTTTTGAGCTTGAGAGCAGGATCTGATTGCAGAAATGGAAGATTAAGAAGAAACGCATCAAATCCCAGAGTGAGTTTCTTATGTGCTCTTTAACAAGATAGTCTTCTCTGAATAATCTACTTTCTTCCTATTTACTTCCCCCCCcAAAAAAAaTCTACTTTCT<br>TCCCTCCAAATGACTTTTTCTTGGTTACAGCCTTTTCCCCTTTTCACTGTGGTTGATGGCTTTTTCTAACCTCAACATGACAACATCAGGTGCTTATGAAAACCCTGATCTTGATTTTTTATCTTATACAAAAGCTGAACTAAAGCTACTTCAGAAATCCTAAAGTTCTCCTTT<br>AGAATGGCTTTTCTAGTTTTTGCTTTGCATCTTTTGACTAACTGAGTAATGGGTTTTCTTAAAATTTGGTGACtaGAGTTTTGAGGGTTATGTGTTGGGgAATGGCTAAAGGGGCACAAAACCAACAAGGGGTGCCAGAAAATCTGaGAAGGCAGCTTGCTGTTGCTGTTAGG<br>AGTGTGCAGTGGAGCTATGGAATTTTCTGGTCATTGTCAACCAAACAAGAAGGGTATGGTTTACCTTTTTACTATTTTATTTATCTTCATATTTTTATGGTATGTCCTTAAACATTCTATTTCTTCAGTTTCTGTTGCTGGTTTTGCATAAATTTCCAGATCTGGGAAGAATAA<br>CGTCTAGTTATGCTCATTATAAATGCTTCCCATTCCATAAAAAAaTTGATTTCCCTGCTCATTTATGGCCTCTTTCCTGCAAATGTTAATACGCCTGCTGATTTATGGCCTCTTTCCCTGCCCAATATTTATGTTCATgTTTGCCTAGTATGTCATTACATAATTACATTTTCTA<br>TTTCTTCAGTTCATGTTGCTGTTTTCTTCATTATTTTTTGATAAAATAAAATAAAACAAAATTTCGTTGagAAAGCGACGAACGAGACCAAAGCTTCCCTTACAAAGGGTGAAATGCTGAAAGGGAAgAGATTGAAAGAAAAGTAGCTTCAAAGTTCAAACCTAACACAGGA<br>TAATGCTTTtCTAGGGAAATCAACAATTTTCATGGGAAAGCCATTGCTTATAGCAAAGTACACCTACATGCCTTGGTACATTGAAATTCCTCATGCTTAACTGATAAAaTATGTTCCTTGTTATTTTGTTAAGGGTATTGGAGTGGGGTGATGGGTACTACAATGGGGACATA<br>AAGACGAGGAAGACAGTGCCGGCCATGGAACTTAAACCAGATAAAATGGGTTTGCAGAGGAGTGAGCAACTGAGACAGCTTTACGAGTGTCTCTTAGAGGGTGAAGAAAGTGATCAACAAGTGCCTTCTGCCGCACTGTCTCCAGATGATCTCACAGATGCAGAGTGGTA<br>TTACTTGGTTTGCATGTCCTTTGTGTTCAACCCCGGCCAAGGGTACAAGCTTTCTTCTGGACATTTTCATCTTTCTCTGTGgCTGTGAAAACTTaGTTACACTGAAAACTAAACAGAAAAAACCGAGATGTGACCGTTCAAATCTAAACAGAAAACTTAAAGTTTTAGAGTTT<br>CTGAACTCTGCAGAAATTCCTGTGTCCATTTTTGTTtATGAACAAGAAGTATAGTAACTTGCAAATATtATGGAGTAACCGATATTGGATCATTTGTGTGTGTTCATTAAGATAATGATCAGTTTTTGAGGAAATTGCTGTGTAGTTTGCCAGgAaGGGCATTGGCAAATGGTC<br>AAGCTATCTGGTTATGCAATGCTCACAGTGCTGATAGCAAAGTGTTTtCTCGctCTCTTCTAGCAAAGGTCcGTTCTTCTGGGGCTGTGCTCCGTTTTAGATTTCGTGCCAATTTTAGCTTGTTTACTAGACAAGTTTtACGATATtCAGCTCAATATACTCTCTACTAGTTAAC<br>TAACAGTGATGCTATAATTTTCTTCCTTTCTCAAATGGTTGGACTTGCGCACGAATCAACCTCAGAGTGCATCTATTCAGGTAAGCCGTTATTTTTATTAATTGGTACGCAAGTTTGCAAGCACACACATTCACAGGCACACGCACATAAGGACGTTGGTGGCCCCATATAA<br>TAGATCAAACAAGCAGTGAAGAATTTGTTTCCGTGGAGAACGTTTTTTTAGTAATTTTACTTGCAAAAGCTTTTCATAAACGAATCTTGTTGCATATACCGGTAGTCTACTACAATGACCATTGTTTtATTGCAGACTGTAATTTGTTTTCCCTATTTGGGTGGAGTCATTGAG<br>CTAGGCGTGACGGAGCTGGTAATTTCTGTTCTATTGCTGGATTTACCACTATTTTTGTATTGAAGATGCATCAGATGCTATACGAATTATCCCAGAAGTAACTGGTTCTGCTATTCTTTTGTTTTCcTAAGGTATTGGAAGACCCTAGTCTCATTCAGCACATCAAGACTTCT<br>CTATTAGAGTTTTCAAAGCCCGTTTGCTCTGAGAAATCTTCTTCTGGTCCTCCCCACAGAGACGATGACGCTGACTTCATGTATGCCAAGGTTGATCGTGAGCTGGTAAATACAATTTCGTTGGAGAGTTTCTATTCCCCAACTGAAGATATGAGATTTGATCGGGAGGGAC<br>TAAATGACTTTGAAAAAAATATCCAGGCCGATTTCACTATGGGTTCTCCTGACGAATGTTCGAATGGGTGTGAACACAATCACCAGACAGAAGACTCCTTCCTCCTTGATAATGTAACTTCTCAAATGGAAGATGATTTTAGCAACTGCGTTCAAGTTTCCATGAATTCTAG<br>TGATTGCATATCTCAGGTCgTTGTGAACCAAGAAAAGTTTATTTCTCCTCCCAAGGGTGACAATAGTAAATTGAAAGAACTTCAAGAAGGGAATGATACAAAATTTGGATCCTTGGACATTGGAACTGATGATGACTTGCACTACAAAAGAACTCTATCTGCTGTTCTGATA<br>AGAAATTCTCATAGGTTACTTGATAACCTTTGCTTCCATAGGTGGGATTatAAGTCTAGCTTTGTGAGTTGGAAGAGAGGAGAAATCAGAAATGGCCATAGACCACAGGTGCCGCAGCAGATGTTAAAGAAGATATTAGTTGATGTCCCTcTAATGCATtGTTTCTCACTCAA<br>GTCTCAACAAGGAAAtgTGGGGAAATTTtGGTTGTTAAAACAAGAAAGTTATGTGCAACATGATTTGCCGGATGATGGAATAGAGAATGAGAAGTTTTtGGtCCTCAAATCGATGGTTCCTCCTTCTGTTAGCAAGGTAATTCAACTGCCATCTATTGGAAAATATGATCGAA<br>TGACTCAGAATTAAATATTTCTTTACACTTCTTGCTCTCAAGACTTGCTCATTTCTATGTAGAAAATCAGGATGGCAGAAGGCATTGACTTAATCCCCACCCTCAAAACAGAAGGATTCGAATTTTCTGAAAAACAACTTTTCGATGTtGCTAATTTTAGTCACTAAAAGTAG<br>ATTTTCAAGAATAACATAATTTTCTTGGGGTGATGGTATACTATATTTGaGTCCTCAGCATGTCACATTACCTTCACTAATCAGAGGGAACATATAAACAACATGGAAAAACGCACAGCAAAAAAGTAGGAAACGGGAAAATCCTAATCTTATTCATCATGCTTTGCAGGTT<br>GACAAAGCAGCAATACTTGGTGACACTATAGAATACTTGAAAGAACTTGAGGGAAGGGTAGGAGAACTTGAATCTTGCATAGATTTAGCGGAGCATGAAGCTAGAAGAAAcTACCCAGATGAAGTAGAGCAGAAATCTGAAAACAATGAAAACAGAAGGATTGGAAATAG<br>CAATAAGCCTTGGATAAACAAGAGGAAGGCCTGTGATATTGATGAAACAGACCCTGAGCTCAGCAGGGTTGTTCCTAAAGATGGGCCACTGTTTGATATGAAAGTAAaGATAAAAGAGCAGGAAGTCCTGATTGAGATGAGATGCCCTTCcAGGGAaTATTTGTtGCTTGAT<br>ATCATTGAAGCTCTGAaCAaTCTcAACTTAGACGCTCACTCTGTTCAGTCaTCAaCCaTTGACGGCATTCtCACTGTAACCCTTcAGTCTAAGGTCTACTTCTTTCCTAaATtGTTGCaTATTTAGGtCTAAATTTGTTGAAGATATAATTAATAAAGTTGCCCcTCTCTCTAACA<br>GCTTAAGCTTTTAGATAAGTTGGTGGTTAACAATTCAATaTGGTATCAGAGCAGGCAATAGATCCTGTgTTCGAATCTCACTGCCtaCCTGATAATTAAATACTTGCGTGTGTTTAGCCCGCTTATGGGGgAgAGCATGGCTACACGTgAGGGGTCgTGTTGAaGATATAAAT<br>AATGAAGTTGTCCCTCTCTTTAACAAcTTAAGCTTTTAGATGAGTTGGTGGTTAACAATTCAATAAAATTTTAGAGACATTAATCACTTTCCTATTTCATAGTTTTGGAAAAAaTGTACTTATACTAGAAATTTTGTGCTACATATGCACTTTCATCGTTTCTGAGATGCTAGT<br>GATTCTTTAAAAACTTGATTTCAGTTTCGAGGAGCAGCAGTTGCATCAGCACGGATGATCAAACAAGCGCTTTCAACTGTTGCCGGAAAGTGTTGATAAACTCAAGTTTGCATATTAATGACTTCTGTCTTAGGATTTGCTTCAAAACGGTGTTGTGTAATATGTATCATTG<br>CAGTCCTTTCAGTCTTTGAGTTTTCTGGTCTCTTAACAATCACTTCTCATGGTTTACTGTCTAACCTAGGAGCATAAATGTGGATGTAATTTGGCTACTATCTGCACCATGTTTCAGTTTCATGTCAATGAACTACTTCGATTTTCAGCCTTGCTAGTATTTCAATCTACTCTG<br>GCTGATGCAATAGGAGTTCTTTGGCACATGAAGAAAGGGAAATGGTTAAGGAGAGCTAAATCTATTATGATTGTAGTTTTACAATTATGACTTCAGTTCAAACAGTTACTGAGTCTCCATTAAATTGAGATGGTAATTAACACCCTGGGGATGATGCAAGCTGGTAGTCACC<br>TGCCAACCATGGGTCTCAGGTAGTCATGGGTTTGACGTCCCCAAGTACTAGACGGCGAAGTTAGTTCTTCAAATTAGTCGGCCCACTGAGCCGCATATCGAGTTATAAAACAAATTGAGATGGTAATTTGAGTTGATAACCAATGCCTAAATCTGTCCTCAAGATTCTGAG<br>CATTAAGACGCAACTTTGTACTGTTAGCAAGATTTGGCATGCATTAGGAGTTAAACATTCAAGTTGCTTTCCACACCCCCTACCTCTTTCTGAATTAACTATTTGATCCATCATTTACTAACTATTTTAACCTTTGGGAGTTCTTGAAATTAATACCCGAGTTTCCACTATTG<br>ACTAAACGGATTTTCGGTATTTGCTTCAAAAaAaTTCACTGTACAAGACAAGTCATTTCTCCTAACTAAATGGACTAAATGGTGTTTGTAGTGGGAACGAGTCGTCTTTTGTCATCAGGCTGAAGATAAAAAATATATATATATAGCGTGAACAAATCACCCTTAGTTGGGAA<br>ATCTTTAAGCTGAAAAAaAACTGCATCATGCATAACAGGTGCAAACAGCTTATTGTGATTTCTACTAATTGTTCAAGCCATTATAGTGTCTTCATAAAAGGGCACCAACTTTTCATCTTGTCCACCAAAGCTATTAGCATGTGATATGTGCTTAAAGTTCCACAGCATAGCTG<br>TGTTTGGCTAATCCCTTGCAGGGACATGTTTTCATGCATTTTACACAGATTAAAGTGGGCAACGTACCTTATTAGGGACTAAAAAAaGAAAaTGTTGCTACCTATATATAGTAGATTAAATCAAAAGTGGGAACATACCTTATTAGACATGATTATGATGGAATTTGGAATTG<br>CACTGTACCAAAGTTTTGTTTCCAACTACTACCAACCGGTCAAAGCCTCCCAGTCTCAATTGCTGCATTTTtCAAATTACTtGTTTtCCCCATCAATTACCCACATACATATAACTCACCACATATTCACTCAATTGTGTTTGGATCATGAATTtCCGGCAAAATATGAAAAGC<br>GAAGAAAATAACGAAGTGAATAAGCTAAATTTATTTCGTCAACTACCACTATTATACCTTTCGTAGATCCATAATCCAAACACAGCCAAAGTATGACAACCCAGTGATTCCAAGAAACCACAAAAGAGTCTTATTCTACAACAGATGATTGATAAGAACTTCTTCTACCATG<br>AACAATGGCTTCTATTTACATCCACTACATTTGAAATCTAATACCAGCAAAATCTCTgACAGTAAAGCCAATTTTCTCAAACTTTCCCTTCTCATTGCTCTCTCTGAATCTCAAATAATCCGAGCAAACGAATGGCTTGTAAATCCTCGAATTTCCCTCTCCTACCACTTCAT<br>CAGGTGCCAAGATCACCTTATCATTCTCAAAGCACCAAAAGAAAGCAAGCGAAAACCGGTTCAGTGGTTTTTtCAAAACAACTCTGTGTTCTGATGACCTTAACTTCTCATTGCTCCAAGCTTGAAGCAAATCACCAATATTGACcACAAGGGTCCCCTCACATGGACTAaT<br>GTCAATCCAATGGCCTTGATTTGATCTCACCTGGAGCCCACCCATTTCATCTTGATACAAAATTGTTAGACAACTCATATCAGTATGCATCCCAAGACCCTCAACCTCTTCTTCCAAACTCTTTGGAGCTGAGTAGTTAATTATCCTCAAATAACCATGGCAGTTTTTGTACT<br>CAGAATCATAATACTTTCTTTCAACATCATCACCCAAGGTCATCAACACAATCTTCACAATTTTCTTCGATAAATCTGTCATTGCGCTTCCATATtGTCGCAATATTTCACTAACCAAAAGAACAAAAAAaGGAAGAAAGAAAACTGTCAACGAAATTTGAACGGAAACAGAT<br>GAAGGGATGTTAGGTTGGACTTTTCAATTTATGCACAAAGTGTCTGCTCTTTGAAACTTTTACCACTGAAATAGCAGCCCCTTGTACCCTTCAAATATTGAGGGACTCAAGGGACGTTAGGTTGGACTTTTCAGAAGCTAAGAAATATAATTGACGAGCTGAAAAAACTTCG<br>CTGAAGACGAAAAGATTCTGAACAATTTTGTACTTAAACATCCCCAAAGGAGTTTTAGCCAGATAAGGTGAGTACGTAGATAGACAATAGCATTACCTGAACTCAGAATAGTGCTTGTCAAAGAGAACATCTGCTGAACTCTGAGCAGAGCCGGTGAAGTCTGGTCCAGAA<br>ACTCGGAGGCTCTCAAAAAAaGGAGATGCTATGTAATGAGGAGTGTAGGTTTTGATAGACGAGAAAGGACCGAGTTTGATTTtCGTCTCATCAGGAAGACTGAAGAGGAATTCTGAAAGTAAGCGGATTTTATCATAAAGGTCTTTCGAAATCCCATGATTGGTGATGTGGA<br>AGAAACCCAACTCTTTGCAGGCTTCAGAAaGAGAGGACAAAGAACTAGAACATGGCTGCAATGGCTGAGAGATGTCTAaTATGGGtAGTTGTATGCacaCCTTGGACACCATTTTctATATACAGGAAAGGCTTGGTTTgTCCAtcTAGGTTGGGGCTATTATATATAAATCAA<br>CTGATAAAACAACTTTtGACACTACCATAATATtATtATACAGAGGCCAGAGTAtGGTAGAAaTAGAGATTCACTGTATagCATAGTTGCTTAGGAAGTCTTtGTAAaCAAACTTTCATGAGACTTGTGAGATTAAACATTAAAGGCGTAATTATCTTCGCTTGCCATAAAATGG<br>TATAATAGTAGCCAATTTTTCGCCATTTGTACCTCAACCGTTCcTACTTTCGTTTtCAACTAGCGTACATTCAAAGAGGAAAGAAGAGGGGAAAGGCATCCCATTATTGGTGATGTGGAAGAAACCCAATTCTTTGCAGGCTTCAGAAAGAGAGGACAAAGAACTAGAAGAT<br>GGTTACAATGGCTGAGAGATGTCTATTATGGGTTAgTTGTATGCATGCCTTGGACAACATTTtCCTATGTACaGAAAACGCTTGGTTtGTCCACCTAGGTTtGGgCCATCATATATCCTAtATGGCTATTCTAGTCTTTtGAAAATGAAAGAGagaTATATGAaTGTcTATGAATC<br>AACTGTCCATTTAACCCTcTTTTTtAGAACCctATGATCATTCAAATACtGTCATCAAGCATGTACAGTTCagTAACTCCCAAACTACCATTAAAACTATAAGGACCTTTAGGTAGTGTAACCGACAAGGATTGAGCTCTCTTTCGATGCTCTCAGATTGATCTAGTgaGGAATA<br>GAATTGAGTAtGATGTACATTTCATATGTGCTATcGATATCCTCATCATATATGCAAAAAATACTGtATGTACGACGATAATGTTGtACGTTGATGTCTATtGAGCTTTTACCaTCATAAGAGTAcAATTTTACATGAAATATCCTGTGATAAACAACTTTTGACAGTACCATAA<br>TATTATTAATAGAGGtCAGAGTATGGTAGAATAGGGATTCACCGTATAGCATAGTTGCTTAGGAATTCTTTGCAAACAAAACTTTCATAAGAcTtGTGAGACTGAACATCAAAGGCGTAATTGTCTTCACGTGCGATAACAAGGGTATAAAAGTaGCCAAaTTTtCGCTGTTTG<br>TACCtcAACCTTTTCTACTTtCCTTTtCAACTGGTgtACATTCAAAGCGGAAAAAAGaGAGGAAAGGCATCCAATGCAAAATaCCCGAAGTTATCaTTTTTCAagCAATGCACAGctCTAAACTGAAGTATTAACTTTGCCTAGTCCATGTCAAAACATGAACCAACCCAACACC<br>CCTtATtcGGTGCAGCTTAGGTAATTATTGAGCCCAGAAGAAAGCTAaTGGACGTTAGAGTCCCCCACAAAAAGGCAGATAAGGGGGGAAAAAGAAAACAACATTGGAGACTAAaCAAATCTCAATGGGTAACACTCTTATTAGACAAAACTGCTGTAAAAAGTTCATTAAT<br>GAGTTCTGGAACATTtACACACCAACGCCACAACAACCCGAATTACCCGGTTTTCTAATGGAAACAGACAGAGTAATGATTTGATGCATTTTTtGAtGAAAAaTtCTACAATCAGCCCAATGGGCTATCGTTAAGGTTTTAAAAGTGGGGACTTTTCCGCGTAAGAATCTTCA<br>AAAAGTGGGAACTCTTGCACGCAAGAGTTAACTCTTTCATGCAAAGGTCTTACCTTATTGTCAATCTAGTGGGGACAGAAGGATTGTTTTTTTtATTtGCGGCCACTACACGTTACTCGGCCAAATaTATAGGAAAATATGACCTGCTCCTTTTCTAAATGCAGGGCACATTT<br>TTtGTTCTGAAAAGGAATCTGcGGAGGTAGGTGATTTGGCTCcCATATAACTGTGTAGGAAAGGTGGTTGACAGGTTGAGTAGTTtGTATCTTTCTTCCGGTATTGAGTACCGACTCAACACGGGAAAACTTAAGAcgTCTAGACATAGTAACCATTGACAAACTGAGAGCTC<br>TTTCTTGATTCTATGGGTGGTGGACCGTTGGTgTtGTTGGTTCGAAAAaCCGCACCCCCTTAAATCTTGGAAGgAGTTCAGTGAATCATATtGTTAGCCGTTTTGGAAGAGTGTTTGAAGcGTTGGAAATTTACGAAATTTCGGGTGAATAAAATCGGGACATTTGGAGGGTC<br>TCACGAGAAAACTGAAAAATCCTCTCCAGATTCCTTGAATCTGGACAAGGCAAAAGTTATGGGGCTGGTCCCATGATGTGTGGTAACGTCTCAAAATTATTTCAGCGGTTTCAAAATTAATTCTCACCTAGCAACTCAAGGCTTTCTCCTTGTGTATATTATGGAATCCCGT<br>TTCTATAGCCTGGCCCAGGACCATAAATTCGAATTCCATAAGACATATTTTGTTTTTCGTTCATTATGTATTTATGTGAGAGACACACGCAAGTTTAGTTCGCCACGGCTGTTTTCATTGGTGCACTGTGATTTTAGCCGTTTCATTCTGGGATGTAGACTTGTTGGGTATGC<br>AGACAACTTTCAACAATAGCCCGGGGTGAAGGAGGCAACGCCACCACGGCAGTTGGCACAAAAGTTTGTGCCGCAATCCATGTGCAACCCTAAGAGTAcACGTATTGCGGCGAAAACGGTATTTTGTAATTGGGCTAGAGCAATTTCGGCCACCTATTGCAATTGCTTTGT<br>AATTAGGATTCGAGTGTTTTCAGATTAAACACGAGATGAgAaGAGAACTTTTTtGAGAGAGAAGAGAGGACTTGTAGAGTTATACTCCATATTAGAACCCcAAAACGGGTAAGGGTTTGGGTAAAGAGTAAAGATTGATTTTCGATAATTTtACCCAAATTTtGAGTTTGGAT<br>GTTAAAATGGGTCAAAATTGGAGATGCTTTTAGAGATACTGGATTTTGATTTTTAAATTTATCACTTGTTGTTTCTCCACAAGGAATTGATTTTGCACTCAATTTTTTTttATAGATGCaCTCCAAAACTTGTCTTTTATTATTtCAAaTTACATGTAACAAATTTtCAAAGCACA<br>CTTAAAacAaGTGTACTAGTTCTTTTGTTcGTTTAAAaGGgAAAAAAAAAaTaCTACAGTAATATTCAATGGAATaTTAGTCAATTCGGTTTCCCACCGGTGCAAACCAGTATTTGAGCCGGAACCTACTGCTAAACACGCTTGAGAGATCAACGACGTCGGGTGGGTTAGGG<br>AAAAAAAAAaCCTCCAAtACGTCGCAGAAGGGACCTTGTTTGAATTACGGCGGGACTTGCAACTCTCTCTCTCTCTCATGAGCTTTCTCTGTCGTCTATCGTTTTCCTCTATATATACATAGATGTGCCCTCTTGTTCGGAAAGCATACAACCTGTTCGAAGAAATAgCCCAA<br>AGGTGCCCTTTTGTGCGAAACTCTGTACGTTGTTTATCAAACTGTGCGTTGAGTCAAACCCATTCCTATGAAGACCCAATTCAATTTTCTTATGAAACTCAAAATGTAATATCTTGGACCTCAAAAATCTCCGCTTTAGTGAGGAGAGAGAAGCCCCGAGAGGCCATTGGGT<br>TGTTCAAAACCATGCTTGTGAAAGAGCAAAGGCCAAATTATGTGACAATTCTGAGCATCATACGCGCTGTTGGCAGTTTGGGTTCTGAAAATATGACCCAGGCAATTCATGGGTTGTTGTTCAAAATGGGGTTTGAATCAGAAATTCCAGTCGTAACCGCCCTTTTGGGTG<br>TTTACTCTATGTGGGATGTTGAAGTTGTATGGAAGCTGTTTGATCAAATACCCAATAGGGATATGGTTTtATGGAATGCAATGGTTTCGGTGTGCGCgAaGAGCGGGCAATACAGGGAGGCGTTTGAGATTTTTAGAGAAATGCAGTACGATGGTGTTAAACCAAAAGATGC<br>CAGCATTGTAAGCATACTGCCTGCTTGTGCTGCTCTTGCTAATTTGTCCTTGGGCAGACAAATACATGGTTTCTCTGTAAGAAGTTTATGTTGTTCACTTACAAATGTTCAAAACTCACTTGTAGATATGTATGCGAAATGTGGAAATCTTGAGACCTCTATTCAAATATTCA<br>ATAGAATTGAAAAGAAGGATCTTGTTTCTTGGAGGACCATGCTTCATGGGTGTATCGCCAATGATTGCCCTCGAACGGCACTGAATATGGTCTCTAGAATGCAATCTAATTGCTTTAGACCAGATGAACTTTTGATCCTTGATGCAATTGGAGTCTCTACACAAGAAGAAAA<br>ACTTAGTTTTGGGCTGGGATTTCATGGTTATATCGTAAAAaGTGGTTTCTTGGATTTTGTCTCGGTTGTGACTGCACTTCTCCAAaTGTATGCTAGGTTTGGTGAATTTGGTTCAGCTGGGGTTTTATTTAATCATATCACTCAGAAAGATGTTATTGCGTGGAGTTCCATGgT<br>ATCGGCATATGCGCAGGGTGGACATCCTTtGAATGCTTTAGAAACTTTCAAAAAGATGCAATCAACAGATGAGAAGCCGAATGAGATCACTCTAGTCAGTGTATTACAAGCTTGCTCTtCTTTGGGAGTCCAAGAACTTGCGGAGAGTATCCATGCGCATGTGACTAAAGCT<br>GGCTACTCTTCCAACACAATATTACTACCAGCATTAATTGATATATATTGCAAATTCGGAAGGATAAGGCAAGGAAAGGCACTTTTTGATGAAATTCTTATCAAAGATCTTGTCTGTTGGAGCTCAATGATCAATGGTTATGCGATAAATGGGTGCGGAATTGAGGCTCTGG<br>AGACCTTTTCAGAAATGTTGAAGTTTGGAATAAAGCCCAATGAAGTTGTCTTCATTTCTCTTCTATCTGCTTGCAGTCATTGTGGGCTAGAAAATGAAGGCTGGACATGGTTTTATGCAATGGAGAAGGTATATGGTATTACTCCAAAACTTGCACActATGCTTGCATGGTG<br>GATCTGCTTAGTCGTCAAGGACATgTAGAAGAAGCCCTTGAATTTGTGAAAAGTATGCCTATTGAGCCTGATAAAaGAATTTGGGGTGCTTTACTTGCTGGTTGTAGATCAATTCATGGCtCTATTGAGATAGCAGAATTTGCGGTGGAACGGCTCATTGATTTGGACCCGG<br>AAAaTACTAGCTGTTACGTGGTTTTGTCAAATTTGTATGCAGAGCAGTGTAGATGGGAAGACGTGGAGAGACTGCGGGAACTGGTAGACAATAAGGGATTGAGGAAGGAAATAGGATATAGCATTGTTGAAGCCAATTCATAGCATGCATATTGTGAGATTGGTTAGCTGT<br>GTTCTCTCAACAGGTTTCTTCTTGTTTTTtGTTtGACTATCTGTAGAATGCTGAAAATAAGTTGCTGGATATATCTCTTACTCAGAGATCATACATCCATTTTCAATCTCACCTTTTGGTTTGTCCGTACATCACACTACATACCTTAGACAGAACTTCTTTGAATTTTCCAGTA<br>ACGTGAATTTAAATAGGCGTCTGTGAATTTAAATAGCcaTCTATGTATTTATTGTTGCGGAGTTACATTTAGTAGCGGCTTAAGTAAAGAATTTATTGCTCATTGAAAGCAGTACTGTATGCTTCCTTTTATATCCTCCATTAATGCGTATTTCACTAAGTTAACTTTCCAGAC<br>CCTTGTTACTAGTCCAATAAATTCTCGAGATCTTTTCCATATAAGCTTTGATTTATATCCCGTTCTGATGAAAGAAGGAATCCTTGAAGTACTTCTTGTCAGCGCTAAAGACATTAGAAACACCAATGTTATCGGTACTCTGTCTCTCTCgCAGCTAATGTCTAAagAGGCCA<br>TGTGTATATAATATTTACACGGATTATAGAAAGCACTTTGTTTCTTTGTGTGGCATTTTTTGCTTTCTCTTTTGCATTAATTGGAGCTTGTTAATTGTTCGAACTCAACCTCGTGTTTAACAGGGAGACCAGCATATTATGTGCTCATTGAATGTGGAACTCAAGTGGAAAGA<br>ACCAAAACTACAACAGGTCAAATTCTTGGTGATAGTTCATCCATCTTCCTAGCTCTCATCCTAATATGCTTTCCCTAACCTAATTACAGAGTACATTGGTGCAGGTAATCACAAAGAAATATGCTGGAATGAAAAGTTTGCGTTTGAATTTCCCTCTTTCGAATGGGAAAAC<br>TTGACTCACCTCAAATTTAAAGTCATGGCTGAGGAATATTTGTTTGAAGGAGGATTTGTTGGTGAaaCCATGTAAGCAGTTTTAACTCAAAACATTATTCTTGTTCAGTTTTCTTTGTTAATTACCAGCCATATAATGCTTTCATTTTCTTTGTTAAACAAACAATTTTGTACT<br>TTCTGCTAGGaTAAATaTCaCAAATCTTCTAGAGCTTCTTTTAtGTcAAACCCaGCAAGGAACTTGCTCTCTCAAACCATCTTCTACCCTAGTAGTATTGAATATTGATCACACGAAGTTAAGAAAAAaGGGTTTAATTTCTGCATtAATTTTTtGTTTTTtGAAATCTTGTGTAT<br>GCATTAAGACATTTACTTTGGCATTGGAAACCTTAtGAGTTATGAAGGAATGAATATGTCTTAGAGGAACCGTTGTCTCTCCCTCAATTTCTGTATTTCTATAGGTTAAACTCCTCGTGATGATTATGAACAATGTGATTTGAAGCTTTGAGAGCTTTTAACTAGTAAAGCAG<br>TTCATGAAATAATGGAAGTACTAGCTATAATATCTCcTCATTTGCGTATACATACATTTTGTTTTtGTTGAAATAATTTCTGTATCCCAGATTCtcGGGgCAAATTAACTATCTAGATATTG<br>
 +
 +
Got:<br>
 +
 +
[[File:00278.png]]
 +
 +
 +
 +
11B) MYCA1 scaffold 00554<br>
 +
Submitted from SSR primer area<br>
 +
 +
GAAAACTACtAaGAAACATTGTCACAAATGCAGAGGAACACAAACTcTTTCCAAACCAAATGAAGAAACTGAGTTACGTACGCCCAATGTAAACATTATGACTTTTTACtaCTAACCCTAGTGCAAATTATTGCACTATTGTTGCATTATGAAATTCTAAACTATCACAAGAATAATGAAGGGGTTTtATAGGCTA<br>ACCcTAacAACCTTAGCAAAATCCAAATAACCCAAAAGGACTAAAATACCCTGAACGTAAAAcaaaaaTAACGATAACCTTACAGAAACCcTAATGACACAAAAAGACTAAACTACCAtGAACATAAACAATAATCTGAAACCCTAAAttACACTCCCCCCCCcTCCTCcTtCAAACGCAGGATGCGAAGCCG<br>GAGAGCATTGTGAGTTTGCCAAGGGATTAACAACAGCGAATCAACTGCTGTGATGGTGCTGAACAATGCAAAGCaGTACAAAACTAGAATtTGCGAATAGGAAGAAGAACTGATGCCGAATAGATGCAAATAGGGGAAACCAATGCCAAACAGATACCAACCAGATGTCAAATTGCtGATGCAAACAAT<br>ACCAAGAGAGAGAAGAGAACAGAAgCAAAGTGTCAGATCAATTAAGATGCAAAGATCGACAGAGgTGTAGGGAGGAAGAACAGATGCAgAGAGATGCAAAAACTGATGCAGATTTAAGAGGTTCAAAAGTTTAGAGGTTCAAAGGTCCAACATAGCAGAATATTGCAGAATCAGAAGCCCAAGTAAGA<br>GCGCAAAATACCATAGTGGTTGTAGAAGcGGAGGTCGATATAATGTGCACCAGTCAATCGAAAGAAACCCACTCAATTGATGCAATCTGTCGATTCCAAGTGCATTAGGGAgaGACCCAGTCAACGTCGTGGACCAACCCAATCAGTTTAGAAGTCAATCAGAAgTtACCCAGTAAATTTAGGTAGAACT<br>ACCCAAAAGATTATGAACATGATCTGAAGTCATCGTGGCCaGaCTGGAGATTGGTTCGCCCAAGGTCGTCGGAGCAAGTCAcTGGGTGGgAGGTGGCTGCTGCTGCTATTGTGTTGTTGTAcGTGATGTTGCTGCTGGTGCGATGAACAGTTCATgTGAATGCCCCGcGAATGGTGGCAAGCCTTAGGcT<br>gCCcGCCGGCGAGtGAATATCCGACAtgCCTAGATCTTATTGCTCTGATACCATGTGACTTTGCAAAGAaGGAAAACAAAACTAACGTATTATtGCaTTATGAAAGTCTAAACTATTACAAGAATAATGAAGGGTTTtATAGTCTAACCcTAAAAaCCTTAGCAAAaCccTAATagCCCAAAaGAAAaTACCTG<br>GACATAAAAGATAAATAAcGATAATCTTActGAAACCCTAATGACACAAAAAGACTAAACTAcAATGAAAATAAACAATAAACAAAAAGACTAAATCAAAGGTTGGTAACTTTGCATGTAAGGGACAACCCACAACTCTACTAAAAAaGGGGGACAAACCATAGATTTCTTTTCAATAACAGCTTtCTTGG<br>AGAGAGAGAGAGAGAaGCATACcTGTAATAAtCCTTTTAGGTCCAAGCTCCCTCACAAAATCAAGTAGTCTAGACAATGCATCAGGAGTACGAGCATAATCCAcAATCACCCCAAATGCTTGTCCCTCATCTATCAACTCACACCTACCAGGAACTGCATCAACCTCTtCAATCCcTCTAACAATATCCTC<br>CAAAGGTGCTCCAACCGCAATTCCAACTGCAaCAGCTGCAAGAATGTTGTATACATTATGTCTGCCAAGCAACCCCGAAGAAATCTCCAATAAACCGTCGGGCGTTTTTACCAAGACCTGGGTCTCAAATAGAGATAGTTCAAATTTCAAAGAATGAACGTCAGCatCTTTATTCTCCACTGCATACGTCA<br>CAACAGGCACATCTGGGTTgCCTTGTGCAATAAAGGAaGCTGCATTCGGATCATCAAaGTTGACGATTTTCCTGTGTCTTTctGGGTCCACCATTCTTGAAAACaGCTTGACCTTAGCATCCCTATaCTCTTCCTCagaCCcTTCAAAaTCGTAACGgtCCCtGTCAAATTTGTGAAAACGgCAATGTCAAAGT<br>CAACCTCATTGCACCTCCCTAATGCTAGTCCATGTGACGAAGCCTCCATCACAACTGCTTCTGTCCCATTATGCACCATTTTCGCCagCAACTTCTGAACCAAAACAGCATCTGGAGtcGTACTTGGCGACTGCAACTTaTTATCTCCATGAATATAATACGCCACGGAGCTGAACATCCCAGTTCTCAAT<br>CCCATGGCTTCATACAACCCTTTAATCAAATACGCGGTGGTAGTCTTCCCATTTGTACCAGTAATCCCTATCACGGACATTTTCGTAgAGGGGTAaCCAAAAAAAGCTGCCGCCAATGCTGGgAGCACCGAACTTGTGTCTTCCACAATAACCACAGCCTTACAACCTAATGTCTCCTCTAAATCTATCTC<br>TTTACTAGTTACCACAGCAACTGCACCTCTCTTGTATGCCTCCGTCAGATACAAATGCCCATCAGTTTCCCTtCcGACACAACACACAAaCAAATCACCAGCCTCAACAAACCTCGAATCATGTTGAATCCCACTAATCTCTACCTCTAAATTACCATAAACTGAAACAGGTACTACTTTACTCTCATCTAA<br>AAGCTCAGCTAATGTCATATAAAACGTGGGGTCAACAATCCTAAACTTGTGACTTTTATCAATCTTATCGAAATCAATATCGAACGATGAATCAGAACTCAAATCAGACTTaCCCAAATCAGAACTCACCTCATTCTCCAATTTCTCACCCTCTTCATCATCACCCGCATCTGCCGGAATCACAGTGAGCC<br>CTCGCAGCTCCTCAATTTCCTCCAAATCAACCACTTCTTCAGGCTCCAGCTCATCAATACCCTCATCCACTTCCTCAACCTTCTTCTCTTTCCTAGGGTTAGGTTTCAACAAaCCCTTTTCAACAAACTCTTTCCTTGACAAAGCAATAGCCTTATCAATATCAGCAAACACATCATCCCCCGAATCTGTTG<br>GAAAAATATTTTAGAATTTCTGTAAACcAAAAAAaCCAGGGACAACAaTACAActATAGCTACCATAAACATTTGTGGCTACGGGAGTTACATTTGAAATTTGTAACTCTTTTACAACTTTCAATTAGCAAATCATTACAATTAAGTACAATTACAAAGCTATCACTGTTGTAAAATCAAATAATAGGTACC<br>ATGAAAATAAGAGAGAGTTCACTCTTGCCTTGGGcgAGGATAACTTTatCCACATTCGGCGGGgTTTTGTGGGTTCCGATTCGGTAATCAGAACCATTcATTTCGTATGACTTGATTTATCCACATTCGgCGGGGTTTTgTGGGTTCcGATTCgTTAATCAGAACCATTTATTTCGTATTACTTGATTTATTA<br>GGAGACcaTGTGAAAaTTCAGCTTAATCATAtAGTTGTAAGCACTCAaTCCAAAATTttAaTTTTtCTCCATCCagTTCTAAAGCTAaGGAATTaGACTTtCTATGCTCAaTATctAATTGATCTAAATTTTTTTGCGTTGTCTCCTAACAAATCAATTCATCCAAAaTAAGCAGTTCCAATTCCCATGGGTAAAAT<br>TACACTAGCTCTGTTGAACAGAATTGCTCGCTACAAACAATCAAATAAAATTCAAATCACAAAAAATCTAACAGAATCACCACCTCGGTCTTCGAACCCGGGATCGTCGGGTGCGTCCTCCTCGTCGGCAATTGCATTGAGAAAAATTGATTGCTCCTTTTCAGACCGCTCTTCTTGGATCTTTCTTGCT<br>TTATCAGCATGGCGCTTGACTTGCTGGAACCTGGATACCCCGTGCATGGAGTCTTCTTCCGCTTCGGGCGGGTCATCGTCGGATGGGTCCGGTTGGAATACGCCGTcGGGgCCGATTGCGGCAGGGGGTCGGAGTTGGAGTTGGGGTTTtAGGgTTtGGTGAGgAaGAGaGAGGAaaGGgAaGGgTTtGAa<br>TtGGGgAAAAaaTGTtGGATTTTtCAGAGAGGGGGTGAAGAGTGGgAAGGAGATgAAGGTGGGTGGCAATGGCATTGTAGAGAGAGATTAGAGAGAGAGAGAGAgNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN<br>NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN<br>NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAACTCAATTCTTGAGGCCGCA<br>AACaCAATTCATGAGGCCTCGAACTCAGACTTTGTGGCCGAAaTGCTTTGGCTAAAAAAaaaCATTTTtttCTTtGGTCTTCATTAAAATAAAAaTTTGCATtcGTTAGTTTTGCGACGATTTTTGTGGTCTATTGGTTCGTtCcGACGAGATGAATCTAAAgAGtaTAAAAttAAAATTTTtaaagaTTTCACTAAAG<br>ACAAAAaTAATCTAGATTTTTgTgATTtATTAGATTgTTTTtGTCTTTATTCaCAAAAATTTAAGTTtCGTTCATAATTTTAtACTTTTTtAGATTCCTCTCGtGgAGAGAATCCAATAAATCACAAAAAAaTCGATGCAAAACTAGCAAAAACAAATTtCTTTTGAaTTAAAAAAAAaCATTTtAGTTTTTTtAGCC<br>AAAGCAAGTGAACGAAACTTGAATCTTGTGACCTGAAACTcccACCTTGTGACCACTAACTCGAACCTTTTGGCCTCAAACTCCAACCCTTtGGGCCGACAAAATGTtATTtCTCATCTTAAAAActtAGGCCTaTTTTGGCCAAAGTAGAAAATCAAGCTTTtCcTTTtAgCTTTTTTTTTTttGGgAAAAAAaCT<br>CAAAGGTAAAaGGTGGATtGTTTtGGCcTTTTTTttGTGCTTTTTGCTTTTCACCTTTCAAATTGTAATtGTTTTTTtATtACTTTTtGCTTATtGTAAaGTTACTTCTTTAAGCCAAATTCTATGACTTTtCgAaTCtCAAAAAAGCAAAAGGCTTGCTTTTCTAGTTTGGCCAAAATAAGCcTTtATCTCCATAAtttg<br>ttGACTACTTTGTtATTCATGATTTTTAaTCTTTAGtgATTTCTGTCAAATATTTtATTTTTTCCCTTTTTAGATGCATTCATACCTCTgTTTtAAAAAATATAAAAAaaaTTATGATATTAAGAAAAAaGACTAAAAaCgAAAAaTAGGCAACTATATACAAGCCTTTTtAAtCtAaaTAAAAAaCTCATCCAAaTAA<br>ATGCTACTCTAACTTGTTTTtCTCAACACTTTTGTCAACAACGTTTAAaTAATCTTGTGAAATGGAAATGAAAACTATTTTAACGCTCCTTTTTTTCTTTCTATCTATTGgCACTCAATTTCTTATCTTTTATtgCGTGACAAAaCTAAACTATCCTTGgTtCTTATCTCTTGAGTCAATTTTTtCATATAAAAGG<br>TAaTTCGAtACTtCCGCATGAATAAAAACCAAAAAaTGGGTGCCAATGGATAAAAAaTTaGCGTTAAAatCATTTCCCAAAATAAAaaTaGTTgCGTCAAGGTTCTAAAtaCCATAtCGTTTTTTCtAGTTGTACAATATGAACCAGtNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN<br>NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGCACTATGCTGCACCCTCCTCagATATTTTTGGTCagCTTCCCGCCAAACCCACTAGgCTAAAACCCTAGTTGCCTTTTCCCC<br>CACTCCAaAACCCTAGTTCtCTCTCTCTCCCTCCGCCACCAGTTCCCCACCCcACCACAACCAACCATGGACCCTCAGTCcGTTCAGACAAACCTAGTCCTCATCCTAGACTTCGGTtCTCAGTACACCCACCTCATCACgCGACGCATCCGATCCCTCTCCGTCTTCTCCCTCTGCATCTCAGGCACCTC<br>CCCTCTCAAATCCATCACAGACCtAAaCCCAAGTGTCATAATCCTCTCCGGTGGTCCCCACTCGGTtCATgCGCCCAACGCGCCGTGTTTCCCtGATGGGTTCATAGAGTATGTGGAGTCTAAGGGCGTGTTTGTTTtGGGCATATGTTATGGGTTGCAGCTGATTGTGGAGAGGCTGGGTGGGTGTGTtGG<br>GgTtGGCGAGAAGCAGGAGTATGGGAGGATGGAGATTGAGGTGGAGAAGGCTTGTGGTTTGTTTGGTGGGTCAAAGAAAGTTGGTGATAAGCAGGTTGTTTGGATGAGTCACGGGGATGAGGCTGTGAAGTTGCCTGATGGGTTTGAAGTGGTGGCGCGGAGTCAGCAGGGGtcGGTGGCGGCTGTGG<br>AGTGTCCTGGGAGAAGGTTTTATGGCCTTCAGTATCATCCGGAGGTAATTGGGTATCTTCATTCATTTCATTCTTGGCCTTGTGATACGCTGCCCTGTTTTAATTGTCTTTGTAATTTGGTtCTTTGGCTGGCTTTGTTTGGTTCTTTACTAGTGGTTATTGTTTGTACGCGAAGTGCTTACTTGCTGATATC<br>ATCTTCATGTTGAAGTGGTAAtAATGAATGAAAAATGGAATGAATTGACCCAATATCTGAAAAGTTATCGTCTTTAGTGGTACTGTGGTAGTGGCCTATAAAACTGTTAGCGAcACTCCCTCGtGATGTATCCTGCTCAGGCCcAGATCCTTAGCAAGAATGAGTCAATGACCATTGATCCCTTTtCAAGCA<br>CTTAAGCtgAaCTTCCACATGgTACCATAAAATCCCTGAATCCTCTCAGCACAAGCACTGATGATCTCTCTGTTTGAGTTTCACATCACTAGCCCAACCCCCAGAAAACcATCCTTATAGTTATAGTAGCCCCGTCAAACTGAATTGTGAGCTCACCTTCATTTGGAGCTTTCCATGAGCCAGCAAGATGTT<br>GGGATAGTACTACAGTCTTTGGAAATACAATGGGAGTTCTCTCAGGTAGCTAGCTGCATATTCtGCAAGATCACTGGCTCCCCTGTTTGTCCCtccAAAAAaTGTAATTGCCTATTTAACCAAATTACCCAAGCCACAATTAGAAagAGATCTATTATTtGTTGGAACCAACCCCCTCAATCTGCATAGCCCA<br>GAGATGCCCCTTTCTTCAAGCACATGCAAATCTTTGAATCGATAACCTTCCGTAAcATTCTTTGCTTACTCACaCtCTTTTAGACAACGAATGATGGTCTCCGGTGCGGCGCTGTCCCTCCACACAGGCTACAACAATCCTTAATATTAACCATTTTTTtGACAAGCTTATGGTTCATTGGGAAAATCCCCA<br>AACACAtCTTTCAcacaatgaCACAAACAATTTACTTTgTGTTGGgAaTTTTCAGATTCCAGATTTCTCCTAAGATCTcATATTATCCATGTCAGAAGATGAGAGTTGAGAGTgCTGAAAATGTAATGTCCCTTGTGGTGTAACCAGACtCcATGGTGTAAGTTTCTTGTTTAGTGAAGTTCCATGTTTAGTTGT<br>CTTCATTTCCCAGGaTAATAGGGGAATGTTTCATGtCcTATgAGCTTCAAAAGGGaTAAAaGATCACAGCTAAGATGTTCCTTCCAAACCCCAGTATTGTGGTCAACGAGGCCCTTtACTaCCATTCTCTCCGTATCAGGCTTACAaGAGCTTGTAGTTTTAaGGGAGAAGTGCTTGGgATCCATtGGTCTGG<br>CCACACCCTGGTTTtAtATCCcTTTCcGATCCtCcACCTAAGaCCCTTTTTAAGCACCTCCATACCCTCACACAT<br>
 +
 +
[[File:00554.png]]
 +
 +
 +
 +
12) UFGT <br>
 +
search with amino acids using tBLASTn<br>
 +
SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL<br>
 +
 +
[[File:UFGT_EST.png]]

Latest revision as of 12:42, 17 April 2012

This is where I will capture my progress on this project.

I will also be working on the 13 genes Allan gave us.


SSR Primer Hunt

The class has already completed 4 SSR primer designs (LDOX, CHS, StSy, and F3H).

Results from Blueberry 454 Scaffold Searches from Grape (given to us by Allan Brown)
AOMT Scaffold 00796

  1. Forward Primer GAGCTCTTGGATGCTCCATACT & Rev Primer CCTCATAATTTTTGGTCTTCGC (taa) x6 PCR product = 137 bp
  2. Forward Primer TATCCCATGTTTTGGCTAATCC & Rev Primer TTTGCTTTTCAATCGGGAAC (tc) x9 PCR product = 261 bp


AOMT scaffold 01781

  1. Forward Primer GGCGACTGTGTCTTTCAGTAAA & Rev Primer TAGGATTTCGAGGAGGAGAGGT (ct) x7 PCR product = 136 bp
  2. Forward Primer ATAAACAGGTAAACCGAACCGA & Rev Primer CCGATTAAATTGGAGCAGTAGC (tg) x7 PCR product = 262 bp


ANPER scaffold 00666

  1. Forward Primer TTTCTATCTCTCTCGCCGTCAT & Rev Primer GTTTTCTCACCTTGTTTTTGCC (ct) x21 PCR product = 219 bp
  2. Forward Primer CCGTTTCGATCTCTCTCTCAGT & Rev Primer CATCCCTAGATCTGACACCACA (tc) x14 PCR product = 278 bp


CHI scaffold 03186

  1. Forward Primer TACATCTTGAGGGGCAGTTTTT & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
  2. Forward Primer ACGTGGTCCAATCTAACCTCAC & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp


DFR scaffold 03132

  1. Forward Primer TACATCTTGAGGGGCAGTTTTT & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
  2. Forward Primer ACGTGGTCCAATCTAACCTCAC & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp


F3'H scaffold 01438

  1. Forward Primer CAGTGGTACAGTGGTGGATGAC & Rev Primer ATTCCCTTTCTCTTCCCATTTC (ag) x7 PCR product = 152 bp
  2. Forward Primer ATACCAATCCATAGTCTCCCGA & Rev Primer TCAGTTGTGTTTTTCAGTTGGG (ta) x7 PCR product = 279 bp


FLS scaffold 00142

  1. Forward Primer CAATTATACCCTTCCTGTCCCA & Rev Primer GAATGGATGAAAGGAACCCATA (ag) x8 PCR product = 214 bp
  2. Forward Primer CTAGCTCTCCCTCTCTCCCTCT & Rev Primer GTGGTACTCCATATCCATGCCT (ct) x8 PCR product = 295 bp


FLS scaffold 00374

  1. Forward Primer TGAGCATCATTGCCAGAGTAGT & Rev Primer GGATTTCATTCCAAGAGATTGC (ct) x6 PCR product = 295 bp
  2. Forward Primer TAGATTATCCGGGGAATCATTG & Rev Primer GATTCTCATGACAAGCCAGTTG (tc) x6 PCR product = 275 bp


MYBA1 scaffold 03500

  1. Forward Primer GCGAGGATGGAAATATATACGG & Rev Primer GCTTCATAAGCTGCTAGGCAAT (ag) x8 PCR product = 222 bp
  2. Forward Primer GCGAGGATGGAAATATATACGG & Rev Primer GCTTCATAAGCTGCTAGGCAAT (ttat) x5 PCR product = 222 bp


MYCA1 scaffold 00278

  1. Forward Primer CAGTATTTGAGCCGGAACCTAC & Rev Primer CGTACAGAGTTTCGCACAAAAG (ct) x7 PCR product = 268 bp
  2. Forward Primer TAGTGGGAACGAGTCGTCTTTT & Rev Primer AGTTGGTGCCCTTTTATGAAGA (at) x6 PCR product = 195 bp


MYCA1 scaffold 00554

  1. Forward Primer GGCTAAAACCCTAGTTGCCTTT & Rev Primer GGGTGTACTGAGAACCGAAGTC (tc) x6 PCR product = 157 bp
  2. Forward Primer CCCAAAAGAAAATACCTGGACA & Rev Primer GGAGCTTGGACCTAAAAGGATT (ga) x8 PCR product = 260 bp


UFGT scaffold 00304

  1. Forward Primer TGAACTACCAAAATGGGACAGA & Rev Primer NGACGGAGCACATCCTACTACA (ag) x10 PCR product = 207 bp
  2. Forward Primer CCCCAAATTCAGATCAAAGAAG & Rev Primer CTTATCTCTGCCCACCTACACC (ag) x12 PCR product = 230 bp


UFGT scaffold 01120

  1. Forward Primer TGTGTGTGTGTAGTGTGGTCGT & Rev Primer AAGAGAGAGGGGTCCTGAAAAC (ct) x11 PCR product = 132 bp
  2. Forward Primer TAAGCTACAGTACCTCGCCACA & Rev Primer AAGGATATGCCGACGAGTCTTA (tc) x6 PCR product = 196 bp


EST Search Results

For the second half of the semester, we focused on finding ESTs of our genes using the Washington State University Vaccinium genome database as well as the Towson State University Blue Berry Genome database using 454 sequencing.

Download my PPT summary.Media:Allans_ESTs.pptx


ANPER - anthocyanin permease

AOMT - anthocyanin O-methyltransferase

CHI - Chalcone isomerase

DFR - dihydroflavonol 4-reductase

F3’H flavonoid 3' hydroxylase

LDOX - leucoanthocyanidin dioxygenase

Myba1 - myb-related transcription factor

MycA1 - myc anthocyanin regulatory protein

UFGT - UDP-glucose:flavonoid 3-O-glucosyltransferase


1) ANPER Submitted the 3’ most blueberry sequence from my scaffold00666
ggatggcaagctctggtagcttacatcaacttgggatcctattacat
Got this result from BLASTn search of Vaccinium database
>DR067294
Length = 260 Score = 60.0 bits (30), Expect = 9e-11 Identities = 39/42 (92%) Strand = Plus / Plus 

DR067294.png


2) AOMT 01781 search of Vaccinium database
Submitted carboxyl most amino acids that gave hits with my scaffold01781
Did tBLASTn
EEMVLEDLRPSRESTMKLNKMLAADPRVMISHASIGDGITLCMRV
No hits.

I submitted
ATCGAGCTAATATTTTATGAGACcACTTAAAAAaTTTAAAAAaTTATTTtAATATTAATGAACGATTCGGCTCATTTGTACTCTACGGA
From the scaffold

And got
>DW043195
Length = 523 Score = 48.1 bits (24), Expect = 7e-07 Identities = 33/36 (91%) Strand = Plus / Minus 

DW043195.png


3) AMOT 00796 search submitted ATCTAAAAATAAATAAATAATTTGAGTAATAATTATTAAAGACGAAAAAaTAAaGATGACGAAATAGTAAAAGCTTTGGAAGATAAATTTTCAAACCTGGAAACCCGCAAATTACTCCCACTTGCATATATAAGTACTCA
TATAATTTTCTATTATTTAATTTAATGGGTCCTAACATGATTGTAGTTATTATTTTTTTTtATTATTATTGTAGTTATTTATACTACGCAAGATGAAGGGTGCACAAGTCTTCCATGTTGTGGAATATCGCAGCTACAAGCA
TTTATTTAATGGGTACAAAATATTTTTGTAAGCGTCGtGCCCATTATAGGTGAGCATATAAGTCTATAAAGTATTAATTTAGAGAGGTCAATTAAAAAaGAaTCTCCCTGTGACACTACAATCGTGCTTGATGTCAACAAa
TATGaCAAAAAAaTATATaTAAAaTaTAAAacAAgTACTTtCAtgcATtATTtaTTtAAtATTTttCAcACAAATTTGAAATTGATGCCGAAAAATTAGAAATTTCACATAAAATATTTATTTAAaTTAAAAATAGCACGGTTTTTC
ATAAGGAACTAAAATTACTTGGCGGACGGAATACCAGAAAATCACGCAATTAATTCTTCAaGTTTTTCACTTTTCACGGAGGTGGGTTTATGATCtCGTAAATAAGTAGTTTCTTCTTTTTTtATTGTGGATATAATCTCCT
TCTATTTTCTCTAAATATTGAGGATCATGATAAtGCAAAGATGCAAGTGGTGGCTAGCTCATGCGGTAAGAGCTCTTGGATGCTCCATACTGCATCCCGAGATCGAGTCCCATCTCCCTTCCTAATGTAATAAATCAGGA
ATGATGAATGAAAAaTAATACGGAGTAATAATAATAATAATAATGCGAAGACCAAAAATTATGAGGATTTTTTtAGGAGGGTTtCGAATTACACCATCcTTTTTGGTAGGTGAATGGTTCAGTGCATGTGAGCATTTTAAT
AAAATCATTACATATTTTGGtATCAAATGGTATTAGTATATATAATTAATTAATTAAGTAATTATGACCCCTTGCATGTGAATGAAGGTGGTGGTGGGACCACCAACACCAATACCAACTCAACGGCATATACATATTGG
AATCGTCAATAGcGGGCAAGTGTGGTTGGTGGAaGgTTGGTGAAATGAAACTTTaGtCcACGTAACAaGAAAaGAGTTGGTGGCCATCCCcTTCAAGGGATATCATTTTCTTTTtAAAGGGCATaTAAAAAAAAAaTtAAAaT
cACTAACAAGAAATTTTAAGGAAAaTAAaCAaGCCCAACcaTAAAACGGTAgATACCAAaTCTATACCACCTTATAGTATATCTATTTTGgAATTtCACACTAgTGATCAAACGGaTGTGGGgTTgCTGCTATGCAGCCCTAG
CACAACAAGTGCTGTGTATTTGGCTTGTTCGTTACTGGGATGGACggCCcAGATTTTACCtCGGCACCTTTGCATCGAatCGTTTGTtGTCGTGCGTGCAAGTGAAATCTGGATCGTCCATCAGAGCAACGGATGACCCGGA
TGTGCATAGCACCTGCTGTGCACGGGGgTgCACAACAGCAACCCcAGTTCTGATCAAACcAATTCTATTATTTtAAaGATTATTTTATGGgTCTTtGAAaGAAAAAAAaaCTCATTCGGATGTCGTTAAGGGCTTAATAGGAC
GAAAATCGATCAAGCATTTAAtGATATtTGAATGAGTTGATTTTTTataGagACTCTtAAGAAtaTGTTTTAAATCAATTGAAtGACTCTgaTCATTTTTGTGGAATTCCAAAATAATTTTtACACGATAGGGTAAATATTTGGCa
TACACCACATGGTATAaGCAtAGTTTTTTTTTTTttGgtGCCTTTACTTGgTTCATTTCCTTTATTTTTGCGAGTCTAAGTCATTATCGTTTCTAGGGATGGCAACGGGTAAgaCATGGATCAGGGATCCTATTTTACCTTCGA
TCCTTAAATGGGAATTTCCTTTCCACCCCcACcTCCATATCTAAAAGGGaTAGTgTTttCCAcgATACCCGCCTCCCAATGGGGATCGgaTACACTtGGGTAAATtcATATCCAAACTAGTTGAAAAAaaGAACAaTAAGATCT
AAAtAAAAATAaTTtaCgTGTCC

BLASTn
Got 3 EST hits

AMOT 00796.png



4) CHI

I did BLASTn with (from scaffold near SSR primer) TTTCTTGAGGTCTTCCAGGATGAGACATTCCCCCCTGGTGCTTCTATTCTTTTCACCCAGTCACCCCATGGGTTGCTAACGGTCAGTACATCCTTCCACAAGTGTTAGTTCTGCCTTTCTCTTCGCCTTTtGTTCTCTCTTC
CTTGTTGATTATAAAATAGGTAATGCAGTTCCTTGATTCAGGAACAATTTAAGCAGGTATCCAAATTTTCTTTtGAAaTTTGAGGgCAAGGAAATCACGATGATTTATGAGACTGATGTTGCATGTTAATTtCAGCTTGTAC
CCCAGATGCAGCATCGATCAGGACATTGTGAATTTCTTTtGTTTTtGGTCACCATTACTTGGTTCTCTACTAGTTgACGGTTCACTGTGCACTAaGCTCCATTTTGATGGACTTCATAAAACaCGATGCAGGACAagATGTA
AACCAGGCCCATGAAAATAGTTtCGTGTTTTAAATCaTTAAGCCTGTTCTTGACTTCGCCAAGAAGAtCAAACTCGTAGTATAAAGTATGTTGTTGAGAAGtGTTCTGAAAAGTAAGcAAATGGGTTTCTATTTtGGTTTTCA
TCTTTTTATAACACTCTTGGTTAATTTTtACCCTAAAAAaTACTCcATTAaTtAAGACCTAAAGACAAcTGGTTTGGGTtGATTATAGGTTTACTTTTATCTGTTtGGTGACATCACTAGATaGGCTGTGTTGCGACCTTGCCGA
TTGGTTTtCACAATCGGAGCACCTGAGTTATGAAGAGACATGCTTTTTtGAGGAACTTTAGGCCCGATATCGTCTTAACTCTTGAGAATCATTTTCTTCATTGATTTGGACATACACACACATGCATTCGAATCCGTGGTGC
AAATGATTCTGTAAGATACAGGTCATTTGAATCTTCATGCTCAAAACCCCCATTCAATTAGCGACTTACTTCTTACTTCCCAAGGAGCAGGAACTGCTCTTTTAACCCTTCTCCGAGGATTACATCCACAAACACAAAGTC
GATGGCAGACTGATATTACTCATCATCATTATCTATTGCATTTATTTTTCTTGTTGCTGATCATATGTATTTGACAAACTTTGGGATTACATGAGTATGTACATCCACAGATATGCATTGGTGTTGCGCGTGTATGTAGGAA
GTTGCAATGAAACAATAACGTTTTGATTTCCTATTGTTGATGCTTTATTTTCTGACTACAGATTGGCTTCTCGAAAGATGGTTTGTTACCTGAGACTGGCAAAGTGGTAATCGAGAACAAACAATTGTCCGAAGCAGTGTT
GGAATCAATCATCGGGAAGCATGGCGTTTCCCcTGCAGCAAAGCAGAGTTTGGCGGCAAGAGTgtCTGCATTGTTCAAGGAGGCATAAGAAAGAAGGTTTTGGAAAACGATAAGAAAGAAGTCCTGTAGCAGAAAagttAg
TAGtAGTTtATTGAgagaGGAAGATAAAATGGTAGAATCTCACTTCCCAGTTGATTCTGATGTTTCTCCATGAGTAAATGTATCTGTACGCTCTTTCATTTCCGTTTtGTCACTGTAATAAGAAGGCTTCAaCTTTTGCATTCT
AAACTCATCACATCTATCTACGACCTTTTCTTATTCTTCTTTgCAAATTTAAACCAAACcGagCCTTATGtCCCAATAaaTTGGGgTCTACTATTGGGgTCTACTATGtAAatCCcTTTTTttGtCcATTGGgATCTGtGCAAATTC
AAATGATATTGAAATTAAaTTCGGCATAaTGGCGGTAGTGGTTATGGACATCATGAATTAGTAATGTCAAGTGTGGAAATTTTtGCTTGTTTtACTTTGtGTAATGCAAGATGTCGAGGCCAGTATACAAGGGTGGAGCCAA
GATTTTAGGTAAgCGGGGtCAAAAATTAAACGCATATGCAATATAtATTTTTTCtATTTGCTTATGCAAAGAAATAACTAATTTGAGAGTTTAACTATAACGTAAATATACAATGATGCATGTATTTCTGCTTTATAATTGGT
CCCAATGACTTATTATATCTTGAAAAaAaTCACTTATTCAGATTTTTtACTTATTTAATAAAAAAaGTGAGATTATATtAGCTTCAATTTGTAGTTAATTTATTTTAAAGTACTTGAAAATGTTAAAAAaGGTTTGACCAAAAa
GTTTATTTATTTTCAAGAGTGCTTCTATAAATTCCTTAAGACAGGTAATTAATTAGAAGTTATTCTACAACTTATTTAACCAAATAATTTACACTCTTCTTTTAACTTATGAAAGAGCACCAAGCGTATCTATAAATTATTT
ATAATTTTTAACTTTATACTTCTAAAAaTCATCATTTAAAATTTtAACTTATGAAGGAGCAAACAGCTGGgATTGATTTAAAATCTTCGGGACTAGTAGAGCCGCAAGGCCCGCAAAGACTTGCTTGgTTTTGCATACAGA
TTGCCCATTTGATTTCCTTTTTATGGGCCTTGTAATTTGGTCTGATTTAATCAATAGAAACTGCGTTTGTTCGTCCTAAAAAAaGGTTagATAAAGATCCTCTTATTTGATGATTTCTATTGTGATGCAAAACGAAATTACA
TAGCAAGCTCATGTATGTATTGGAGAGTTCAACACTTTATAGCTTTTTACGATAACTCGTATGTCTGGGCTAGTTTCAGTTCACCTCGAGtAATTTTGGGGACcAGTCGACTGTTTACTAGTGAGAGCTCCAATTAAAGCT
AAAGAAGCTCCGTATGAATTGACTCAAAaGAGTTGAAAGCACCTAAGATGTTTCGATCCTAAAATCTTAAAAGGAAGCAAACCCCTAAGTTTTAGGGTCATCAGGCCAACTGCTTAGGgTTAACTATTTATAGCTTTTAA
CTTGGGGAGGTTGTAATGGTTATTTACAATGATTAAAaGAAAAaTTATGCATAACGACAAAtCCAATAATTAAAAGCTTCATAATCCCTTCTCTCTCTAAAACGAAATCCTCTCACTCTAGAAaCCAAAAACCCTTATTTCT
GTCTTtCCTTTtCCcTTtCCCCcTAGGgCTTCACCCATGCGGCAACAGTGTAAGAACCcGTGTTTTTAAATGATTTTTCAGAAATAATGTTGGGAAAATGTAAGGAAACgAAAATAGATTAAAGTGAGCCAATGTGGTGTTA
ATAGATTGCATGTGAGTGCATTTAGAACACATTAGAGACATGTGGCGTGTGTGTGCAATTAAGGGTGTGCATATACATCTTGAGGGGCAGTTTTTTTTTtAAAaCtAAAaCACTTCTCTCTCTCTATAGGgCCcGCTGCCC
CcTTTCTCTCTCCcTCTCCATTTCTCTTGCCAAATCCATATCCCACACTCCACCTCTTTAAAATCTAACCTTAAATCCTTCATCTATGCGTGATTTGGAGTCCGAGTGTCTTTGGTTTCACTTGGAGgAGGTATATTTtCGG
TTGAAATTCAAGAACAAAAgTTGGGGTTTCATTTCTCACCAATTGGAAGCTAAATCTTTGAGGTAGTGGACAAATTTCTACTCCCTcTAGTTGTATTTTGTTGATTTGTCGCATATACCATTGTGTTGCCCACAAATTtCTA
GTCAATCGTGAAATCTCCCTAGAAATGGGTTTGGTTTTGATTGAGTTTGATTCCCACTTGTTTGGTTGTTAAATTCACAAATTGGTGGTTGATATACCCTTATTCCATGCTTAAATGATATTTGAAATTCCAAAATTGACA
TGGGTTGGAACCCCCCTTCATTTGTGAATtGCTGTTAAAAATCTCATTTTGTAGcAGTAGATACCAAGTTAGTGAGGTACGATACCTTTTGGTTTTGTTCACTGATGGCGCGTTGGTATCTATACCAAGTTGGTGAGATAT
GGATACCTGTTGGTTCTGTTCATTGACGgTGCACTGGCATCCATACCAATTTGGTGAGGTATAGATACCTTTCTTTCTGGTCGCTAAtGGTGCATTGGTATCGATACCACTCTTGGAGGTATCCAtaCCTTTTTAAAAAaGT
TCCAAAAAACTCTCATTTGGTTTCCGGTTGACTTATTTGGTATCATACTTGATATATTCACCCCGTGCACTTTATTTTTGGTCGAATTGCGCCTTTCTAACCTACTTtCATATGATTGGTGATAaTGTTGATCTTCCAACGTA
CTTTGACACATatAAACTTTGTAGAAATTGGCGATCTACGTTACATGAAATGAAAATGTATGATGTTTGTTATTTGGACATGTGTGAAATACTTGGTGATTTttGAACGAAAAaCAAGTTGATGCACTAGGATCACTACTCAG
TGAGTCACTAGGAAAGGTTTCACGAGGCCCATAAGACTCGGACAAGGGTTCTACGAGGCTCATAAGATTCGGATATGAGTTCCACgAGGCGCATAAGACTCGGACATGGGTTAAGACTCGGACAAGGGTTCCACGAGGC
CCATAAGACTCGGATAGAGGTTCTACGAGGCCCaTAAAACTCGGACATGGGTTCCACGAgCCCATAAGACTCGGACATGGATTCCACtAGGCCCAAAAGACTCGGAAGAGAATTTCAATATTCTCAAGCTTGAATTGATGT
ACACAtGAGATTGATAAAATGTGAAAATGGTTGAaCGTGGACATTTATGGGATACGCGGATACTCAGAGACACAGTCGGGTAGTTTACGGgCATGTTGGGTTTTCTGTTATCATATGTGGGTCCTATATGGACATTTGACCAT
ATTTTTACCTTTGGACATTTGTTGGTCATTCGTCACATGTTTCGAATATTtCGTAGTATCCATTGGTTGTATCACACATGATAGTCATTCTCTTATGCACATATCACACTTTATACACCTAACGTTTGTAGAATGGTTTtCCTAA
TGGGCTAGTGAGCTCACCCTATCTTTTTCAGGTACATGGCAAGAAACATGTGTTGGATGCTTGGAGTGTGGATGCATtGCATGAAGCTTGTTTGATCATGAAGTCATTTTATCCTCCCCATACTTAGTATATTTTTGGGAGAT
GATCATGTAACTTGTGAACACCCCGCcGGAGTTCACGAAAATGGTTTAAGAAAGCAGTTTCGATTgATTGGA

Got
CHI.png


5) DFR

I submitted the cDNA from grape
ATTTTCTTCCCCGGATCATTTTTGAGAAGTATGGGTTCACAAAGTGAAACCGTGT
GCGTCACCGGTGCCTCCGGTTTCATCGGTTCATGGCTGGTCATGAGGCTCCTGGAGCGCGGCTATACTGT
TCGGGCCACCGTTCGCGATCCAACTAACGTGAAAAAAGTGAAGCATTTGCTGGACTTGCCCAAAGCGGAG
ACGCATCTGACTCTCTGGAAGGCAGATCTTGCTGATGAAGGAAGTTTCGATGAAGCTATTAAAGGCTGCA
CCGGCGTCTTCCATGTTGCCACACCCATGGATTTTGAATCGAAGGATCCTGAGAATGAAGTGATAAAGCC
AACTATTGAAGGGATGTTGGGCATAATGAAATCGTGTGCTGCCGCAAAGACTGTCAGAAGGCTTGTATTC
ACATCCTCCGCAGGAACTGTGAACATTCAAGAACACCAACTGCCAGTGTACGATGAAAGCTGCTGGAGTG
ATATGGAATTTTGCCGGGCTAAAAAGATGACTGCATGGATGTACTTTGTCTCCAAGACACTGGCTGAGCA
AGCTGCATGGAAGTATGCCAAGGAAAATAACATTGACTTCATCACTATCATACCGACTCTTGTGGTTGGC
CCCTTCATAATGTCATCAATGCCTCCAAGCCTCATAACTGCTCTTTCCCCGATCACTGGAAACGAAGCTC
ATTATTCAATTATACGGCAGGGCCAATTTGTTCACCTGGATGACCTCTGCAATGCTCATATTTACTTGTT
TGAGAATCCTAAGGCAGAGGGACGTTACATTTGCTCCTCCCACGATTGTATCATTCTCGATCTTGCAAAA
ATGCTTAGAGAAAAATACCCCGAGTATAATATCCCCACAGAGTTCAAAGGTGTTGATGAGAACTTGAAGA
GTGTCTGTTTCTCCTCCAAGAAGCTGACAGATTTGGGGTTTGAGTTTAAATACAGCTTAGAGGACATGTT
TACTGGAGCTGTGGACACATGCCGGGCCAAGGGATTGCTTCCCCCTTCACATGAGAAACCTGTAGATGGC
AAGACCTAGAAGAAGAATTTCTGTCAGGCTTTCTAGCGAGAGCGTAGAAATGTCCTTCATTCTGTGGAAT
CAAGGTTTTCTGGTAGTTTGATTTGGCCAACTATGTATGAAGTTTGTCTACTATTTTATCCATTAAAGGA
AATTATTCTAATGGTCAATGTGCCGGAAATCAGAGTTCGTTC

Got back from BLASTn search:
DFR.png



6) F3’ H submitted from scaffold (near SSR primer)

ATCAGCCAAAGTCACCAGTCACAGACTATCAGCATGCACCCTGGGCCAAGATCAAACTGTCCGTGTGCAATTAAGGTTTGATTATAAAATCCCAACAGTTAAAAACTAAGTAGTCATTACATCACTTtGGCTACTTATATATTCA
CCTAGATAGAATCAACCAACAGGTTcGTATGGCGATTCAGAGACACATGAAATACCATTGATGACAGGATATAAACACACCAACACAAATGCCTATtAATACAAATGAAGAGCAAGCATGAATTTTGTGCACATCATAAGT
TCATTAGCTAGGgCATAAATGGATACAATCTAAACGAGTCCTCAAAGTGCCGGCATTTtGTCTTGTTTTTGTCATTATTGTAAGAGCTGCTTCCTGCTgATAGTGCATTcTCTCCAAACTAAACCTACTAGGTACAAAACCTT
CATGGCTTCTTCAAACCTGGGTGGCTACCAAAGTACTACTCACCATTACACTAACTGTACTGACAACTTTCTTTATCTTGTTAAACCTGCTGAATACTTATCATTTGTAGCTTGTTGCTTATGTTAAAGTTGAGCTTGTTAA
CAAGCGGCAAGGCTTGTACCATGATTGGGTTACTATGAGACTAAGAGCACATACACGTCTACCGGCCTCCATGTGACACAAAGAGCAGAGTGACAATGCAAGGAGGTAGTGCATAAATGAAGCTTACATTGGAACTTTGT
AAAGTTTTAAACTTCAAAAGATGTGTTTTCTAAGAGTTATGTCTAACTTTTAGTTTTGAATGAAAAAACATGACTTTGCGAAAAGATGTTTAGAATCCTATAAATATAGGATCACCCCACTTACTTAGCCTAAATGACTTTT
AATTCTAACAACTCTCCGTATTATTTTCATATATTATGGGcAaTTcGATTCCTTCTTGGCACCCCCGTCTtCCACTGATCGTGTAACCTGGAGACAAACTGCGAGATCTATGGAAGGTATCTTGGAGTCGTATTCAGCCTTTG
GAACCTTCATGAAGGGAGGCAAATATGGCTTTAGGATTTATCCAAAAATATGGCTTTAGGACAGcGATTTGTCGTGCCTTTCATCGATTtCACATGCTAGTTTCCTAATTCTATTCATATTACATATTTGTGTGAttGTGTTCA
TATTTTTAGTGTTCAATGGCATTTCTAATTTCCTAGTGTTTTGTGATAATCATGTGACAAAaTCCCAACAGCTTATTCCATTCTAAGGCAGTAGTGAAGGAAACGACAAGTCAAAAAaTATGGACACAACCCGACACTCTAC
GGACAaCTGTCAAATACACCTAAATATGTGTCTAATTTTTCTGTACTAAATTTTCAAATGAACATGTTGTGGACTCTATAGGAAAGGTtGGGGATTCTCTGGGGCACGTTCAAGACACATAAGGAGTTAAAATGCAACTTTT
AAAAGTGGAGTCAAAACTTCCATTGCTGTTGAGTACATTAATATTAATGTAGTAACCATTAATTGGcATAATGTTAATACTATTGGTGTTTTTTGTGTTAATTtGATAGAAATAATCTTTTAACTTTTATACGAATTCCTACG
AATCATTGACTGATATAAATATACATTATATATtCCACGTGCCcTAACGTGCCCgAaTCTTAGTTTTTACGGAGAAGACATCCGTGTCCATTTTGTGTCCATATCCTTGCTTCTTAACTGTTAGATAATGACGATcAAAAATT
CGATCACTCCAAcGATTGAGACACTGCGTATAACACCAAACTCTCCACCAACTCTCTCACAAGTTGATGTGTCCCACACcTGCGGGGCTTGTGCGAGGGAAATTGAGCTGTTACAGTCTAACTAACGAATGCCAATTCAGT
GGGCTAATATTTAAGTTATTGTGTTCGATTTCTATGTTGTACTTCCATAAaGAAAAATTGGTGCATCTGTTGGAGTTTGTCCCACATTGGTTAATTAAAACCTCCAAATCTAGTATATGAaTCTGGGCAGCCTCTCCACTCA
TTGCCAATTGGTTTTGAGTTGGATGCATTAACATGGTATCAGAGCTAAGCTTTCgCAGGCTTTGGACCAATCTCTTGATTGATTGCCcTCTTgTtaGTGCGATAAAGTGCACCTTGTTTCAATATAATCCAATGTTACGGATC
TTTGTTTACCTCTCCCCATGCGGGAaCTAGACAGGGGTCGCACATGCGGAGAGAGCATCTAGATCTCAAGGCAAGTTGAAGTGAGaaaaaaaaaaagaaCAGTTTGTGATAAGCAGCAGAACTGAGACATCAGATGCTTGCTT
AAAAAACACCTTTCGATGTAATAAACAGATGGGCTGCCAAGCATGTGAccTTTTAAAACAGCaGTTAATAACATAGAAATCGAAGGTCTCCTATCTCACTACAATATCTATCCCCTAGACAATCCcCCTAAGTgTCTCTTtCG
AGAcaGTTTTCACATTATTATTGTTATTGTtCCACATTGCTTGGGTATGGAATGGGAGATCACTTAATAACATATGGAACCTCTCCACTAATTGCCAATTGgTTTTGAGTTGGATATAAAGTTTCCAAATGGTATTAGAGTGG
gCCAATTCCATCCCAAATTTCTTAACAAAAaTTAAATCAAGCCCACACCCCACGATGACAGACCCAGGAAAGGTTGTACgATGATAGAACCCAAAAATTGGCCACAGGTGACAGACCTAAATGTGGCTGCAGGTGAGGGG
GGAGTAAGAATAGTTGTCTCATATTGCTTGGGTATGGAATGGGAGATCACTTAATAACATTTGgAaTCTCTCCACTCATAGCCAATTAGTTTTTGAGTTGGATATCCACAATTATTTCAACATTGACTCAACTGAGCACCTT
TATCAATCTTTCATATCGACTCCCGTGTACAAGAGGCAAATCAGTGCAAAATCACCATAATACATAAAGCCAAAGCTTTCAAATATTCAACCACACCTGATATAGTCTCAAAGCATTTGAATTTCCAATTAGAGCTTGCAG
ATTAGACTTGGGAAGATCCAGCAGTTGTTGCACATTTGAGAAACCCCTTCTGCTTAGGGAACCGAAGAGATCATCAGTCATGCATGGCAACATCCATAGAGCAGTGTCACTGCCAAACCATAAACCCTGCAAATGAATCC
AAAAATCCATCAAAGTGTTGCATGAAGAGGAGAAAAATAGCAGAACCCACAAAACTAAACCTTCAAATGGAGGCTGTTTAAATCTTCTTGATGGACAGAATAAAAAGAAAGACAAGTGTAAGAACCAGGACACAGCTCCA
GGTTATTTCCAGGAAACTATTACTGCAATGGATAACATGGAACaatttATGTTCGAGAAAGAAAACCAGGTCACCTGTCCTGCAAATAAcTTTAGAAGTAGCCAATTTCCAAGGGACCCCGTGaGAAACCCTCGGCCGTCCG
ATGGTGTTTTGGACGGCCCGGATGTGTTAAaCACATTTGGGCCaTCCAAAACACGATtGGACGACCGTCGATAAGAGTGGTACATAAAGAATCGTTTATTCCCACAAGAAGATGCCTATTCAAATGGTCGAATACAGTTCC
CTAAAAGAACTTACAACTTACAAACTTTGATAATCATCCCTTAGCTGTGGAAAATAGGTCCGATTCACTTTCTTACCGAAGGGCGGGgAGGATAGATTGATTCAtCTAGCCTGTCAAGTAGAGCCCCCTATAGACGTCAAG
ACTTGACGGCCTAAATGGGTGTATCATGCCTTGGTtATTGATCCCAAGGCCCAATCCAGGATGAAAACTCCATCTATAGATCTCTAATGAGgACACAACCTTAACAcGATGGCCAAAAaTAGAAGCTTGATGCGTGAAACT
TCTGAAGAAAAAATTAACCCAAAAAGTTGATGTGTTTCGAGAAACATAAAGAAGCAATGGCTAAAATGACAAGATGATATACTGGTAGGTACAATAAACTACAGtGGGTAAATACAACCAACGAAGAGAGCCTCCAAGTTT
AGATCTCCCTATTGAATATTGGAGACCAATTCGTTGCTGCCAAGGTCAAAATTTGAAAGAGGCATAAACTAACCTAAGAATAGAACAAAACTTTTTATTTTGACTTCTGGAGCTTTGGAGGTCTAAGATAATCCCAAAGTC
CAGCGTGGTTTCTCTGGTCACAGAgaGAAATTTTTCCTAAACgaTACAAGAGATAAAGATGAcAACTATTAACTACCAACAGGGATAAAACCATGGTAAAAATACAAACTATACATAGATTTTTGGGTTATGGTTTACCCAC
cATTTTAATAAGTGCAAGTTCAGGAAAAGGTATACATGTATGTACCTACCCATTAATTCAAATTGCTGCACTGATGCGATTAACACAGAAACAGAATGTACTAGAAATCTTTCACAAGGGAAAAAAaTTTGCTTGGCCAGA
ATCGACAAAGGCAAGTCTAGTAGTACCAGCAATAACTTGGTCAATACAGAAAAATGCAACTTTTGCATCTTTTCTGAAATAATTGTACTATATTCCGCTTGTTTAGGTCTGTTTCGCTATTGGgTTGTCTTTTTGTGTTCAA
TGGAAACATAAAGCTTCATAATtCCACCCGTGGTTCCTTTCGGTCTCTTAGGAAATGGAAAGAGTTCAGCCATTTCTACAGAAACAAATATGTATTAATTTCATACTTCAACGAATGTCGCTTTAACgACATACCACAAATT
CACCGATGACTTAATTATTCTGGATTCACAATGACTGACTTAGCATCATATCGAGAAGTGCCTAGCAGTATCTGTGAAAGACCAAACATGTATTCcTGTCCTCATAGATTgttGGTACAAAACTTGAATTGATCGGGAAAGA
AGTGCCTGGTtGGAAATCATAGCAAACAAACTATAGTAACAGCCTCAAGTGGTGAAGGCATTCCCAATGTAGATCCTGAAGAaCTTATATCAGACAAATGTTGCATTAACAGTCAAACTACAAAGTTGAACATTTTGTACT
TAGTTGCATGATGGACAATATTTTACCTATCATTCGCAATGAAACTGGCAAAATCACTACATGGGCGAAATAAATAATCAACTCTTCTAACTAGTTCAATgTAATTTCAGACATCCAGCGTCCACTGTCCACTCCCAATTTT
CTATAGaTATGAATAGGATTATTTCATACGACATGATAAAAGAGGTCATCATAAAAACGACGAAAAACTCCTCATAAGAACATTCGGcAGAATGCAATATATTGTTTAAAAGCAAGCTTTTAACAACAGCAACCATGTGAT
TGATGAGACTGATGATTAAGATGGAAAAGACAGTACATACAGAATTAGAATCACCATTCTATTCTTATCTATCAGACCGTTCATCTACTGGGAAAAAGCAAAAAAGCAACGACCGGGAGAAATACCTGCATGACCATTTG
AAGAAGATGCATACAGGTGATTGTACTTGAAAGCCATCCACTGTTCGCACATATATCTATCATTGCCTGTATGATACGTATACTTTGATCGAGCACAGATTTCAAGTCTGTGACATAGTCTGTGATtGGTAATTCTAACTGA
GAAAAGTGGGCCTGCAGACCAAACATCATATATTTAAGAAGATAAGACCCCAAGAACAAACACAGAACCATGTATTATATCCAGAAGACAGTCAAAAAGTGATGTCGACCTGGAAAAGTAGATTCGCTTTAACATGTGGA
TCATCTAAACGATTCTTATCCACCATATACGGAACTTTCACAGACAATGCTTCATTGTAGTTTTCCTAGAACCAAAGAGAGGATGATTAGAGAAAAAGTCAACAGACATTTCATGTGTTATTAATATAAACTTTGAAGATGA
AGCCACGACAAACAGCCAATAAATCATATAGCCAATAAAAGAGTGCATCATCTATGTGTCCAAGAATtGGATTCATCGACTTCTCAAGCCCAAAGACTAGGGAACATCAAACATTCAGATTTTTTTAATATCATTCTTTCAT
GTGCTCTGGAAAAACTCTCTTCACAAAaCATAGATCATTACGCATAGTTGTTCAGCtcTTTtTTTTTTTtAAAaGCAACAATGTCAAGgAaGTAGCACTGTTCCTGAGTTAAAATATACACCAACACGTTAAACTGAGACAGCG
AGAAACCCTtCCTGGTTGGTGAAAGCAAAATCAGAATTTATTAGTTTGATAATTAGCAAAAAAAAAaGATGATCACAATTAGAAAACCACCTCATTATGCCTCACAGGAAGCTCATCATATTCGGAAGAACCAGACAGTAT
GTGAAGGAAAACCTGCAACGAAAAACAAATGAAGTGAAACATTGCTTAAAAAGGGATGAAAATGGAAGTTATACCGGCTATATGTTGGAGTTTTTACTTCAAGAGATGTATCCGGGCCTATATTTGAACCAAACATTGAA
ACAGTCATGTACTTCAGGTAATACTGAGAAGCTATTGAGCCCAACATCATAGGTTCCACGCTGTCTTCATTCATCTTCAAACACCCACTGTCTTCCAGATCCTCAAAGGTGTTTTGCACCAAGCTGCCAAACAAAACGGTT
ATAGAAAATTGCACTACAACAAAACCTTGGGTCATACAGTAGGTTATTACCTTGACAAATATGAACTCACAATTTCAGGTCCAGTATCCtCTAAGCCATAGTAAGCTGGATTAACCATCTGTCACAGGGATATATTTTTTGT
AAGAAACTTTAGCAGGGAAACAAAGACAGCTGAAAGCCCAaTGCTGGGTATAGAAGCTGTGGTGTGATTCCATCCTCAGTCGATCATTGTTCCAAAGATGAAAATATCATAAACATGCAcAATTGAACAcTATAAATGCAT
CTAGTCCATTATTTCAGGGAACATGACatGCATGTTTTGGGCAATCACTACAATCATTACGCTGAAAATGCAAATTGAGAAtAGCAGGTATCCAGGTCAACTTCGTGCTTATTATGAGGCATACATGGCATCATTATTTTGC
TAATAATGTTAaGCAAGAGAGTGTCTCTACGCATTGCTTAAGTTGAGACACCAAATCGAGCCTTCCACATCACcGAACTCGATAATCATTATAGACTAACAGAGTTTTAGTAATTCACTCCATACGCTCTGCTTGTTATCAT
CTACATAATTATATTAATGGGTTTGATGCCTTAAATAAATCTTTCCAAACAGCCGGGAAATAACCAATATTTTACTAGATTTTAATCTCAACTTCCAAGGGTACAAAACATTTAAAAAATATTTGGTATTGTACATGACCAA
ATAAGTACAAAACAGTTTCAAAAACTTTTCTGTTGTTTTCTCAAAATTGGGTTGTCTTTAGAGAGAAATATTGTTTGCCAAACAAGATTGGTGTTTAATGGAAACGGAAAATAGAAAAGCAAAACAAaCaaaacaGGTGATTA
GCTACCATAAGTTACAACTACTGTTCAACTTCAAAGAATTTAGCATTTCTGTTTAAATTAAAAGAATCTAATGTTAATCATGTGAAGAAACCAAAGAAGCaCTTCCAACAGCTAAACTAACACGGAAAGTTAACAACCTGG
TCATATGCATTTTACATTTTCCCCATTTTACAATGTGCTCCCACAAAAATGTGAAAACGTGTTCTTATGTAACCTCTCTTCAAGTTTCGGATACAGGACTCTGCTTGTTGACATCAACCTTGCATAGATGTTCAAAATATTT
TCTGCCATTATAAGACAAAAGAAGACCATTTTTTCTCTTCCCAAACTTTTGTATGCATCTTCCCAATATTTAAAATGTGCCTCACATCCCAAGTGTAAGTGTGTGTGTGTTTAACACTGGATGTtCTTTCCTGATAGGTGTAA
GTGAATGTAATCAACATAAATATAGTTTGACAaCCGAGAGAATGTCCAATATATCTATAGAACACAAAACAAGAACTCCAGTCTCtAGTACACGCTTTATCCTTCTGAAATGAGTATAATAAGAAAATGAGGGgAAGCCTA
GAATAAGTCAGGACCAAGCAAGGTGCTTTAAGCgCTGATTTACTTATTGCGCATAAAAAGGAATTGCcGAAATTTTACCAATCTCCGAAACAAATAAGTCCAGGTGAGATAATGAATTGCATCCTCTCTATGGCAAATTGT
TCCAGAAACTATCTCAGCATTAATGTGGTCATGCAACTGCTCCCTCAGACTACTTTCAACTGGGAATGGTTCATACAGAAACTGCAACAAATAAAAC


F3 H.png


7) FLS
submitted from scaffold near overlap sequence with query
GAGAGAGAGGgaGgTTGcTGCAATCACGCTCTAGTATCGGTtGTGAACTCTGCCGCAGGAGAGGTGCGAATGGTAGGTGACGTCagCGGCGATGCGaTGCTGGACGCcGGTGATGGGACTGGGAGGGGCTTGGGGTTTTGAAGGACCAAAGGGAGTAGACCATTAGGAGAGTtAGGTGGAGGTCC
AAGAGTGTCGTCGTTTGGGTCTGGTGGGTTGTTTCGCCATGGAGAGGTGGTGGTGGTTGGCAGTGAGAATGGGAGAGCTCAGGCGACATTATTTTTGAAGGATGTTTtAGAGGTGGTGGTTATtAATATgTGGgTTATGTAGAGGAGTGGGgACGGATACGACACAACACTCTCCGGACACG
TGTTGCTTAAATACTTGTCtAATTTTTtCTTGTATTTCTTTTATTCAAACACATGGTAGATGTTCAAGGACACGTTGGCAAAACTCATAGAGCGTGCTAAGGACACATAAAAGGTTAAAATaTAATTATTAAAACTtGGCCATAGGTGAAAATTtCCACCGATGTATAGTCATGTATACTTAtgCg
CAGACTGCTAATTTATCTTTGTTGtaTTaTTTCTAAATTAGATAGAAATAAAGATTTCTAATTTATATGAATTAATCACATACCTCTATGATACAAaTAAaTTTtATATTGGACGTATCCCCAACGTGTCCTTGTCCTAATTTTtGAAGAAATAACGTGTGTGCGTATCCGCATCGTGtcGTGTTCG
TATCCCgTATCCGTGtCcGTGCTTCTTAGACAATAAATATTGATCgTCGGTAGCCACACAGTTTACTAAGGGGCAAAATGTCTAACTAGTTGGATTCAaCTATAGCAGTAGCCGCTAAAACTCTGTAGGCTCgCTCTCTACTCCTATGGTAGCCTATGCTGCTAACTCAGTTGAtGGaTAGCAT
GGATCCcATAGAGCTCTCTCAGTCTCTAGCCTTGTTTATGGCTCAAATCCCTCCTCATCTCCAGTTTCACATCACCGGACAGCTATATAGCCAAGCCTATTGTATTTTTtGTATGAACACTATGGAATCTTTCATTCCgAaTTCAACACGGCTATCCAGACGGAACCTGAAGTGAGGCTTTAAT
CTGGGTTCTCCCATACGTACCCTTTTtATTGAGAATGCAAGGCATTTACGGATTGACATCCAAAATAAGATaCAGACATGGGACAAAGCTATGTGAATGCCGTGTtAATTAATTGGGGAGCTATTCCATTAGTATTAATTTTGTATACCACAGAAACTTCTTGCCTCTAGTtGTCCACTTTTCCC
TctcATCCTCTCATTCTGGGTGACCCTTATATGTGCATATATATAGATATCAACCCACAAACGCAAGGTTGATTAGTGATCATAGAAaGAAAAAaTGATGGACCAGAAGGAGAGAGTGCAGGCCATTGCCTCGGTGTCAACAGACACCATACCGGCAGAGTTCATCAGGCCAGAAAGTGAGC
AGCCCGGACTCACAaCCTaTGGTGGGCAGGCCcTTCAAGTCCcGGTCATCGATTTCTCAGACCCGCACGAGGAAATCCTCGTGGGTTCCATTGTCGATGCAAGTTGCcAaTGGGGAATGTTCCAAATTGTGAACCATGAGATTCCAGTAGAAGTAATAAGAAAATTGCAGCAAGTTGGgAAA
GAGTTTTTTGAGCTCCCACAAGAAGAGAAGGAGGCTTATGCTAAGCCTGTTGGGTCAAAGAGTGTGGAAGGGTATGGAACAAAGCTGCAAAAGGAAGTGGAAGGGAAGAAAGGTTGGGTTGATCATTTGTTCCATAAGATATGGCCTCcTCATACTATTAACTACCAATTTtGGCCTAAAAa
CCcACcTTCTTACAGGTTCgTGATaCATTTtCTTCTCTCTCGATTATTCgTTAGGGgCAGATAGAAGGgCAAACAaTTAGAGTACTACTGTTTTGgCAAAAAaaTGGATtATTTTTTTGTCTTTATTCAAaTAAAATTTGCATTtgTtAGTTTTGCGTTGATTTTTTGTGGATTATTagTTCATCTCGA
CAAGATGAATCTAAAAAGTCAAGAGTTACTATCAAAattaaaaaaaaaaaGTTCaCTAAAGACAATAATtCAAAATTTtCTCAAAaCACACATTCATGCGATTCGACATTtGTCATTACGCATGCGTATaTTGGGAaCATcTGATCTGCaTTTtttATTttGgTtATTTTtCTTGGTGTcTTATAAAAATGTT
GtGTCTtGTt

CV091044.png


8) LDOX submitted from scaffold AAGCTTCGTTTAATTTTTCAATATATAAGAAATGCCAAAAAATGGTACATTGAGACAGTGGAGAGGGAGAGAAATCCAACCAAAAGTTAAATAAGAAAAAATTAAAAAACCACCCAAACATTAGACACCCGAGTACCCAACGGCCTCAACCTTATCGCCTTTATCGGTGAGGTAG
AGACCGTGTCAATGCCACCGCTGCAACCCACCACCAACAATCGTTTTAAGTTGCATCCAATTATGTTTATTTCCATAAACTAGCATAAACATTTTTCAATTTTTAGTCTACCTTTTAGGTATGGAATTTTTCATGATGTTATCTTGAAGTTTGGCTCTACCGACTGACTAAAT
TTTCGTGGCTCCGCCCGCGCGCAGGTGCCAACCATTGATCTAGAAGACTTGGTATCGGAGGACAAAGAGGCCAGAGAGAGATGCCACGAGGCGTTGAAGAAGGCGGCGACAGAGTGGGGGGTGATGCACCTAGTGAACCACGGCGTCCCCGAAGAGCTGATGGACCGG
GTTAGGGTGGCCGGAGAGGGGTTCTTCAATCAGCCCGTGGAGGAGAAGGAGAAGTATGCTAATGACCATGATACTGGGAATTCTGGGAAGATACAGGGGTATGGGAGTAAACTGGCAAACAATGCCAGTGGGCAGCTGGAGTGGGAGGACTATTTCTTCCACACTGTTTA
CCCTGAGGACAAGAGGGACATGAAGATTTGGCCCAAGAACCCATCCGACTACATGTAAGTTTTTTCTGAATAACGTCCATAGATTTGCTGTTTACATTAACGGGTCCGAACATGATGTATTTAGAACCATTCTACTCCGATAAACCTGGTGGTATAGAAAGGGGCGTACAA
AAAATGTACACAGCGCGTTTGGGGACCCATTTTTGGGTTCCACATGAGTGATCCGAACCGTTCATTGTTCTAAAACATTTTTCTGAGGGGGATCAAACAAATCAGCTCAATCGGACGGTTAGAAGTGCTTGAACCAAAGTCCCACCGTTTCAAATAAAAACTTTTCATTTGA
AACGGTGGGACTTTGGTTCAAGTACTTATTATAACCATCCGATTGAGCTGATTTTTTATGGGCCCCCTCGGAAAAATGTTTTAAGAACAATGAACGGTTCGGATCACTCGTGTAGGACCCAAAAGTGGGTCCCCAAATGCGCTGTTTACATTTTCTGTACACCTCTTTCTGT
ACCGCCAGCAGCGTCCGTTCTATTCAAGTAGGAACTACAAAAATTTATTCCACTAAGACCGCACATATCTCGAGTAATTTTGAACTTAATTTGCAGTACAATTTACTAATGTTTTATGCACCG

LDOX EST.png


9) MybA1
did tBLASTn using amino acid results from scaffold03500
LGVRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG

Myba1 EST.png

10) MYCA1
submitted from scaffold 00278 (BLASTn)
tacattattttcctttctttctttCTCCTTTCTGACTTGATTGACaTAGTATTAACCGATACGATAGTATAATAGAaCAAGGGATCTACCCTGTTCCCTTCTCCTTTACGGTTCAGgAATTCATCTCCATTAGTCATGAACCCCGcTTGgCCcGCCATTGAGTCACAACTCTTCTtGTACAAACTTC
TTTCAACTgaTCAGTCGATAGGAAAGTCGGATGACTCAGATGCCTCTACTGTAAGTaGTGGGAggtGCATAATtCATTATACGGGGGaCCAAATCACAATTTtgAGGGAACATTCCTTCATAATTtATACAAACAAAACCTAAAATATAGTACCAATAATGAATAATTGGGAGg
TGTGCCTACCCGGGCCCTCGTATCCACCCTACCTGcAGGCTGAAATATTTCCTTTGAATATGTTGATGTAGTTCcCCTCAATTTTTTATTTTTATTTTTTAGAAATATCACCGTTTGGAAAATCTCAAATAAAgAGTAGTGGGATATAAAATAAGGAATCATTTCCTCTCCATA
CTCAATCCCTCCATgTCTTTCCTTACTCTTAATATTCATTCATATTtCAGGGCCAAAACAATGATCCTAGCcGTTCACATCGTAGAACACaTAGAAAAATATAGGTTTGCAAAATATCAATATGATCATCCATGATAAATTTGTGAATAAGTTACCTTTTGTTTCGTAGATTGT
AAGGTTAAACTGTAGAATATATTTGCCATTCATTTTGCATAACAATTTGTAACATGTTTACATTGTCATTTAAATTTGACTTTATATTTGAAACTTTATATTATTTTAATTAGTTAATTAATTTGTCTTAACAATGAATAATAAAAAAAaaTTcTAAAaGTGAaTAATATTACAAT
CACGTTTAAAaGTGAAAAAaTaTTACAATCAACGTTGGCAATGTTCAGCCCTAATCAATATTGCCTCCAAATTGTGGTCCCGGCACAaCGCCCAGTACGTGGAACCACAAATGAAGGCGCGTGTCATAACTTAAAGCCCATCCTTTCACTTCATCCGACACATGCTCCCACC
ActATATTGGGATGTGTGGTACAaAGTGATGAGGGTGCAAAAAAAGTTGCACGAAATGATTGCTAATACGGGCAATAGCTATTTCTTTGCTCTGTTGGACTGGAACTGGAaGGgATCTCAaGGgCAAGTGAGTATAACAACCTCCAACATTCACGTCAAATTTTaCAGCACAA
AGTTGTAACGaGATGCAATGACTACCgCcAAACTTATACAGTCCATCCAATTTGATTCTGGAGCTGTAGGTAGGAAATAACtcAGTGACAAGATATTAAaTCATTAAAAAaGAGAAACAAGATATTAGAGCATATTAAAAAGATAAAaGAGAAAaaaaTATtAATAAAAaaGaTA
AATAAATAATtATATAAAAGAAaTGAATTAAATAAAaGAATTGAATTAAAtAATTAAATGAATtAGTAaTATAAaGAAATtAATTAATATAAATAATATAAATAAAGgAGGGAAGAGGGgTAAAaGGGTAAAAGGACAACATAAAGGTGGTGGGACAACATAAAGCTTAACTGA
ATCAAGAATTTGACTGTTCTATGTTCAaaaAAAAAAAaaaGGgAaTTTGACTGTTCTAGGTAAAAATAaTGTCTTTAAGAGCTCCCAAACAAGAGAACTTATTATGAAGCTGATAAATAGTTGAATTTGCAGTCTGCCATTGCAATTCCTCCTCAACCCACCaTAAAAGCAAGT
GGGTCTTCTTCTTTTGAGCTTGAGAGCAGGATCTGATTGCAGAAATGGAAGATTAAGAAGAAACGCATCAAATCCCAGAGTGAGTTTCTTATGTGCTCTTTAACAAGATAGTCTTCTCTGAATAATCTACTTTCTTCCTATTTACTTCCCCCCCcAAAAAAAaTCTACTTTCT
TCCCTCCAAATGACTTTTTCTTGGTTACAGCCTTTTCCCCTTTTCACTGTGGTTGATGGCTTTTTCTAACCTCAACATGACAACATCAGGTGCTTATGAAAACCCTGATCTTGATTTTTTATCTTATACAAAAGCTGAACTAAAGCTACTTCAGAAATCCTAAAGTTCTCCTTT
AGAATGGCTTTTCTAGTTTTTGCTTTGCATCTTTTGACTAACTGAGTAATGGGTTTTCTTAAAATTTGGTGACtaGAGTTTTGAGGGTTATGTGTTGGGgAATGGCTAAAGGGGCACAAAACCAACAAGGGGTGCCAGAAAATCTGaGAAGGCAGCTTGCTGTTGCTGTTAGG
AGTGTGCAGTGGAGCTATGGAATTTTCTGGTCATTGTCAACCAAACAAGAAGGGTATGGTTTACCTTTTTACTATTTTATTTATCTTCATATTTTTATGGTATGTCCTTAAACATTCTATTTCTTCAGTTTCTGTTGCTGGTTTTGCATAAATTTCCAGATCTGGGAAGAATAA
CGTCTAGTTATGCTCATTATAAATGCTTCCCATTCCATAAAAAAaTTGATTTCCCTGCTCATTTATGGCCTCTTTCCTGCAAATGTTAATACGCCTGCTGATTTATGGCCTCTTTCCCTGCCCAATATTTATGTTCATgTTTGCCTAGTATGTCATTACATAATTACATTTTCTA
TTTCTTCAGTTCATGTTGCTGTTTTCTTCATTATTTTTTGATAAAATAAAATAAAACAAAATTTCGTTGagAAAGCGACGAACGAGACCAAAGCTTCCCTTACAAAGGGTGAAATGCTGAAAGGGAAgAGATTGAAAGAAAAGTAGCTTCAAAGTTCAAACCTAACACAGGA
TAATGCTTTtCTAGGGAAATCAACAATTTTCATGGGAAAGCCATTGCTTATAGCAAAGTACACCTACATGCCTTGGTACATTGAAATTCCTCATGCTTAACTGATAAAaTATGTTCCTTGTTATTTTGTTAAGGGTATTGGAGTGGGGTGATGGGTACTACAATGGGGACATA
AAGACGAGGAAGACAGTGCCGGCCATGGAACTTAAACCAGATAAAATGGGTTTGCAGAGGAGTGAGCAACTGAGACAGCTTTACGAGTGTCTCTTAGAGGGTGAAGAAAGTGATCAACAAGTGCCTTCTGCCGCACTGTCTCCAGATGATCTCACAGATGCAGAGTGGTA
TTACTTGGTTTGCATGTCCTTTGTGTTCAACCCCGGCCAAGGGTACAAGCTTTCTTCTGGACATTTTCATCTTTCTCTGTGgCTGTGAAAACTTaGTTACACTGAAAACTAAACAGAAAAAACCGAGATGTGACCGTTCAAATCTAAACAGAAAACTTAAAGTTTTAGAGTTT
CTGAACTCTGCAGAAATTCCTGTGTCCATTTTTGTTtATGAACAAGAAGTATAGTAACTTGCAAATATtATGGAGTAACCGATATTGGATCATTTGTGTGTGTTCATTAAGATAATGATCAGTTTTTGAGGAAATTGCTGTGTAGTTTGCCAGgAaGGGCATTGGCAAATGGTC
AAGCTATCTGGTTATGCAATGCTCACAGTGCTGATAGCAAAGTGTTTtCTCGctCTCTTCTAGCAAAGGTCcGTTCTTCTGGGGCTGTGCTCCGTTTTAGATTTCGTGCCAATTTTAGCTTGTTTACTAGACAAGTTTtACGATATtCAGCTCAATATACTCTCTACTAGTTAAC
TAACAGTGATGCTATAATTTTCTTCCTTTCTCAAATGGTTGGACTTGCGCACGAATCAACCTCAGAGTGCATCTATTCAGGTAAGCCGTTATTTTTATTAATTGGTACGCAAGTTTGCAAGCACACACATTCACAGGCACACGCACATAAGGACGTTGGTGGCCCCATATAA
TAGATCAAACAAGCAGTGAAGAATTTGTTTCCGTGGAGAACGTTTTTTTAGTAATTTTACTTGCAAAAGCTTTTCATAAACGAATCTTGTTGCATATACCGGTAGTCTACTACAATGACCATTGTTTtATTGCAGACTGTAATTTGTTTTCCCTATTTGGGTGGAGTCATTGAG
CTAGGCGTGACGGAGCTGGTAATTTCTGTTCTATTGCTGGATTTACCACTATTTTTGTATTGAAGATGCATCAGATGCTATACGAATTATCCCAGAAGTAACTGGTTCTGCTATTCTTTTGTTTTCcTAAGGTATTGGAAGACCCTAGTCTCATTCAGCACATCAAGACTTCT
CTATTAGAGTTTTCAAAGCCCGTTTGCTCTGAGAAATCTTCTTCTGGTCCTCCCCACAGAGACGATGACGCTGACTTCATGTATGCCAAGGTTGATCGTGAGCTGGTAAATACAATTTCGTTGGAGAGTTTCTATTCCCCAACTGAAGATATGAGATTTGATCGGGAGGGAC
TAAATGACTTTGAAAAAAATATCCAGGCCGATTTCACTATGGGTTCTCCTGACGAATGTTCGAATGGGTGTGAACACAATCACCAGACAGAAGACTCCTTCCTCCTTGATAATGTAACTTCTCAAATGGAAGATGATTTTAGCAACTGCGTTCAAGTTTCCATGAATTCTAG
TGATTGCATATCTCAGGTCgTTGTGAACCAAGAAAAGTTTATTTCTCCTCCCAAGGGTGACAATAGTAAATTGAAAGAACTTCAAGAAGGGAATGATACAAAATTTGGATCCTTGGACATTGGAACTGATGATGACTTGCACTACAAAAGAACTCTATCTGCTGTTCTGATA
AGAAATTCTCATAGGTTACTTGATAACCTTTGCTTCCATAGGTGGGATTatAAGTCTAGCTTTGTGAGTTGGAAGAGAGGAGAAATCAGAAATGGCCATAGACCACAGGTGCCGCAGCAGATGTTAAAGAAGATATTAGTTGATGTCCCTcTAATGCATtGTTTCTCACTCAA
GTCTCAACAAGGAAAtgTGGGGAAATTTtGGTTGTTAAAACAAGAAAGTTATGTGCAACATGATTTGCCGGATGATGGAATAGAGAATGAGAAGTTTTtGGtCCTCAAATCGATGGTTCCTCCTTCTGTTAGCAAGGTAATTCAACTGCCATCTATTGGAAAATATGATCGAA
TGACTCAGAATTAAATATTTCTTTACACTTCTTGCTCTCAAGACTTGCTCATTTCTATGTAGAAAATCAGGATGGCAGAAGGCATTGACTTAATCCCCACCCTCAAAACAGAAGGATTCGAATTTTCTGAAAAACAACTTTTCGATGTtGCTAATTTTAGTCACTAAAAGTAG
ATTTTCAAGAATAACATAATTTTCTTGGGGTGATGGTATACTATATTTGaGTCCTCAGCATGTCACATTACCTTCACTAATCAGAGGGAACATATAAACAACATGGAAAAACGCACAGCAAAAAAGTAGGAAACGGGAAAATCCTAATCTTATTCATCATGCTTTGCAGGTT
GACAAAGCAGCAATACTTGGTGACACTATAGAATACTTGAAAGAACTTGAGGGAAGGGTAGGAGAACTTGAATCTTGCATAGATTTAGCGGAGCATGAAGCTAGAAGAAAcTACCCAGATGAAGTAGAGCAGAAATCTGAAAACAATGAAAACAGAAGGATTGGAAATAG
CAATAAGCCTTGGATAAACAAGAGGAAGGCCTGTGATATTGATGAAACAGACCCTGAGCTCAGCAGGGTTGTTCCTAAAGATGGGCCACTGTTTGATATGAAAGTAAaGATAAAAGAGCAGGAAGTCCTGATTGAGATGAGATGCCCTTCcAGGGAaTATTTGTtGCTTGAT
ATCATTGAAGCTCTGAaCAaTCTcAACTTAGACGCTCACTCTGTTCAGTCaTCAaCCaTTGACGGCATTCtCACTGTAACCCTTcAGTCTAAGGTCTACTTCTTTCCTAaATtGTTGCaTATTTAGGtCTAAATTTGTTGAAGATATAATTAATAAAGTTGCCCcTCTCTCTAACA
GCTTAAGCTTTTAGATAAGTTGGTGGTTAACAATTCAATaTGGTATCAGAGCAGGCAATAGATCCTGTgTTCGAATCTCACTGCCtaCCTGATAATTAAATACTTGCGTGTGTTTAGCCCGCTTATGGGGgAgAGCATGGCTACACGTgAGGGGTCgTGTTGAaGATATAAAT
AATGAAGTTGTCCCTCTCTTTAACAAcTTAAGCTTTTAGATGAGTTGGTGGTTAACAATTCAATAAAATTTTAGAGACATTAATCACTTTCCTATTTCATAGTTTTGGAAAAAaTGTACTTATACTAGAAATTTTGTGCTACATATGCACTTTCATCGTTTCTGAGATGCTAGT
GATTCTTTAAAAACTTGATTTCAGTTTCGAGGAGCAGCAGTTGCATCAGCACGGATGATCAAACAAGCGCTTTCAACTGTTGCCGGAAAGTGTTGATAAACTCAAGTTTGCATATTAATGACTTCTGTCTTAGGATTTGCTTCAAAACGGTGTTGTGTAATATGTATCATTG
CAGTCCTTTCAGTCTTTGAGTTTTCTGGTCTCTTAACAATCACTTCTCATGGTTTACTGTCTAACCTAGGAGCATAAATGTGGATGTAATTTGGCTACTATCTGCACCATGTTTCAGTTTCATGTCAATGAACTACTTCGATTTTCAGCCTTGCTAGTATTTCAATCTACTCTG
GCTGATGCAATAGGAGTTCTTTGGCACATGAAGAAAGGGAAATGGTTAAGGAGAGCTAAATCTATTATGATTGTAGTTTTACAATTATGACTTCAGTTCAAACAGTTACTGAGTCTCCATTAAATTGAGATGGTAATTAACACCCTGGGGATGATGCAAGCTGGTAGTCACC
TGCCAACCATGGGTCTCAGGTAGTCATGGGTTTGACGTCCCCAAGTACTAGACGGCGAAGTTAGTTCTTCAAATTAGTCGGCCCACTGAGCCGCATATCGAGTTATAAAACAAATTGAGATGGTAATTTGAGTTGATAACCAATGCCTAAATCTGTCCTCAAGATTCTGAG
CATTAAGACGCAACTTTGTACTGTTAGCAAGATTTGGCATGCATTAGGAGTTAAACATTCAAGTTGCTTTCCACACCCCCTACCTCTTTCTGAATTAACTATTTGATCCATCATTTACTAACTATTTTAACCTTTGGGAGTTCTTGAAATTAATACCCGAGTTTCCACTATTG
ACTAAACGGATTTTCGGTATTTGCTTCAAAAaAaTTCACTGTACAAGACAAGTCATTTCTCCTAACTAAATGGACTAAATGGTGTTTGTAGTGGGAACGAGTCGTCTTTTGTCATCAGGCTGAAGATAAAAAATATATATATATAGCGTGAACAAATCACCCTTAGTTGGGAA
ATCTTTAAGCTGAAAAAaAACTGCATCATGCATAACAGGTGCAAACAGCTTATTGTGATTTCTACTAATTGTTCAAGCCATTATAGTGTCTTCATAAAAGGGCACCAACTTTTCATCTTGTCCACCAAAGCTATTAGCATGTGATATGTGCTTAAAGTTCCACAGCATAGCTG
TGTTTGGCTAATCCCTTGCAGGGACATGTTTTCATGCATTTTACACAGATTAAAGTGGGCAACGTACCTTATTAGGGACTAAAAAAaGAAAaTGTTGCTACCTATATATAGTAGATTAAATCAAAAGTGGGAACATACCTTATTAGACATGATTATGATGGAATTTGGAATTG
CACTGTACCAAAGTTTTGTTTCCAACTACTACCAACCGGTCAAAGCCTCCCAGTCTCAATTGCTGCATTTTtCAAATTACTtGTTTtCCCCATCAATTACCCACATACATATAACTCACCACATATTCACTCAATTGTGTTTGGATCATGAATTtCCGGCAAAATATGAAAAGC
GAAGAAAATAACGAAGTGAATAAGCTAAATTTATTTCGTCAACTACCACTATTATACCTTTCGTAGATCCATAATCCAAACACAGCCAAAGTATGACAACCCAGTGATTCCAAGAAACCACAAAAGAGTCTTATTCTACAACAGATGATTGATAAGAACTTCTTCTACCATG
AACAATGGCTTCTATTTACATCCACTACATTTGAAATCTAATACCAGCAAAATCTCTgACAGTAAAGCCAATTTTCTCAAACTTTCCCTTCTCATTGCTCTCTCTGAATCTCAAATAATCCGAGCAAACGAATGGCTTGTAAATCCTCGAATTTCCCTCTCCTACCACTTCAT
CAGGTGCCAAGATCACCTTATCATTCTCAAAGCACCAAAAGAAAGCAAGCGAAAACCGGTTCAGTGGTTTTTtCAAAACAACTCTGTGTTCTGATGACCTTAACTTCTCATTGCTCCAAGCTTGAAGCAAATCACCAATATTGACcACAAGGGTCCCCTCACATGGACTAaT
GTCAATCCAATGGCCTTGATTTGATCTCACCTGGAGCCCACCCATTTCATCTTGATACAAAATTGTTAGACAACTCATATCAGTATGCATCCCAAGACCCTCAACCTCTTCTTCCAAACTCTTTGGAGCTGAGTAGTTAATTATCCTCAAATAACCATGGCAGTTTTTGTACT
CAGAATCATAATACTTTCTTTCAACATCATCACCCAAGGTCATCAACACAATCTTCACAATTTTCTTCGATAAATCTGTCATTGCGCTTCCATATtGTCGCAATATTTCACTAACCAAAAGAACAAAAAAaGGAAGAAAGAAAACTGTCAACGAAATTTGAACGGAAACAGAT
GAAGGGATGTTAGGTTGGACTTTTCAATTTATGCACAAAGTGTCTGCTCTTTGAAACTTTTACCACTGAAATAGCAGCCCCTTGTACCCTTCAAATATTGAGGGACTCAAGGGACGTTAGGTTGGACTTTTCAGAAGCTAAGAAATATAATTGACGAGCTGAAAAAACTTCG
CTGAAGACGAAAAGATTCTGAACAATTTTGTACTTAAACATCCCCAAAGGAGTTTTAGCCAGATAAGGTGAGTACGTAGATAGACAATAGCATTACCTGAACTCAGAATAGTGCTTGTCAAAGAGAACATCTGCTGAACTCTGAGCAGAGCCGGTGAAGTCTGGTCCAGAA
ACTCGGAGGCTCTCAAAAAAaGGAGATGCTATGTAATGAGGAGTGTAGGTTTTGATAGACGAGAAAGGACCGAGTTTGATTTtCGTCTCATCAGGAAGACTGAAGAGGAATTCTGAAAGTAAGCGGATTTTATCATAAAGGTCTTTCGAAATCCCATGATTGGTGATGTGGA
AGAAACCCAACTCTTTGCAGGCTTCAGAAaGAGAGGACAAAGAACTAGAACATGGCTGCAATGGCTGAGAGATGTCTAaTATGGGtAGTTGTATGCacaCCTTGGACACCATTTTctATATACAGGAAAGGCTTGGTTTgTCCAtcTAGGTTGGGGCTATTATATATAAATCAA
CTGATAAAACAACTTTtGACACTACCATAATATtATtATACAGAGGCCAGAGTAtGGTAGAAaTAGAGATTCACTGTATagCATAGTTGCTTAGGAAGTCTTtGTAAaCAAACTTTCATGAGACTTGTGAGATTAAACATTAAAGGCGTAATTATCTTCGCTTGCCATAAAATGG
TATAATAGTAGCCAATTTTTCGCCATTTGTACCTCAACCGTTCcTACTTTCGTTTtCAACTAGCGTACATTCAAAGAGGAAAGAAGAGGGGAAAGGCATCCCATTATTGGTGATGTGGAAGAAACCCAATTCTTTGCAGGCTTCAGAAAGAGAGGACAAAGAACTAGAAGAT
GGTTACAATGGCTGAGAGATGTCTATTATGGGTTAgTTGTATGCATGCCTTGGACAACATTTtCCTATGTACaGAAAACGCTTGGTTtGTCCACCTAGGTTtGGgCCATCATATATCCTAtATGGCTATTCTAGTCTTTtGAAAATGAAAGAGagaTATATGAaTGTcTATGAATC
AACTGTCCATTTAACCCTcTTTTTtAGAACCctATGATCATTCAAATACtGTCATCAAGCATGTACAGTTCagTAACTCCCAAACTACCATTAAAACTATAAGGACCTTTAGGTAGTGTAACCGACAAGGATTGAGCTCTCTTTCGATGCTCTCAGATTGATCTAGTgaGGAATA
GAATTGAGTAtGATGTACATTTCATATGTGCTATcGATATCCTCATCATATATGCAAAAAATACTGtATGTACGACGATAATGTTGtACGTTGATGTCTATtGAGCTTTTACCaTCATAAGAGTAcAATTTTACATGAAATATCCTGTGATAAACAACTTTTGACAGTACCATAA
TATTATTAATAGAGGtCAGAGTATGGTAGAATAGGGATTCACCGTATAGCATAGTTGCTTAGGAATTCTTTGCAAACAAAACTTTCATAAGAcTtGTGAGACTGAACATCAAAGGCGTAATTGTCTTCACGTGCGATAACAAGGGTATAAAAGTaGCCAAaTTTtCGCTGTTTG
TACCtcAACCTTTTCTACTTtCCTTTtCAACTGGTgtACATTCAAAGCGGAAAAAAGaGAGGAAAGGCATCCAATGCAAAATaCCCGAAGTTATCaTTTTTCAagCAATGCACAGctCTAAACTGAAGTATTAACTTTGCCTAGTCCATGTCAAAACATGAACCAACCCAACACC
CCTtATtcGGTGCAGCTTAGGTAATTATTGAGCCCAGAAGAAAGCTAaTGGACGTTAGAGTCCCCCACAAAAAGGCAGATAAGGGGGGAAAAAGAAAACAACATTGGAGACTAAaCAAATCTCAATGGGTAACACTCTTATTAGACAAAACTGCTGTAAAAAGTTCATTAAT
GAGTTCTGGAACATTtACACACCAACGCCACAACAACCCGAATTACCCGGTTTTCTAATGGAAACAGACAGAGTAATGATTTGATGCATTTTTtGAtGAAAAaTtCTACAATCAGCCCAATGGGCTATCGTTAAGGTTTTAAAAGTGGGGACTTTTCCGCGTAAGAATCTTCA
AAAAGTGGGAACTCTTGCACGCAAGAGTTAACTCTTTCATGCAAAGGTCTTACCTTATTGTCAATCTAGTGGGGACAGAAGGATTGTTTTTTTtATTtGCGGCCACTACACGTTACTCGGCCAAATaTATAGGAAAATATGACCTGCTCCTTTTCTAAATGCAGGGCACATTT
TTtGTTCTGAAAAGGAATCTGcGGAGGTAGGTGATTTGGCTCcCATATAACTGTGTAGGAAAGGTGGTTGACAGGTTGAGTAGTTtGTATCTTTCTTCCGGTATTGAGTACCGACTCAACACGGGAAAACTTAAGAcgTCTAGACATAGTAACCATTGACAAACTGAGAGCTC
TTTCTTGATTCTATGGGTGGTGGACCGTTGGTgTtGTTGGTTCGAAAAaCCGCACCCCCTTAAATCTTGGAAGgAGTTCAGTGAATCATATtGTTAGCCGTTTTGGAAGAGTGTTTGAAGcGTTGGAAATTTACGAAATTTCGGGTGAATAAAATCGGGACATTTGGAGGGTC
TCACGAGAAAACTGAAAAATCCTCTCCAGATTCCTTGAATCTGGACAAGGCAAAAGTTATGGGGCTGGTCCCATGATGTGTGGTAACGTCTCAAAATTATTTCAGCGGTTTCAAAATTAATTCTCACCTAGCAACTCAAGGCTTTCTCCTTGTGTATATTATGGAATCCCGT
TTCTATAGCCTGGCCCAGGACCATAAATTCGAATTCCATAAGACATATTTTGTTTTTCGTTCATTATGTATTTATGTGAGAGACACACGCAAGTTTAGTTCGCCACGGCTGTTTTCATTGGTGCACTGTGATTTTAGCCGTTTCATTCTGGGATGTAGACTTGTTGGGTATGC
AGACAACTTTCAACAATAGCCCGGGGTGAAGGAGGCAACGCCACCACGGCAGTTGGCACAAAAGTTTGTGCCGCAATCCATGTGCAACCCTAAGAGTAcACGTATTGCGGCGAAAACGGTATTTTGTAATTGGGCTAGAGCAATTTCGGCCACCTATTGCAATTGCTTTGT
AATTAGGATTCGAGTGTTTTCAGATTAAACACGAGATGAgAaGAGAACTTTTTtGAGAGAGAAGAGAGGACTTGTAGAGTTATACTCCATATTAGAACCCcAAAACGGGTAAGGGTTTGGGTAAAGAGTAAAGATTGATTTTCGATAATTTtACCCAAATTTtGAGTTTGGAT
GTTAAAATGGGTCAAAATTGGAGATGCTTTTAGAGATACTGGATTTTGATTTTTAAATTTATCACTTGTTGTTTCTCCACAAGGAATTGATTTTGCACTCAATTTTTTTttATAGATGCaCTCCAAAACTTGTCTTTTATTATTtCAAaTTACATGTAACAAATTTtCAAAGCACA
CTTAAAacAaGTGTACTAGTTCTTTTGTTcGTTTAAAaGGgAAAAAAAAAaTaCTACAGTAATATTCAATGGAATaTTAGTCAATTCGGTTTCCCACCGGTGCAAACCAGTATTTGAGCCGGAACCTACTGCTAAACACGCTTGAGAGATCAACGACGTCGGGTGGGTTAGGG
AAAAAAAAAaCCTCCAAtACGTCGCAGAAGGGACCTTGTTTGAATTACGGCGGGACTTGCAACTCTCTCTCTCTCTCATGAGCTTTCTCTGTCGTCTATCGTTTTCCTCTATATATACATAGATGTGCCCTCTTGTTCGGAAAGCATACAACCTGTTCGAAGAAATAgCCCAA
AGGTGCCCTTTTGTGCGAAACTCTGTACGTTGTTTATCAAACTGTGCGTTGAGTCAAACCCATTCCTATGAAGACCCAATTCAATTTTCTTATGAAACTCAAAATGTAATATCTTGGACCTCAAAAATCTCCGCTTTAGTGAGGAGAGAGAAGCCCCGAGAGGCCATTGGGT
TGTTCAAAACCATGCTTGTGAAAGAGCAAAGGCCAAATTATGTGACAATTCTGAGCATCATACGCGCTGTTGGCAGTTTGGGTTCTGAAAATATGACCCAGGCAATTCATGGGTTGTTGTTCAAAATGGGGTTTGAATCAGAAATTCCAGTCGTAACCGCCCTTTTGGGTG
TTTACTCTATGTGGGATGTTGAAGTTGTATGGAAGCTGTTTGATCAAATACCCAATAGGGATATGGTTTtATGGAATGCAATGGTTTCGGTGTGCGCgAaGAGCGGGCAATACAGGGAGGCGTTTGAGATTTTTAGAGAAATGCAGTACGATGGTGTTAAACCAAAAGATGC
CAGCATTGTAAGCATACTGCCTGCTTGTGCTGCTCTTGCTAATTTGTCCTTGGGCAGACAAATACATGGTTTCTCTGTAAGAAGTTTATGTTGTTCACTTACAAATGTTCAAAACTCACTTGTAGATATGTATGCGAAATGTGGAAATCTTGAGACCTCTATTCAAATATTCA
ATAGAATTGAAAAGAAGGATCTTGTTTCTTGGAGGACCATGCTTCATGGGTGTATCGCCAATGATTGCCCTCGAACGGCACTGAATATGGTCTCTAGAATGCAATCTAATTGCTTTAGACCAGATGAACTTTTGATCCTTGATGCAATTGGAGTCTCTACACAAGAAGAAAA
ACTTAGTTTTGGGCTGGGATTTCATGGTTATATCGTAAAAaGTGGTTTCTTGGATTTTGTCTCGGTTGTGACTGCACTTCTCCAAaTGTATGCTAGGTTTGGTGAATTTGGTTCAGCTGGGGTTTTATTTAATCATATCACTCAGAAAGATGTTATTGCGTGGAGTTCCATGgT
ATCGGCATATGCGCAGGGTGGACATCCTTtGAATGCTTTAGAAACTTTCAAAAAGATGCAATCAACAGATGAGAAGCCGAATGAGATCACTCTAGTCAGTGTATTACAAGCTTGCTCTtCTTTGGGAGTCCAAGAACTTGCGGAGAGTATCCATGCGCATGTGACTAAAGCT
GGCTACTCTTCCAACACAATATTACTACCAGCATTAATTGATATATATTGCAAATTCGGAAGGATAAGGCAAGGAAAGGCACTTTTTGATGAAATTCTTATCAAAGATCTTGTCTGTTGGAGCTCAATGATCAATGGTTATGCGATAAATGGGTGCGGAATTGAGGCTCTGG
AGACCTTTTCAGAAATGTTGAAGTTTGGAATAAAGCCCAATGAAGTTGTCTTCATTTCTCTTCTATCTGCTTGCAGTCATTGTGGGCTAGAAAATGAAGGCTGGACATGGTTTTATGCAATGGAGAAGGTATATGGTATTACTCCAAAACTTGCACActATGCTTGCATGGTG
GATCTGCTTAGTCGTCAAGGACATgTAGAAGAAGCCCTTGAATTTGTGAAAAGTATGCCTATTGAGCCTGATAAAaGAATTTGGGGTGCTTTACTTGCTGGTTGTAGATCAATTCATGGCtCTATTGAGATAGCAGAATTTGCGGTGGAACGGCTCATTGATTTGGACCCGG
AAAaTACTAGCTGTTACGTGGTTTTGTCAAATTTGTATGCAGAGCAGTGTAGATGGGAAGACGTGGAGAGACTGCGGGAACTGGTAGACAATAAGGGATTGAGGAAGGAAATAGGATATAGCATTGTTGAAGCCAATTCATAGCATGCATATTGTGAGATTGGTTAGCTGT
GTTCTCTCAACAGGTTTCTTCTTGTTTTTtGTTtGACTATCTGTAGAATGCTGAAAATAAGTTGCTGGATATATCTCTTACTCAGAGATCATACATCCATTTTCAATCTCACCTTTTGGTTTGTCCGTACATCACACTACATACCTTAGACAGAACTTCTTTGAATTTTCCAGTA
ACGTGAATTTAAATAGGCGTCTGTGAATTTAAATAGCcaTCTATGTATTTATTGTTGCGGAGTTACATTTAGTAGCGGCTTAAGTAAAGAATTTATTGCTCATTGAAAGCAGTACTGTATGCTTCCTTTTATATCCTCCATTAATGCGTATTTCACTAAGTTAACTTTCCAGAC
CCTTGTTACTAGTCCAATAAATTCTCGAGATCTTTTCCATATAAGCTTTGATTTATATCCCGTTCTGATGAAAGAAGGAATCCTTGAAGTACTTCTTGTCAGCGCTAAAGACATTAGAAACACCAATGTTATCGGTACTCTGTCTCTCTCgCAGCTAATGTCTAAagAGGCCA
TGTGTATATAATATTTACACGGATTATAGAAAGCACTTTGTTTCTTTGTGTGGCATTTTTTGCTTTCTCTTTTGCATTAATTGGAGCTTGTTAATTGTTCGAACTCAACCTCGTGTTTAACAGGGAGACCAGCATATTATGTGCTCATTGAATGTGGAACTCAAGTGGAAAGA
ACCAAAACTACAACAGGTCAAATTCTTGGTGATAGTTCATCCATCTTCCTAGCTCTCATCCTAATATGCTTTCCCTAACCTAATTACAGAGTACATTGGTGCAGGTAATCACAAAGAAATATGCTGGAATGAAAAGTTTGCGTTTGAATTTCCCTCTTTCGAATGGGAAAAC
TTGACTCACCTCAAATTTAAAGTCATGGCTGAGGAATATTTGTTTGAAGGAGGATTTGTTGGTGAaaCCATGTAAGCAGTTTTAACTCAAAACATTATTCTTGTTCAGTTTTCTTTGTTAATTACCAGCCATATAATGCTTTCATTTTCTTTGTTAAACAAACAATTTTGTACT
TTCTGCTAGGaTAAATaTCaCAAATCTTCTAGAGCTTCTTTTAtGTcAAACCCaGCAAGGAACTTGCTCTCTCAAACCATCTTCTACCCTAGTAGTATTGAATATTGATCACACGAAGTTAAGAAAAAaGGGTTTAATTTCTGCATtAATTTTTtGTTTTTtGAAATCTTGTGTAT
GCATTAAGACATTTACTTTGGCATTGGAAACCTTAtGAGTTATGAAGGAATGAATATGTCTTAGAGGAACCGTTGTCTCTCCCTCAATTTCTGTATTTCTATAGGTTAAACTCCTCGTGATGATTATGAACAATGTGATTTGAAGCTTTGAGAGCTTTTAACTAGTAAAGCAG
TTCATGAAATAATGGAAGTACTAGCTATAATATCTCcTCATTTGCGTATACATACATTTTGTTTTtGTTGAAATAATTTCTGTATCCCAGATTCtcGGGgCAAATTAACTATCTAGATATTG

Got:

00278.png


11B) MYCA1 scaffold 00554
Submitted from SSR primer area

GAAAACTACtAaGAAACATTGTCACAAATGCAGAGGAACACAAACTcTTTCCAAACCAAATGAAGAAACTGAGTTACGTACGCCCAATGTAAACATTATGACTTTTTACtaCTAACCCTAGTGCAAATTATTGCACTATTGTTGCATTATGAAATTCTAAACTATCACAAGAATAATGAAGGGGTTTtATAGGCTA
ACCcTAacAACCTTAGCAAAATCCAAATAACCCAAAAGGACTAAAATACCCTGAACGTAAAAcaaaaaTAACGATAACCTTACAGAAACCcTAATGACACAAAAAGACTAAACTACCAtGAACATAAACAATAATCTGAAACCCTAAAttACACTCCCCCCCCcTCCTCcTtCAAACGCAGGATGCGAAGCCG
GAGAGCATTGTGAGTTTGCCAAGGGATTAACAACAGCGAATCAACTGCTGTGATGGTGCTGAACAATGCAAAGCaGTACAAAACTAGAATtTGCGAATAGGAAGAAGAACTGATGCCGAATAGATGCAAATAGGGGAAACCAATGCCAAACAGATACCAACCAGATGTCAAATTGCtGATGCAAACAAT
ACCAAGAGAGAGAAGAGAACAGAAgCAAAGTGTCAGATCAATTAAGATGCAAAGATCGACAGAGgTGTAGGGAGGAAGAACAGATGCAgAGAGATGCAAAAACTGATGCAGATTTAAGAGGTTCAAAAGTTTAGAGGTTCAAAGGTCCAACATAGCAGAATATTGCAGAATCAGAAGCCCAAGTAAGA
GCGCAAAATACCATAGTGGTTGTAGAAGcGGAGGTCGATATAATGTGCACCAGTCAATCGAAAGAAACCCACTCAATTGATGCAATCTGTCGATTCCAAGTGCATTAGGGAgaGACCCAGTCAACGTCGTGGACCAACCCAATCAGTTTAGAAGTCAATCAGAAgTtACCCAGTAAATTTAGGTAGAACT
ACCCAAAAGATTATGAACATGATCTGAAGTCATCGTGGCCaGaCTGGAGATTGGTTCGCCCAAGGTCGTCGGAGCAAGTCAcTGGGTGGgAGGTGGCTGCTGCTGCTATTGTGTTGTTGTAcGTGATGTTGCTGCTGGTGCGATGAACAGTTCATgTGAATGCCCCGcGAATGGTGGCAAGCCTTAGGcT
gCCcGCCGGCGAGtGAATATCCGACAtgCCTAGATCTTATTGCTCTGATACCATGTGACTTTGCAAAGAaGGAAAACAAAACTAACGTATTATtGCaTTATGAAAGTCTAAACTATTACAAGAATAATGAAGGGTTTtATAGTCTAACCcTAAAAaCCTTAGCAAAaCccTAATagCCCAAAaGAAAaTACCTG
GACATAAAAGATAAATAAcGATAATCTTActGAAACCCTAATGACACAAAAAGACTAAACTAcAATGAAAATAAACAATAAACAAAAAGACTAAATCAAAGGTTGGTAACTTTGCATGTAAGGGACAACCCACAACTCTACTAAAAAaGGGGGACAAACCATAGATTTCTTTTCAATAACAGCTTtCTTGG
AGAGAGAGAGAGAGAaGCATACcTGTAATAAtCCTTTTAGGTCCAAGCTCCCTCACAAAATCAAGTAGTCTAGACAATGCATCAGGAGTACGAGCATAATCCAcAATCACCCCAAATGCTTGTCCCTCATCTATCAACTCACACCTACCAGGAACTGCATCAACCTCTtCAATCCcTCTAACAATATCCTC
CAAAGGTGCTCCAACCGCAATTCCAACTGCAaCAGCTGCAAGAATGTTGTATACATTATGTCTGCCAAGCAACCCCGAAGAAATCTCCAATAAACCGTCGGGCGTTTTTACCAAGACCTGGGTCTCAAATAGAGATAGTTCAAATTTCAAAGAATGAACGTCAGCatCTTTATTCTCCACTGCATACGTCA
CAACAGGCACATCTGGGTTgCCTTGTGCAATAAAGGAaGCTGCATTCGGATCATCAAaGTTGACGATTTTCCTGTGTCTTTctGGGTCCACCATTCTTGAAAACaGCTTGACCTTAGCATCCCTATaCTCTTCCTCagaCCcTTCAAAaTCGTAACGgtCCCtGTCAAATTTGTGAAAACGgCAATGTCAAAGT
CAACCTCATTGCACCTCCCTAATGCTAGTCCATGTGACGAAGCCTCCATCACAACTGCTTCTGTCCCATTATGCACCATTTTCGCCagCAACTTCTGAACCAAAACAGCATCTGGAGtcGTACTTGGCGACTGCAACTTaTTATCTCCATGAATATAATACGCCACGGAGCTGAACATCCCAGTTCTCAAT
CCCATGGCTTCATACAACCCTTTAATCAAATACGCGGTGGTAGTCTTCCCATTTGTACCAGTAATCCCTATCACGGACATTTTCGTAgAGGGGTAaCCAAAAAAAGCTGCCGCCAATGCTGGgAGCACCGAACTTGTGTCTTCCACAATAACCACAGCCTTACAACCTAATGTCTCCTCTAAATCTATCTC
TTTACTAGTTACCACAGCAACTGCACCTCTCTTGTATGCCTCCGTCAGATACAAATGCCCATCAGTTTCCCTtCcGACACAACACACAAaCAAATCACCAGCCTCAACAAACCTCGAATCATGTTGAATCCCACTAATCTCTACCTCTAAATTACCATAAACTGAAACAGGTACTACTTTACTCTCATCTAA
AAGCTCAGCTAATGTCATATAAAACGTGGGGTCAACAATCCTAAACTTGTGACTTTTATCAATCTTATCGAAATCAATATCGAACGATGAATCAGAACTCAAATCAGACTTaCCCAAATCAGAACTCACCTCATTCTCCAATTTCTCACCCTCTTCATCATCACCCGCATCTGCCGGAATCACAGTGAGCC
CTCGCAGCTCCTCAATTTCCTCCAAATCAACCACTTCTTCAGGCTCCAGCTCATCAATACCCTCATCCACTTCCTCAACCTTCTTCTCTTTCCTAGGGTTAGGTTTCAACAAaCCCTTTTCAACAAACTCTTTCCTTGACAAAGCAATAGCCTTATCAATATCAGCAAACACATCATCCCCCGAATCTGTTG
GAAAAATATTTTAGAATTTCTGTAAACcAAAAAAaCCAGGGACAACAaTACAActATAGCTACCATAAACATTTGTGGCTACGGGAGTTACATTTGAAATTTGTAACTCTTTTACAACTTTCAATTAGCAAATCATTACAATTAAGTACAATTACAAAGCTATCACTGTTGTAAAATCAAATAATAGGTACC
ATGAAAATAAGAGAGAGTTCACTCTTGCCTTGGGcgAGGATAACTTTatCCACATTCGGCGGGgTTTTGTGGGTTCCGATTCGGTAATCAGAACCATTcATTTCGTATGACTTGATTTATCCACATTCGgCGGGGTTTTgTGGGTTCcGATTCgTTAATCAGAACCATTTATTTCGTATTACTTGATTTATTA
GGAGACcaTGTGAAAaTTCAGCTTAATCATAtAGTTGTAAGCACTCAaTCCAAAATTttAaTTTTtCTCCATCCagTTCTAAAGCTAaGGAATTaGACTTtCTATGCTCAaTATctAATTGATCTAAATTTTTTTGCGTTGTCTCCTAACAAATCAATTCATCCAAAaTAAGCAGTTCCAATTCCCATGGGTAAAAT
TACACTAGCTCTGTTGAACAGAATTGCTCGCTACAAACAATCAAATAAAATTCAAATCACAAAAAATCTAACAGAATCACCACCTCGGTCTTCGAACCCGGGATCGTCGGGTGCGTCCTCCTCGTCGGCAATTGCATTGAGAAAAATTGATTGCTCCTTTTCAGACCGCTCTTCTTGGATCTTTCTTGCT
TTATCAGCATGGCGCTTGACTTGCTGGAACCTGGATACCCCGTGCATGGAGTCTTCTTCCGCTTCGGGCGGGTCATCGTCGGATGGGTCCGGTTGGAATACGCCGTcGGGgCCGATTGCGGCAGGGGGTCGGAGTTGGAGTTGGGGTTTtAGGgTTtGGTGAGgAaGAGaGAGGAaaGGgAaGGgTTtGAa
TtGGGgAAAAaaTGTtGGATTTTtCAGAGAGGGGGTGAAGAGTGGgAAGGAGATgAAGGTGGGTGGCAATGGCATTGTAGAGAGAGATTAGAGAGAGAGAGAGAgNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAACTCAATTCTTGAGGCCGCA
AACaCAATTCATGAGGCCTCGAACTCAGACTTTGTGGCCGAAaTGCTTTGGCTAAAAAAaaaCATTTTtttCTTtGGTCTTCATTAAAATAAAAaTTTGCATtcGTTAGTTTTGCGACGATTTTTGTGGTCTATTGGTTCGTtCcGACGAGATGAATCTAAAgAGtaTAAAAttAAAATTTTtaaagaTTTCACTAAAG
ACAAAAaTAATCTAGATTTTTgTgATTtATTAGATTgTTTTtGTCTTTATTCaCAAAAATTTAAGTTtCGTTCATAATTTTAtACTTTTTtAGATTCCTCTCGtGgAGAGAATCCAATAAATCACAAAAAAaTCGATGCAAAACTAGCAAAAACAAATTtCTTTTGAaTTAAAAAAAAaCATTTtAGTTTTTTtAGCC
AAAGCAAGTGAACGAAACTTGAATCTTGTGACCTGAAACTcccACCTTGTGACCACTAACTCGAACCTTTTGGCCTCAAACTCCAACCCTTtGGGCCGACAAAATGTtATTtCTCATCTTAAAAActtAGGCCTaTTTTGGCCAAAGTAGAAAATCAAGCTTTtCcTTTtAgCTTTTTTTTTTttGGgAAAAAAaCT
CAAAGGTAAAaGGTGGATtGTTTtGGCcTTTTTTttGTGCTTTTTGCTTTTCACCTTTCAAATTGTAATtGTTTTTTtATtACTTTTtGCTTATtGTAAaGTTACTTCTTTAAGCCAAATTCTATGACTTTtCgAaTCtCAAAAAAGCAAAAGGCTTGCTTTTCTAGTTTGGCCAAAATAAGCcTTtATCTCCATAAtttg
ttGACTACTTTGTtATTCATGATTTTTAaTCTTTAGtgATTTCTGTCAAATATTTtATTTTTTCCCTTTTTAGATGCATTCATACCTCTgTTTtAAAAAATATAAAAAaaaTTATGATATTAAGAAAAAaGACTAAAAaCgAAAAaTAGGCAACTATATACAAGCCTTTTtAAtCtAaaTAAAAAaCTCATCCAAaTAA
ATGCTACTCTAACTTGTTTTtCTCAACACTTTTGTCAACAACGTTTAAaTAATCTTGTGAAATGGAAATGAAAACTATTTTAACGCTCCTTTTTTTCTTTCTATCTATTGgCACTCAATTTCTTATCTTTTATtgCGTGACAAAaCTAAACTATCCTTGgTtCTTATCTCTTGAGTCAATTTTTtCATATAAAAGG
TAaTTCGAtACTtCCGCATGAATAAAAACCAAAAAaTGGGTGCCAATGGATAAAAAaTTaGCGTTAAAatCATTTCCCAAAATAAAaaTaGTTgCGTCAAGGTTCTAAAtaCCATAtCGTTTTTTCtAGTTGTACAATATGAACCAGtNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGCACTATGCTGCACCCTCCTCagATATTTTTGGTCagCTTCCCGCCAAACCCACTAGgCTAAAACCCTAGTTGCCTTTTCCCC
CACTCCAaAACCCTAGTTCtCTCTCTCTCCCTCCGCCACCAGTTCCCCACCCcACCACAACCAACCATGGACCCTCAGTCcGTTCAGACAAACCTAGTCCTCATCCTAGACTTCGGTtCTCAGTACACCCACCTCATCACgCGACGCATCCGATCCCTCTCCGTCTTCTCCCTCTGCATCTCAGGCACCTC
CCCTCTCAAATCCATCACAGACCtAAaCCCAAGTGTCATAATCCTCTCCGGTGGTCCCCACTCGGTtCATgCGCCCAACGCGCCGTGTTTCCCtGATGGGTTCATAGAGTATGTGGAGTCTAAGGGCGTGTTTGTTTtGGGCATATGTTATGGGTTGCAGCTGATTGTGGAGAGGCTGGGTGGGTGTGTtGG
GgTtGGCGAGAAGCAGGAGTATGGGAGGATGGAGATTGAGGTGGAGAAGGCTTGTGGTTTGTTTGGTGGGTCAAAGAAAGTTGGTGATAAGCAGGTTGTTTGGATGAGTCACGGGGATGAGGCTGTGAAGTTGCCTGATGGGTTTGAAGTGGTGGCGCGGAGTCAGCAGGGGtcGGTGGCGGCTGTGG
AGTGTCCTGGGAGAAGGTTTTATGGCCTTCAGTATCATCCGGAGGTAATTGGGTATCTTCATTCATTTCATTCTTGGCCTTGTGATACGCTGCCCTGTTTTAATTGTCTTTGTAATTTGGTtCTTTGGCTGGCTTTGTTTGGTTCTTTACTAGTGGTTATTGTTTGTACGCGAAGTGCTTACTTGCTGATATC
ATCTTCATGTTGAAGTGGTAAtAATGAATGAAAAATGGAATGAATTGACCCAATATCTGAAAAGTTATCGTCTTTAGTGGTACTGTGGTAGTGGCCTATAAAACTGTTAGCGAcACTCCCTCGtGATGTATCCTGCTCAGGCCcAGATCCTTAGCAAGAATGAGTCAATGACCATTGATCCCTTTtCAAGCA
CTTAAGCtgAaCTTCCACATGgTACCATAAAATCCCTGAATCCTCTCAGCACAAGCACTGATGATCTCTCTGTTTGAGTTTCACATCACTAGCCCAACCCCCAGAAAACcATCCTTATAGTTATAGTAGCCCCGTCAAACTGAATTGTGAGCTCACCTTCATTTGGAGCTTTCCATGAGCCAGCAAGATGTT
GGGATAGTACTACAGTCTTTGGAAATACAATGGGAGTTCTCTCAGGTAGCTAGCTGCATATTCtGCAAGATCACTGGCTCCCCTGTTTGTCCCtccAAAAAaTGTAATTGCCTATTTAACCAAATTACCCAAGCCACAATTAGAAagAGATCTATTATTtGTTGGAACCAACCCCCTCAATCTGCATAGCCCA
GAGATGCCCCTTTCTTCAAGCACATGCAAATCTTTGAATCGATAACCTTCCGTAAcATTCTTTGCTTACTCACaCtCTTTTAGACAACGAATGATGGTCTCCGGTGCGGCGCTGTCCCTCCACACAGGCTACAACAATCCTTAATATTAACCATTTTTTtGACAAGCTTATGGTTCATTGGGAAAATCCCCA
AACACAtCTTTCAcacaatgaCACAAACAATTTACTTTgTGTTGGgAaTTTTCAGATTCCAGATTTCTCCTAAGATCTcATATTATCCATGTCAGAAGATGAGAGTTGAGAGTgCTGAAAATGTAATGTCCCTTGTGGTGTAACCAGACtCcATGGTGTAAGTTTCTTGTTTAGTGAAGTTCCATGTTTAGTTGT
CTTCATTTCCCAGGaTAATAGGGGAATGTTTCATGtCcTATgAGCTTCAAAAGGGaTAAAaGATCACAGCTAAGATGTTCCTTCCAAACCCCAGTATTGTGGTCAACGAGGCCCTTtACTaCCATTCTCTCCGTATCAGGCTTACAaGAGCTTGTAGTTTTAaGGGAGAAGTGCTTGGgATCCATtGGTCTGG
CCACACCCTGGTTTtAtATCCcTTTCcGATCCtCcACCTAAGaCCCTTTTTAAGCACCTCCATACCCTCACACAT

00554.png


12) UFGT
search with amino acids using tBLASTn
SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL

UFGT EST.png

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