Difference between revisions of "Prepare PCR product for sequencing"

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# Pipet 4 µL of sequencing primer (at 6 µM) into the same bar coded tube.
 
# Pipet 4 µL of sequencing primer (at 6 µM) into the same bar coded tube.
 
# Place the small cap back onto the bar coded sequencing tube.  
 
# Place the small cap back onto the bar coded sequencing tube.  
# Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab.
+
# Record the bar code number of your tube. Write your name next to the bar code number on the sheet in lab.
 
# The information will be submitted to the sequencing company [http://www.operon.com/default.aspx MWG Operon].
 
# The information will be submitted to the sequencing company [http://www.operon.com/default.aspx MWG Operon].

Latest revision as of 20:15, 9 October 2013

You have amplified your TAS2R38 alleles for sequencing. Now you need to mix the sequencing primer with your PCR product.

  1. Pipet 8 µL of PCR product into the bar coded tube.
  2. Pipet 4 µL of sequencing primer (at 6 µM) into the same bar coded tube.
  3. Place the small cap back onto the bar coded sequencing tube.
  4. Record the bar code number of your tube. Write your name next to the bar code number on the sheet in lab.
  5. The information will be submitted to the sequencing company MWG Operon.