Difference between revisions of "Test pLasR/cI"
From GcatWiki
(4 intermediate revisions by the same user not shown) | |||
Line 15: | Line 15: | ||
| 1 || 1 || 0 || 0 | | 1 || 1 || 0 || 0 | ||
|} | |} | ||
+ | |||
+ | |||
+ | '''Test pLasR/cI:''' | ||
+ | |||
+ | pBAD→RBS+LasR+TT (from James DC) | ||
+ | |||
+ | I13453+B0034+C0079+B0015 | ||
+ | |||
+ | plasR/cI→RBS+GFP+tt | ||
+ | |||
+ | K09110?+E0240 does not glow | ||
+ | |||
+ | '''add P-AI-1''' | ||
+ | |||
+ | pBAD→RBS+LasR+TT (from James DC) | ||
+ | |||
+ | I13453+B0034+C0079+B0015 | ||
+ | |||
+ | plasR/cI→RBS+GFP+tt | ||
+ | |||
+ | K09110?+E0240 glows | ||
+ | |||
+ | |||
+ | '''Ligation plan for test sequences''' | ||
+ | |||
+ | Digest plasR/cI with S/P, E0240 with X/P; do ligation. |
Latest revision as of 14:54, 11 July 2008
pLasR/cI is constitutively off and is repressed by cI. The addition of p-AI-1, activates LasR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.
LasR | cI | p-AI-1 + GFP | p-AI-1 - GFP |
---|---|---|---|
0 | 0 | 0 | 0 |
1 | 0 | 1 | 0 |
0 | 1 | 0 | 0 |
1 | 1 | 0 | 0 |
Test pLasR/cI:
pBAD→RBS+LasR+TT (from James DC)
I13453+B0034+C0079+B0015
plasR/cI→RBS+GFP+tt
K09110?+E0240 does not glow
add P-AI-1
pBAD→RBS+LasR+TT (from James DC)
I13453+B0034+C0079+B0015
plasR/cI→RBS+GFP+tt
K09110?+E0240 glows
Ligation plan for test sequences
Digest plasR/cI with S/P, E0240 with X/P; do ligation.