Difference between revisions of "Build XOR Gate"
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| − | + | [[LuxR/cI]] | |
| − | + | [[LsrA/cI]] | |
| − | |||
| − | + | [[LasR/cI]] | |
| − | + | [[Mnt/Lac]] | |
| − | |||
| − | |||
| + | [[Mnt/Tet]] | ||
| + | '''Combined Parts''' | ||
| + | pLux/cI+RBS+LuxS+RBS+Mnt+TT+pLac/Mnt+RBS+LuxS+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT | ||
| + | pLsr/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pBAD+RBS+LsrK+TT | ||
| − | |||
| + | pLux/cI+RBS+LasI+RBS+Mnt+TT+pLac/Mnt+RBS+LasI+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT | ||
| − | '''Mnt/Tet | + | pLas/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pLacI+RBS+LsrR+TT |
| + | |||
| + | |||
| + | These parts will be placed in a medium copy amp plasmid (probably pSB4A3) | ||
| + | |||
| + | |||
| + | '''Overlapping Parts And intermediates''' | ||
| + | |||
| + | |||
| + | {| border="1" cellpadding="2" | ||
| + | !width="30"|LuxR/cI | ||
| + | !width="30"|Lac/Mnt | ||
| + | !width="30"|LsrR/cI | ||
| + | !width="30"|LasR/cI | ||
| + | !width="30"|Mnt/Tet | ||
| + | |- | ||
| + | | || || C0261(RBS+LuxI) || C0261(RBS+LuxI) || C0261 (RBS+LuxI) | ||
| + | |- | ||
| + | | || S0103(RBS+cI) || || || S0103(RBS+cI) | ||
| + | |- | ||
| + | | I13025(RBS+mnt) || || I13025(RBS+Mnt) || I13025(RBS+Mnt) || | ||
| + | |- | ||
| + | | K091109(LuxS) || K091109(LuxS) || || || | ||
| + | |- | ||
| + | | RBS+Mnt+TT || || RBS+Mnt+TT || RBS+Mnt+TT || | ||
| + | |- | ||
| + | | || RBS+cI+TT || || || RBS+cI+TT | ||
| + | |- | ||
| + | | RBS+luxS || RBS+luxS || || || | ||
| + | |- | ||
| + | | RBS+lasI || RBS+lasI || || || | ||
| + | |- | ||
| + | | || || || || | ||
| + | |- | ||
| + | | || || || || | ||
| + | |- | ||
| + | | || || || || | ||
| + | |- | ||
| + | | || || || || | ||
| + | |} | ||
Latest revision as of 15:24, 6 August 2008
Combined Parts
pLux/cI+RBS+LuxS+RBS+Mnt+TT+pLac/Mnt+RBS+LuxS+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT
pLsr/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pBAD+RBS+LsrK+TT
pLux/cI+RBS+LasI+RBS+Mnt+TT+pLac/Mnt+RBS+LasI+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT
pLas/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pLacI+RBS+LsrR+TT
These parts will be placed in a medium copy amp plasmid (probably pSB4A3)
Overlapping Parts And intermediates
| LuxR/cI | Lac/Mnt | LsrR/cI | LasR/cI | Mnt/Tet |
|---|---|---|---|---|
| C0261(RBS+LuxI) | C0261(RBS+LuxI) | C0261 (RBS+LuxI) | ||
| S0103(RBS+cI) | S0103(RBS+cI) | |||
| I13025(RBS+mnt) | I13025(RBS+Mnt) | I13025(RBS+Mnt) | ||
| K091109(LuxS) | K091109(LuxS) | |||
| RBS+Mnt+TT | RBS+Mnt+TT | RBS+Mnt+TT | ||
| RBS+cI+TT | RBS+cI+TT | |||
| RBS+luxS | RBS+luxS | |||
| RBS+lasI | RBS+lasI | |||
