Difference between revisions of "Mnt/Tet"
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(17 intermediate revisions by the same user not shown) | |||
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+ | '''Robert Cool''' | ||
+ | |||
'''Parts Relevant to XOR Gate:''' | '''Parts Relevant to XOR Gate:''' | ||
Line 9: | Line 11: | ||
| K091105 || pMnt/Tet || MW '08 || RC | | K091105 || pMnt/Tet || MW '08 || RC | ||
|- | |- | ||
− | | B0034 || RBS || registry || | + | | B0034 || RBS(34) || registry || RC |
|- | |- | ||
| C0061 || Lux gene(+LVA) || registry || TBD | | C0061 || Lux gene(+LVA) || registry || TBD | ||
Line 17: | Line 19: | ||
| C0051 || cI gene || registry || TBD | | C0051 || cI gene || registry || TBD | ||
|- | |- | ||
− | | B0015 || TT || registry || | + | | B0015 || TT || registry || RC |
+ | |- | ||
+ | | S0101 || RBS(34)+TetR || MW '08 || RC | ||
+ | |- | ||
+ | | I13453 || pBAD || registry || RC | ||
|- | |- | ||
− | | | + | | pSB2K3 || kan resistance || registry || TBD |
|- | |- | ||
− | | | + | | S0103 || RBS(34)+cI || registry || RC |
+ | |- | ||
+ | | C0261 || RBS(34)+LuxI || registry || RC | ||
+ | |- | ||
+ | | P0455 || RBS+cI+TT || registry || RC | ||
|- | |- | ||
− | |||
|} | |} | ||
+ | '''Parts Needed for XOR Gate''' | ||
− | + | {| border="1" cellpadding="2" | |
+ | !width="30"|part no. | ||
+ | !width="30"|description | ||
+ | !width="30"|source | ||
+ | !width="30"|responsible | ||
+ | |- | ||
+ | | K091105 || pMnt/Tet || MW '08 || RC | ||
+ | |- | ||
+ | | B0034 || RBS(34) || registry || TBD | ||
+ | |- | ||
+ | | B0015 || TT || registry || RC | ||
+ | |- | ||
+ | | S0101 || RBS(34)+TetR || MW '08 || RC | ||
+ | |- | ||
+ | | I13453 || pBAD || registry || RC | ||
+ | |- | ||
+ | | S0103 || RBS(34)+cI || registry || RC | ||
+ | |- | ||
+ | | C0261 || RBS(34)+LuxI || registry || RC | ||
+ | |- | ||
+ | | P0455 || RBS+cI+TT || registry || RC | ||
+ | |- | ||
+ | | pSB4A3 ||low copy Amp plasmid || registry || RC | ||
+ | |} | ||
Line 46: | Line 79: | ||
!width="30"|product BBa | !width="30"|product BBa | ||
!width="30"| product size | !width="30"| product size | ||
+ | !width="30"| status | ||
|- | |- | ||
− | | 1 || | + | | 1 || K091105 || pMnt/Tet || suffix || SP || 2200 || C0261 || RBS+LuxI || suffix, prefix || XP || 661 || TBD || 2861 || miniprep verified (K091132) |
− | | | ||
− | | | ||
|- | |- | ||
− | | | + | | 2 || B0015 || TT || prefix || RX || 2278 || S0101 || RBS+cI || suffix, prefix || RS || 793 || TBD || 3081 || same as P0455 |
|- | |- | ||
− | | | + | | 3 || product from step 1 || pMnt/Tet+RBS+LuxI || suffix || SP || || product from step 2 || RBS+cI+TT || suffix prefix || XP || || TBD || || miniprep verified (K091144) |
− | |||
− | |||
|} | |} | ||
Line 74: | Line 104: | ||
!width="30"|insert size (bp) | !width="30"|insert size (bp) | ||
!width="30"|product BBa | !width="30"|product BBa | ||
− | !width="30"| product size | + | !width="30"|product size |
+ | !width="30"|status | ||
|- | |- | ||
− | | 1 || I13453 || pBAD || suffix || SP || 2287 || S0101 || RBS+TetR || suffix, prefix || XP || 703 || TBD || 2990 | + | | 1 || I13453 || pBAD || suffix || SP || 2287 || S0101 || RBS+TetR || suffix, prefix || XP || 703 || TBD || 2990 || miniprep verified (K091133) |
|- | |- | ||
− | | 2 || B0015 || TT || prefix || RX || 2278 || product from step 1 || pBAD+RBS+TetR || suffix, prefix || RS || 833 || TBD || 3111 | + | | 2 || B0015 || TT || prefix || RX || 2278 || product from step 1 || pBAD+RBS+TetR || suffix, prefix || RS || 833 || TBD || 3111 || miniprep verified (K091145) |
|- | |- | ||
− | | 3 || | + | | 3 || pSB4A3 || low copy amp resistance || prefix, suffix || RP || 4425 || product from step 2 || pBAD+RBS+TetR+TT || suffix, prefix || RP || 954 || TBD || 5379|| |
|} | |} |
Latest revision as of 16:37, 4 August 2008
Robert Cool
Parts Relevant to XOR Gate:
part no. | description | source | responsible |
---|---|---|---|
K091105 | pMnt/Tet | MW '08 | RC |
B0034 | RBS(34) | registry | RC |
C0061 | Lux gene(+LVA) | registry | TBD |
C0062 | Lux gene(-LVA) | registry | TBD |
C0051 | cI gene | registry | TBD |
B0015 | TT | registry | RC |
S0101 | RBS(34)+TetR | MW '08 | RC |
I13453 | pBAD | registry | RC |
pSB2K3 | kan resistance | registry | TBD |
S0103 | RBS(34)+cI | registry | RC |
C0261 | RBS(34)+LuxI | registry | RC |
P0455 | RBS+cI+TT | registry | RC |
Parts Needed for XOR Gate
part no. | description | source | responsible |
---|---|---|---|
K091105 | pMnt/Tet | MW '08 | RC |
B0034 | RBS(34) | registry | TBD |
B0015 | TT | registry | RC |
S0101 | RBS(34)+TetR | MW '08 | RC |
I13453 | pBAD | registry | RC |
S0103 | RBS(34)+cI | registry | RC |
C0261 | RBS(34)+LuxI | registry | RC |
P0455 | RBS+cI+TT | registry | RC |
pSB4A3 | low copy Amp plasmid | registry | RC |
Steps Needed for building XOR Gate: pSB1A3
step no. | vector BBa | vector description | vector suffix prefix | vector enzymes | vector size (bp) | insert BBa | insert description | insert suffix prefix | insert enzymes | insert size (bp) | product BBa | product size | status |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | K091105 | pMnt/Tet | suffix | SP | 2200 | C0261 | RBS+LuxI | suffix, prefix | XP | 661 | TBD | 2861 | miniprep verified (K091132) |
2 | B0015 | TT | prefix | RX | 2278 | S0101 | RBS+cI | suffix, prefix | RS | 793 | TBD | 3081 | same as P0455 |
3 | product from step 1 | pMnt/Tet+RBS+LuxI | suffix | SP | product from step 2 | RBS+cI+TT | suffix prefix | XP | TBD | miniprep verified (K091144) |
TetR protein needed for XOR Gate: pSB2K3
step no. | vector BBa | vector description | vector suffix prefix | vector enzymes | vector size (bp) | insert BBa | insert description | insert suffix prefix | insert enzymes | insert size (bp) | product BBa | product size | status |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | I13453 | pBAD | suffix | SP | 2287 | S0101 | RBS+TetR | suffix, prefix | XP | 703 | TBD | 2990 | miniprep verified (K091133) |
2 | B0015 | TT | prefix | RX | 2278 | product from step 1 | pBAD+RBS+TetR | suffix, prefix | RS | 833 | TBD | 3111 | miniprep verified (K091145) |
3 | pSB4A3 | low copy amp resistance | prefix, suffix | RP | 4425 | product from step 2 | pBAD+RBS+TetR+TT | suffix, prefix | RP | 954 | TBD | 5379 |