Difference between revisions of "Build XOR Gate"
From GcatWiki
| (9 intermediate revisions by 2 users not shown) | |||
| Line 16: | Line 16: | ||
[[Mnt/Tet]] | [[Mnt/Tet]] | ||
| + | |||
| + | '''Combined Parts''' | ||
| + | |||
| + | pLux/cI+RBS+LuxS+RBS+Mnt+TT+pLac/Mnt+RBS+LuxS+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT | ||
| + | |||
| + | |||
| + | pLsr/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pBAD+RBS+LsrK+TT | ||
| + | |||
| + | |||
| + | pLux/cI+RBS+LasI+RBS+Mnt+TT+pLac/Mnt+RBS+LasI+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT | ||
| + | |||
| + | |||
| + | pLas/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pLacI+RBS+LsrR+TT | ||
| + | |||
| + | |||
| + | These parts will be placed in a medium copy amp plasmid (probably pSB4A3) | ||
| + | |||
'''Overlapping Parts And intermediates''' | '''Overlapping Parts And intermediates''' | ||
| Line 27: | Line 44: | ||
!width="30"|Mnt/Tet | !width="30"|Mnt/Tet | ||
|- | |- | ||
| − | | | + | | || || C0261(RBS+LuxI) || C0261(RBS+LuxI) || C0261 (RBS+LuxI) |
| + | |- | ||
| + | | || S0103(RBS+cI) || || || S0103(RBS+cI) | ||
|- | |- | ||
| − | | | + | | I13025(RBS+mnt) || || I13025(RBS+Mnt) || I13025(RBS+Mnt) || |
|- | |- | ||
| − | | | + | | K091109(LuxS) || K091109(LuxS) || || || |
|- | |- | ||
| − | | | + | | RBS+Mnt+TT || || RBS+Mnt+TT || RBS+Mnt+TT || |
|- | |- | ||
| − | | | + | | || RBS+cI+TT || || || RBS+cI+TT |
|- | |- | ||
| − | | | + | | RBS+luxS || RBS+luxS || || || |
|- | |- | ||
| − | | | + | | RBS+lasI || RBS+lasI || || || |
|- | |- | ||
| − | | | + | | || || || || |
|- | |- | ||
| − | | | + | | || || || || |
|- | |- | ||
| − | | | + | | || || || || |
|- | |- | ||
| − | | | + | | || || || || |
|} | |} | ||
Latest revision as of 15:24, 6 August 2008
Combined Parts
pLux/cI+RBS+LuxS+RBS+Mnt+TT+pLac/Mnt+RBS+LuxS+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT
pLsr/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pBAD+RBS+LsrK+TT
pLux/cI+RBS+LasI+RBS+Mnt+TT+pLac/Mnt+RBS+LasI+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT
pLas/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pLacI+RBS+LsrR+TT
These parts will be placed in a medium copy amp plasmid (probably pSB4A3)
Overlapping Parts And intermediates
| LuxR/cI | Lac/Mnt | LsrR/cI | LasR/cI | Mnt/Tet |
|---|---|---|---|---|
| C0261(RBS+LuxI) | C0261(RBS+LuxI) | C0261 (RBS+LuxI) | ||
| S0103(RBS+cI) | S0103(RBS+cI) | |||
| I13025(RBS+mnt) | I13025(RBS+Mnt) | I13025(RBS+Mnt) | ||
| K091109(LuxS) | K091109(LuxS) | |||
| RBS+Mnt+TT | RBS+Mnt+TT | RBS+Mnt+TT | ||
| RBS+cI+TT | RBS+cI+TT | |||
| RBS+luxS | RBS+luxS | |||
| RBS+lasI | RBS+lasI | |||
