Difference between revisions of "Build XOR Gate"
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[[Mnt/Tet]] | [[Mnt/Tet]] | ||
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| + | '''Combined Parts''' | ||
| + | |||
| + | pLux/cI+RBS+LuxS+RBS+Mnt+TT+pLac/Mnt+RBS+LuxS+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT | ||
| + | |||
| + | |||
| + | pLsr/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pBAD+RBS+LsrK+TT | ||
| + | |||
| + | |||
| + | pLux/cI+RBS+LasI+RBS+Mnt+TT+pLac/Mnt+RBS+LasI+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT | ||
| + | |||
| + | |||
| + | pLas/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pLacI+RBS+LsrR+TT | ||
| + | |||
| + | |||
| + | These parts will be placed in a medium copy amp plasmid (probably pSB4A3) | ||
| + | |||
'''Overlapping Parts And intermediates''' | '''Overlapping Parts And intermediates''' | ||
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!width="30"|Mnt/Tet | !width="30"|Mnt/Tet | ||
|- | |- | ||
| − | | || || C0261 || || C0261 | + | | || || C0261(RBS+LuxI) || C0261(RBS+LuxI) || C0261 (RBS+LuxI) |
|- | |- | ||
| − | | || S0103 || || || S0103 | + | | || S0103(RBS+cI) || || || S0103(RBS+cI) |
|- | |- | ||
| − | | I13025 || || I13025 || | + | | I13025(RBS+mnt) || || I13025(RBS+Mnt) || I13025(RBS+Mnt) || |
|- | |- | ||
| − | | K091109 || K091109 || || || | + | | K091109(LuxS) || K091109(LuxS) || || || |
|- | |- | ||
| − | | RBS+Mnt+TT || || RBS+Mnt+TT || || | + | | RBS+Mnt+TT || || RBS+Mnt+TT || RBS+Mnt+TT || |
|- | |- | ||
| || RBS+cI+TT || || || RBS+cI+TT | | || RBS+cI+TT || || || RBS+cI+TT | ||
|- | |- | ||
| RBS+luxS || RBS+luxS || || || | | RBS+luxS || RBS+luxS || || || | ||
| + | |- | ||
| + | | RBS+lasI || RBS+lasI || || || | ||
|- | |- | ||
| || || || || | | || || || || | ||
Latest revision as of 15:24, 6 August 2008
Combined Parts
pLux/cI+RBS+LuxS+RBS+Mnt+TT+pLac/Mnt+RBS+LuxS+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT
pLsr/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pBAD+RBS+LsrK+TT
pLux/cI+RBS+LasI+RBS+Mnt+TT+pLac/Mnt+RBS+LasI+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT
pLas/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pLacI+RBS+LsrR+TT
These parts will be placed in a medium copy amp plasmid (probably pSB4A3)
Overlapping Parts And intermediates
| LuxR/cI | Lac/Mnt | LsrR/cI | LasR/cI | Mnt/Tet |
|---|---|---|---|---|
| C0261(RBS+LuxI) | C0261(RBS+LuxI) | C0261 (RBS+LuxI) | ||
| S0103(RBS+cI) | S0103(RBS+cI) | |||
| I13025(RBS+mnt) | I13025(RBS+Mnt) | I13025(RBS+Mnt) | ||
| K091109(LuxS) | K091109(LuxS) | |||
| RBS+Mnt+TT | RBS+Mnt+TT | RBS+Mnt+TT | ||
| RBS+cI+TT | RBS+cI+TT | |||
| RBS+luxS | RBS+luxS | |||
| RBS+lasI | RBS+lasI | |||
