Difference between revisions of "LsrA/cI"

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Line 1: Line 1:
 
'''Goal''':  
 
'''Goal''':  
  
−
'''pBAD+RBS+lsrK+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''
+
'''pBAD+RBS+lsrK+RBS+lsrR+TT'''
  
−
'''I13453'''+'''B0034'''+'''K091002'''+'''B0034'''+'''K091001'''+'''P0455'''(B0034+C0051+B0015)+'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''
+
'''pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''
 +
 
 +
'''I13453'''+'''B0034'''+'''K091002'''+'''B0034'''+'''K091001'''+'''B0015'''
 +
 
 +
'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''
  
  
Line 24: Line 28:
 
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''
 
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''
 
|-
 
|-
−
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12||K091001||lsrR  ||R/X;S/P  ||X/P  ||120 ||TBD ||
+
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091001||lsrR  ||R/X;S/P  ||X/P  ||954 ||K091138 ||972
 
|-
 
|-
−
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12||K091002||lsrK  ||R/X;S/P  ||X/P  ||516 ||TBD ||
+
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091002||lsrK  ||R/X;S/P  ||X/P  ||1593 ||K091158 ||1611
 
|-
 
|-
−
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120||TBD||RBS+lsrK ||R/X; S/P  ||X/P  || ||TBD ||  
+
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120+2079||TBD||RBS+lsrK ||R/X; S/P  ||X/P  ||1611 ||K091159 || 1866
 
|-
 
|-
−
| 4||TBD||pBAD+RBS+lsrK||R/X; S/P ||S/P || ||TBD ||RBS+lsrR  ||R/X; S/P  ||X/P || ||TBD ||
+
| 4||B0015||TT||R/X; S/P ||R/X ||129+2079 ||K091138 ||RBS+lsrR  ||R/X; S/P  ||R/S || 972||K091139 ||1109
 
|-
 
|-
−
| 5||P0455 ||RBS+cI+TT ||R/X; S/P ||R/X ||896 ||TBD ||pBAD+RBS+lsrK+RBS+lsrR ||R/X; S/P  ||R/S  || ||TBD ||
+
| 5||K091139||RBS+lsrR+TT||R/X; S/P ||R/X ||1109+2079 ||TBD ||pBAD+RBA+lsrK  ||R/X; S/P  ||R/S  ||1866 ||TBD ||
 
|-
 
|-
−
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141||C0261||RBS+luxI  ||R/X; S/P  ||X/P  ||661 ||TBD||
+
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI  ||R/X; S/P  ||X/P  ||661 ||K091140||810
 
|-
 
|-
−
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129 ||I13025 ||RBS+Mnt  ||R/X;S/P  ||R/S  ||331 ||TBD ||
+
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt  ||R/X;S/P  ||R/S  ||331 ||K091141 ||468
 
|-
 
|-
−
| 8||TBD ||RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD ||pLsrA/cI+RBS+luxI  ||R/X; S/P  ||R/S  || || TBD||
+
| 8||K091141||RBS+Mnt+TT ||R/X; S/P ||R/X || 468+2079||K091140 ||pLsrA/cI+RBS+luxI  ||R/X; S/P  ||R/S  ||810 || K091142||1286
−
|-
 
−
| 9|| TBD ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD||pBAD+RBS+lsrK+RBS+lsrR+RBS+cI+TT  ||R/X; S/P  ||R/S || ||TBD ||
 
 
|-
 
|-
 
|}
 
|}
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|-
 
|-
 
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07  ||Plt.1-24A ||pSB1A2 ||  
 
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07  ||Plt.1-24A ||pSB1A2 ||  
−
|-
 
−
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07  ||Plt.1-16M ||pSB1AC3 ||
 
−
|-
 
−
[http://partsregistry.org/Part:BBa_P0451 P0451]||RBS+cI+TT ||registry 07  ||Plt.1-21O ||pSB1AK3 ||
 
 
|-
 
|-
 
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07  ||Plt.1-23J ||pSB1A2 ||  
 
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07  ||Plt.1-23J ||pSB1A2 ||  

Latest revision as of 15:27, 28 July 2008

Goal:

pBAD+RBS+lsrK+RBS+lsrR+TT

pLsrA/cI+RBS+LuxI+RBS+Mnt+TT

I13453+B0034+K091002+B0034+K091001+B0015

K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015


Ligation Plan

Vector Insert Product
Step BBa_ Description Pre-/suffix Enzymes Size BBa_ Description Pre-/suffix Enzymes Size BBa_ Size
1 B0034 RBS R/X; S/P S/P 12+2079 K091001 lsrR R/X;S/P X/P 954 K091138 972
2 B0034 RBS R/X; S/P S/P 12+2079 K091002 lsrK R/X;S/P X/P 1593 K091158 1611
3 I13453 pBAD R/X;S/P S/P 120+2079 TBD RBS+lsrK R/X; S/P X/P 1611 K091159 1866
4 B0015 TT R/X; S/P R/X 129+2079 K091138 RBS+lsrR R/X; S/P R/S 972 K091139 1109
5 K091139 RBS+lsrR+TT R/X; S/P R/X 1109+2079 TBD pBAD+RBA+lsrK R/X; S/P R/S 1866 TBD
6 K091106 pLsrA/cI R/X; S/P S/P 141+2079 C0261 RBS+luxI R/X; S/P X/P 661 K091140 810
7 B0015 TT R/X; S/P R/X 129+2079 I13025 RBS+Mnt R/X;S/P R/S 331 K091141 468
8 K091141 RBS+Mnt+TT R/X; S/P R/X 468+2079 K091140 pLsrA/cI+RBS+luxI R/X; S/P R/S 810 K091142 1286

Parts

BBa_ Description Source location('07) Vector Responsible
B0015 double terminator registry 07 Plt.1-1I;Plt.3-3O pSB1AK3
B0034 RBS registry 07 Plt.1-3O pSB1A2
I13453 pBAD registry 07 Plt.2-13D; Plt.3-3I pSB1A3
C0261 RBS+luxI registry 07 Plt.1-24A pSB1A2
I13025 RBS+Mnt registry 07 Plt.1-23J pSB1A2
K091001 lsrR MW N/A N/A
K091002 lsrK MW N/A N/A
K091106 plsrA/cI MW N/A N/A
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