Difference between revisions of "Parts needed for XOR"
From GcatWiki
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|[http://partsregistry.org/Part:BBa_C0061 BBa_C0061]||. LuxI w/ LVA ||Plt.1-5O; pSB1A2 ||amp || || | |[http://partsregistry.org/Part:BBa_C0061 BBa_C0061]||. LuxI w/ LVA ||Plt.1-5O; pSB1A2 ||amp || || | ||
|- | |- | ||
| − | | | + | |[http://partsregistry.org/Part:pSB4A3 BBa_pSB4A3]|| low copy amp plasmid || || || || |
|- | |- | ||
|BBa_|| || || || || | |BBa_|| || || || || | ||
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|[http://partsregistry.org/Part:BBa_K091147 K091147] || pBAD+RBS+Mnt+TT || amp || right size || yes ||yes | |[http://partsregistry.org/Part:BBa_K091147 K091147] || pBAD+RBS+Mnt+TT || amp || right size || yes ||yes | ||
| + | |- | ||
| + | |[http://partsregistry.org/Part:BBa_K091160 K091160] || pMnt/Tet+RBS+GFP+TT || amp || right size || yes || yes | ||
| + | |- | ||
| + | | [http://partsregistry.org/Part:BBa_K091175 K091175] || K091145+K091160 || amp || right size || yes || yes | ||
| + | |- | ||
| + | | [http://partsregistry.org/Part:BBa_K091176 K091176] || K091147+K091160 || amp || right size || yes || yes | ||
| + | |- | ||
| + | | [http://partsregistry.org/Part:BBa_K091177 K091177] || K091160+K091145 || amp || right size || yes || yes | ||
| + | |- | ||
| + | | [http://partsregistry.org/Part:BBa_K091178 K091178] || K091160+K091147 || amp || right size || yes ||yes | ||
|- | |- | ||
|} | |} | ||
Latest revision as of 17:56, 6 August 2008
Basic Parts:
| Part | Description | Location(07 registry) and Vector | Resistance | Results | miniprep |
|---|---|---|---|---|---|
| BBa_C0051 | . cI w/ LVA | Plt.1-5G; pSB1A2 | amp | ||
| BBa_C0072 | . mnt w/ LVA | Plt.2-5M; pSB2K3 | kan | ||
| BBa_C0040 | . tetR w/ LVA | Plt.1-5C; pSB1A2 | amp | ||
| BBa_C0062 | . LuxR no LVA | Plt.1-7A; pSB1A2 | amp | ||
| BBa_C0061 | . LuxI w/ LVA | Plt.1-5O; pSB1A2 | amp | ||
| BBa_pSB4A3 | low copy amp plasmid | ||||
| BBa_ | |||||
| BBa_ | |||||
| BBa_ |
Composite Parts:
| Part | Description | Location(07 registry) and Vector | Resistance | Results | miniprep |
|---|---|---|---|---|---|
| BBa_J06550 | . RBS+cI | Plt.2-9B; pSB1A2 | amp | ||
| BBa_S0103 | . RBS+cI | Plt.1-15C; pSB1A2 | amp | ||
| BBa_I13025 | . RBS+mnt | Plt.1-23J; pSB1A2 | amp | ||
| BBa_S0101 | . RBS+tetR | Plt.1-13O; pSB1A2 | amp | ||
| BBa_J37033 | . RBS+LuxR(no LVA) | Plt.3-8F; pSB1A2 | amp | ||
| BBa_C0261 | . RBS+LuxI | Plt.1-24A; pSB1A2 | amp | ||
| BBa_ | |||||
| BBa_ | |||||
| BBa_ |
New Parts Constructed:
| Part | Description | Resistance | Results | miniprep | glycerol stock |
|---|---|---|---|---|---|
| BBa_K091107 | . Lux-cI hybrid promoter | right size | yes | ||
| BBa_K091100 | . pLac_lux hybrid promoter | ||||
| BBa_K091101 | . pTet_Lac hybrid promoter | ||||
| BBa_K091104 | . Mnt/LacI Hybrid Promoter | ||||
| BBa_K091105 | . Mnt/TetR Promoter | ||||
| BBa_K091106 | . LsrA/cI hybrid promoter | right size | yes | ||
| BBa_K091001 | . LsrR gene | ||||
| BBa_K091002 | . LsrK gene | ||||
| BBA_K091109 | . LuxS gene | ||||
| BBA_K091110 | . LacI promoter | ||||
| BBA_K091111 | . LacIQ promoter | ||||
| BBA_K091112 | . LacIQ1 promoter | ||||
| K091132 | pMnt/Tet+RBS+LuxI | amp | right size | yes | yes |
| K091133 | pBAD+RBS+TetR | amp | right size | yes | yes |
| K091137 | RBS+Mnt+TT | amp | right size | yes | yes |
| K091144 | pMnt/Tet+RBS+LuxI+RBS+cI+TT | amp | right size | yes | yes |
| K091145 | pBad+RBS+TetR+TT | amp | right size | yes | yes |
| K091147 | pBAD+RBS+Mnt+TT | amp | right size | yes | yes |
| K091160 | pMnt/Tet+RBS+GFP+TT | amp | right size | yes | yes |
| K091175 | K091145+K091160 | amp | right size | yes | yes |
| K091176 | K091147+K091160 | amp | right size | yes | yes |
| K091177 | K091160+K091145 | amp | right size | yes | yes |
| K091178 | K091160+K091147 | amp | right size | yes | yes |
