Difference between revisions of "Experience gained on new Registry parts"

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Bacterial fluorescence on plates
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'''Cell-to-cell signaling by XOR gate constructs''' - The ability of two different XOR gate constructs to send a signal via the Lux system was measured by coplating bacterial XOR clones with bacteria containing a Lux receiver construct.
  
description of experiment
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Positive control using a Lux sender and a Lux receiver (K09100).  Green fluorescence indicates LuxI expression in the Lux sender and receipt of the intercellular signal by the receiver.
  
sender / receiver control
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[[Image:9100_Sender.JPG|389px]]
  
negative control
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<br>
  
K73 / receiver
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XOR gate construct K091173 coplated with the K09100 Lux receiver.  Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.
  
K82 / receiver
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[[Image:9100_K82.JPG|389px]]
  
Others
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<br>
  
 +
XOR gate construct K091182 coplated with the K09100 Lux receiver.  Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.
  
This will contain the story and data for the measurement of the new hybrid promoters.
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[[Image:9100_K73.JPG|389px]]
  
http://gcat.davidson.edu/GcatWiki/images/0/04/K82K9100.jpg
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<br>
 
 
pLac improvement data
 
 
 
table
 
 
 
chart
 

Latest revision as of 01:00, 28 October 2008

Cell-to-cell signaling by XOR gate constructs - The ability of two different XOR gate constructs to send a signal via the Lux system was measured by coplating bacterial XOR clones with bacteria containing a Lux receiver construct.

Positive control using a Lux sender and a Lux receiver (K09100). Green fluorescence indicates LuxI expression in the Lux sender and receipt of the intercellular signal by the receiver.

9100 Sender.JPG


XOR gate construct K091173 coplated with the K09100 Lux receiver. Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.

9100 K82.JPG


XOR gate construct K091182 coplated with the K09100 Lux receiver. Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.

9100 K73.JPG