Difference between revisions of "Cellulase"

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Blue: Pathway <br>
 
Blue: Pathway <br>
 
Purple: Molecule or Protein <br>
 
Purple: Molecule or Protein <br>
[[Image:ECBLASTer.png]] <br>
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Enzyme 3.2.1.4 is the cellulase <br>
According to the EC BLASTer, our organism does not contain enzymes 2.7.7.34 or 2.4.1.29. I think it would be interesting to either directly test for these two enzymes, or give the organism Alpha-D-Glucose 1P and see if it can produce Beta-D-Glucose. If the organism doesn’t have these two enzymes, that would also be very interesting, because it would suggest that it has a different way of forming glucose. I also think it would be interesting to directly test for enzymes 2.7.7.9 and 2.4.1.12, because all three databases did not call these enzymes, but it looks like there could be sequences associated with these enzymes in our organism’s genome.
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Enzyme 3.2.1.19 is the cellobiohydrolase <br>
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Enzyme 3.2.1.12 is the beta-glucosidase <br>
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I think it would be interesting to directly test for the enzymes highlighted in red and allow the organism to grow in the absence of glucose. I think that performing these two experiments will give us a lot of insight into how this organism produces glucose, because if we see that the organism can grow in the absence of the glucose and that it doesn't have some of these enzymes highlighted in red, this would suggest that this organism might have a different pathway for breaking down cellulose.

Latest revision as of 15:56, 13 November 2008

Cellulase.png
Legend:
Green: H. utahensis has the enzyme
Red: "H. utahensis" does not have the enzyme
Blue: Pathway
Purple: Molecule or Protein
Enzyme 3.2.1.4 is the cellulase
Enzyme 3.2.1.19 is the cellobiohydrolase
Enzyme 3.2.1.12 is the beta-glucosidase
I think it would be interesting to directly test for the enzymes highlighted in red and allow the organism to grow in the absence of glucose. I think that performing these two experiments will give us a lot of insight into how this organism produces glucose, because if we see that the organism can grow in the absence of the glucose and that it doesn't have some of these enzymes highlighted in red, this would suggest that this organism might have a different pathway for breaking down cellulose.