Difference between revisions of "Sequencing at MWG Operon"
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===How to Activate SimpleSeq Samples and Order DNA Sequencing=== | ===How to Activate SimpleSeq Samples and Order DNA Sequencing=== | ||
| − | # Go to www.operon.com and log in. | + | # Go to [http://www.operon.com www.operon.com] and log in. Username is Dr. C's full email address and the password is the nickname for the algae used in Bio111. |
# In the “Order Now” drop-down menu on the right-hand side, click “Order – DNA sequencing”. | # In the “Order Now” drop-down menu on the right-hand side, click “Order – DNA sequencing”. | ||
# If you already have your SimpleSeq tubes, click “Activate SimpleSeq Reactions”. | # If you already have your SimpleSeq tubes, click “Activate SimpleSeq Reactions”. | ||
| Line 25: | Line 25: | ||
a. Mix 8 μL of template and 4 μL of primer for a total volume of 12 μL | a. Mix 8 μL of template and 4 μL of primer for a total volume of 12 μL | ||
b. Make template and primer solutions that match the concentration ranges listed below: | b. Make template and primer solutions that match the concentration ranges listed below: | ||
| + | [[Image:SimpleSeq.png]] | ||
| + | <br> | ||
Revision as of 01:57, 30 April 2010
How to Activate SimpleSeq Samples and Order DNA Sequencing
- Go to www.operon.com and log in. Username is Dr. C's full email address and the password is the nickname for the algae used in Bio111.
- In the “Order Now” drop-down menu on the right-hand side, click “Order – DNA sequencing”.
- If you already have your SimpleSeq tubes, click “Activate SimpleSeq Reactions”.
- Enter either the barcode located on the side of the tube rack or a barcode from any tube in the rack. Click “Activate”.
- Close out of that window.
- Select your appropriate “Format” and “Sample Format”
- In this lab, the “Format” is usually “Tubes” and the “Sample Format” is usually “Premix DNA Template and Primer”***
- For each DNA sample you’re submitting, enter:
- “Barcode”: the barcode number of the tube.
- “Sample Name”: the name of your sample.
- “Template Type”: select “Plasmid” if using miniprepped DNA
- “Size (bp)”: the size of the whole vector including the insert
- When you’re finished, click “Submit Samples”.
- NOTE: The system will automatically detect that the barcode belongs to a SimpleSeq tube and will enter a no charge order into the system ***
- Review your order and click “Proceed to Shopping Cart”.
- If you are satisfied with your shopping cart, click “Checkout”.
- Select your shipping address and billing address and click “Next”.
- Enter your payment information and click “Next”.
- In this lab, we pay with our PO number. Retrieve the PO number from Dr. Campbell.***
How to Submit SimpleSeq Samples
If your sample format is “Premix DNA Template and Primer”:
a. Mix 8 μL of template and 4 μL of primer for a total volume of 12 μL
b. Make template and primer solutions that match the concentration ranges listed below:
