Difference between revisions of "Summer 2013 SynBio Project (Davidson and MWSU)"
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High Priority = more people  | High Priority = more people  | ||
| − | + | * eCDM8 working to make theophylline with biosensor and fitness modules (Tet^R) to report out. <br>  | |
Which plasmid carries which device?<br>  | Which plasmid carries which device?<br>  | ||
| − | + | * Determine the junction sequences and associated primers<br>  | |
(use existing rules)<br>  | (use existing rules)<br>  | ||
build scaffold <br>  | build scaffold <br>  | ||
test JGG design<br>  | test JGG design<br>  | ||
| − | + | * Stress module needs more work (see eCDM8 outputs)<br>  | |
| − | + | * test fitness module with adhE  | |
compare with tetA<br>  | compare with tetA<br>  | ||
| + | fine tune tetA resistance<br>  | ||
combine with tetA<br>  | combine with tetA<br>  | ||
| − | + | * Copy number of plasmids <br>  | |
| + | Can we alter Ori to produce a range of plasmid densities in ''E. coli''? <br>  | ||
Lower Priority = one person  | Lower Priority = one person  | ||
Revision as of 14:01, 30 May 2013
Google Hangout 11 am Wednesday (10 Central)
Topics to Investigate
High Priority = more people
-  eCDM8 working to make theophylline with biosensor and fitness modules (Tet^R) to report out. 
 
Which plasmid carries which device?
-  Determine the junction sequences and associated primers
 
(use existing rules)
build scaffold 
test JGG design
-  Stress module needs more work (see eCDM8 outputs)
 - test fitness module with adhE
 
compare with tetA
fine tune tetA resistance
combine with tetA
-  Copy number of plasmids 
 
Can we alter Ori to produce a range of plasmid densities in E. coli? 
Lower Priority = one person