Difference between revisions of "Davidson Missouri W/Davidson Protocols"
From GcatWiki
MaCampbell (talk | contribs) |
MaCampbell (talk | contribs) |
||
| Line 2: | Line 2: | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents] | ||
# [http://parts.mit.edu/registry/index.php/Assembly:Standard_assembly Standard Assembly] | # [http://parts.mit.edu/registry/index.php/Assembly:Standard_assembly Standard Assembly] | ||
| + | # [http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix BioBrick Ends] | ||
#[http://www.bio.davidson.edu/courses/Molbio/Protocols/ORIs.html '''Compatibility of Plasmids'''] | #[http://www.bio.davidson.edu/courses/Molbio/Protocols/ORIs.html '''Compatibility of Plasmids'''] | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/anneal_oligos.html Building dsDNA with Oligos] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/anneal_oligos.html Building dsDNA with Oligos] | ||
Revision as of 15:01, 27 May 2008
- How to Keep a Lab Notebook
- Common molecular reagents
- Standard Assembly
- BioBrick Ends
- Compatibility of Plasmids
- Building dsDNA with Oligos
- Setting up PCR mixtures
- PCR and Mg2+ concentration
- Clean and Concentrate DNA (after PCR, before digestion)
- Pouring an agarose gel
- Calculate MWs
- Digest DNA with restriction enzymes
- Double Digest Guide
- Ethanol Precipitate DNA (short protocol)
- 1kb MW markers
- Shrimp Alkaline Phosphatase
- Qiagen QIAquick Gel Purification
- Qiagen QIAquick Column Regeneration Protocol
- ElectroElute Gel Purification
- Ligation Protocol
- Heat Shock Transformation OR Short version of Heat Shock
- Zippy Transformation
- Colony PCR to Screen for Successful Ligations
- Promega miniprep
- Choices for Transformation: Heat Shock vs. Zyppy
- Choices for Mini-Preps: Promega vs. Zyppy
- Sequencing at CUGI
- Making dsDNA Using Primer Dimers
