Difference between revisions of "Davidson Missouri W/Davidson Protocols"

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# [[Davidson Missouri W/CUGI_Seuqencing| Sequencing at CUGI]]
 
# [[Davidson Missouri W/CUGI_Seuqencing| Sequencing at CUGI]]
 
# [[Davidson Missouri W/Primer_dimer| Making dsDNA Using Primer Dimers]]
 
# [[Davidson Missouri W/Primer_dimer| Making dsDNA Using Primer Dimers]]
 +
#[http://gcat.davidson.edu/iGEM07/genesplitter.html Gene Splitting Web Site]

Revision as of 14:12, 2 June 2008

  1. How to Keep a Lab Notebook
  2. Common molecular reagents
  3. Standard Assembly
  4. BioBrick Ends
  5. Compatibility of Plasmids
  6. Building dsDNA with Oligos
  7. Setting up PCR mixtures
  8. PCR and Mg2+ concentration
  9. Clean and Concentrate DNA (after PCR, before digestion)
  10. Pouring an agarose gel
  11. Calculate MWs
  12. Digest DNA with restriction enzymes
  13. Double Digest Guide
  14. Ethanol Precipitate DNA (short protocol)
  15. 1kb MW markers
  16. Shrimp Alkaline Phosphatase
  17. Qiagen QIAquick Gel Purification
  18. Qiagen QIAquick Column Regeneration Protocol
  19. ElectroElute Gel Purification
  20. Ligation Protocol
  21. Heat Shock Transformation OR Short version of Heat Shock
  22. Zippy Transformation
  23. Colony PCR to Screen for Successful Ligations
  24. Promega miniprep
  25. Choices for Transformation: Heat Shock vs. Zyppy
  26. Choices for Mini-Preps: Promega vs. Zyppy
  27. Sequencing at CUGI
  28. Making dsDNA Using Primer Dimers
  29. Gene Splitting Web Site
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