Difference between revisions of "Divide and Conquer Biological Challenges"
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MaCampbell (talk | contribs) (→* We need to have an XOR logic gate produced.) |
MaCampbell (talk | contribs) (→* We need to have an XOR logic gate produced.) |
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=== * We need to have an XOR logic gate produced. === | === * We need to have an XOR logic gate produced. === | ||
'''Existing Logic Gates''' | '''Existing Logic Gates''' | ||
| − | *NOT:<br> | + | *'''NOT''':<br> |
# [http://partsregistry.org/Part:BBa_I732205 I732205 (NOT/ Dual-repressed NOR)] | # [http://partsregistry.org/Part:BBa_I732205 I732205 (NOT/ Dual-repressed NOR)] | ||
| − | *AND: <br> | + | <br> |
| − | '''Existing But Complex''' | + | *'''AND''': <br> |
| + | [http://www.bio.davidson.edu/Courses/Synthetic/papers/ANDgate.pdf '''Existing But Complex'''] | ||
[[Image:AND_Gate.png]]<br> | [[Image:AND_Gate.png]]<br> | ||
<br> | <br> | ||
'''Simple But Hypothetical''' | '''Simple But Hypothetical''' | ||
[[Image:Tet_AND_Lac.jpg]] | [[Image:Tet_AND_Lac.jpg]] | ||
| − | [[Image:AND_Table.jpg]] | + | [[Image:AND_Table.jpg]]<br> |
| − | + | We would need to have: <br> | |
| − | *NAND:<br> | + | # [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 Starts with pTetR] |
| + | # over production of LacI<sup>Q</sup> repressor | ||
| + | # over production of TetR repressor<br> | ||
| + | <br> | ||
| + | *'''NAND''':<br> | ||
# [http://partsregistry.org/Part:BBa_I732914 I732914] | # [http://partsregistry.org/Part:BBa_I732914 I732914] | ||
| − | *NOR:<br> | + | <br> |
| + | *'''NOR''':<br> | ||
# [http://partsregistry.org/Part:BBa_I732916 I732916]<br> | # [http://partsregistry.org/Part:BBa_I732916 I732916]<br> | ||
# [http://partsregistry.org/Part:BBa_I732917 I732917]\<br> | # [http://partsregistry.org/Part:BBa_I732917 I732917]\<br> | ||
| − | *Inverters:<br> | + | <br> |
| + | *'''Inverters''':<br> | ||
''Self-Contained Inverters'' | ''Self-Contained Inverters'' | ||
# [http://partsregistry.org/Part:BBa_J23040 J23040 (AHL-dependent inverter)]<br> | # [http://partsregistry.org/Part:BBa_J23040 J23040 (AHL-dependent inverter)]<br> | ||
Revision as of 15:16, 29 May 2008
Contents
- 1 * We need to get the Lux/AHL system working.
- 2 * We need to get the Lsr/AI-2 system working.
- 3 * We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
- 4 * We need to have an XOR logic gate produced.
- 5 * We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
* We need to get the Lux/AHL system working.
* We need to get the Lsr/AI-2 system working.
Our summary of the Lsr system: Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system
- There are NO Lsr parts in the registry:
- We will need to make BB parts for:
- LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp.
- Lsr promoter
- LuxS that produces DPD that somehow is converted to R-THMF.
- How do we get cells to make AI-2?
* We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
* We need to have an XOR logic gate produced.
Existing Logic Gates
- NOT:
- AND:
Existing But Complex
Simple But Hypothetical
We would need to have:
- Starts with pTetR
- over production of LacIQ repressor
- over production of TetR repressor
- NAND:
- NOR:
- Inverters:
Self-Contained Inverters
PoPS Output Inverters
* We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
- What if AmpR is secreted and cells are not AmpR? This would prevent cells down the chain from responding too soon.
