Difference between revisions of "Test Mnt/Tet promoter"

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Test tet repression
+
'''1) Test tet repression:'''
  
 
Some promoter (pbad)→RBS+TetR+t+t+pMnt/Tet→RBS+GFP does not glows
 
Some promoter (pbad)→RBS+TetR+t+t+pMnt/Tet→RBS+GFP does not glows
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Test Mnt repression:
+
'''2) Test Mnt repression:'''
  
 
Some promoter→Mnt+t+t+pMnt/Tet→GFP glows
 
Some promoter→Mnt+t+t+pMnt/Tet→GFP glows
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Test Tet and Mnt repression:
+
'''3) Test Tet and Mnt repression:'''
  
 
Some promoter→TetR+Mnt+t+t+pMnt/Tet→GFP does not glows
 
Some promoter→TetR+Mnt+t+t+pMnt/Tet→GFP does not glows
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'''06/30/08'''
 
'''06/30/08'''
 +
 
K091105 ligated with E0240
 
K091105 ligated with E0240
  
need to ligate some promoter with an RBS and ligate TetR with a double terminator
+
need to ligate some promoter (digest with E and S) with an RBS (digest E and X) and ligate TetR(digest with E and S) with a double terminator (digest with E and x)
  
 
then ligate those two parts together
 
then ligate those two parts together
  
 
then ligate that part with the Mnt/Tet with GFP
 
then ligate that part with the Mnt/Tet with GFP

Revision as of 19:26, 30 June 2008

Test Mnt/Tet promoter

The pMnt/TetR promoter is constitutively on and is repressed by the TetR protein. The addition of aTc should repress the TetR causing it to glow. The addition of the Mnt protein should repress the promoter causing it not glow.


1) Test tet repression:

Some promoter (pbad)→RBS+TetR+t+t+pMnt/Tet→RBS+GFP does not glows

I13453+RBS+C0040+TT+K091105+E0240

add aTc

Some promoter→TetR+t+t+pMnt/Tet→GFP glows

I13453+RBS+C0040+TT+K091105+E0240


2) Test Mnt repression:

Some promoter→Mnt+t+t+pMnt/Tet→GFP glows

I13453+RBS+C0072+TT+K091105+E0240


3) Test Tet and Mnt repression:

Some promoter→TetR+Mnt+t+t+pMnt/Tet→GFP does not glows

I13453+RBS+C0040+C0072+TT+K091105+E0240

add aTc does not glow


06/30/08

K091105 ligated with E0240

need to ligate some promoter (digest with E and S) with an RBS (digest E and X) and ligate TetR(digest with E and S) with a double terminator (digest with E and x)

then ligate those two parts together

then ligate that part with the Mnt/Tet with GFP