Difference between revisions of "LasR/cI"

Revision as of 18:21, 22 July 2008

Goal:

pBAD+RBS+lasR+TT(James DC)

S0388?(I13453+B0034+C0079+B0015)

pLasR/cI+RBS+LuxI+RBS+Mnt+TT

K091143+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015

Ligation Plan

Parts

BBa_	Description	Source	location('07)	Vector
B0015	double terminator	registry 07	Plt.1-1I;Plt.3-3O	pSB1AK3
I13025	RBS+Mnt	registry 07	Plt.1-23J	pSB1A2
K091143	plasR/cI	MW	N/A	pSB1A2
C0261	RBS+luxI	registry 07	Plt.1-24A	pSB1A2

@@ Line 27: / Line 27: @@
 |'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''
 |-
-| 1|| K091141||RBS+Mnt+TT  ||R/X;S/P  ||R/X ||468+2079 ||C0261||RBS+luxI  ||R/X;S/P  ||R/X  ||661 ||TBD ||
+| 1|| K091141||RBS+Mnt+TT  ||R/X;S/P  ||R/X ||468+2079 ||C0261||RBS+luxI  ||R/X;S/P  ||R/X  ||661 ||K091154 ||1137
 |-
-| 2||K091143||plasR/cI ||R/X;S/P ||S/P ||164+2079 ||TBD||RBS+luxI+RBS+Mnt+TT ||R/X; S/P  ||X/P  || ||TBD ||
+| 2||K091143||plasR/cI ||R/X;S/P ||S/P ||164+2079 ||K091154||RBS+luxI+RBS+Mnt+TT ||R/X; S/P  ||X/P  ||1137 ||TBD ||
 |-
 |}