Difference between revisions of "Describe cellular communication systems used"

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LuxI sender
 
LuxI sender
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== Lsr (AI-2) cell signaling system ==
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'''Responsible''': Kelly Davis, Xiao Zhu
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'''Signal molecule''': AI-2 (furanosyl borate diester in V. harveyi, a variety of other molecules in other species), all are derived from DPD. [http://iai.asm.org/cgi/reprint/73/6/3197.pdf AI-2 is R-THMF in E. coli]
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[http://www.biomedcentral.com/content/pdf/1471-2148-4-36.pdf]
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'''Bacterial species''':
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lsrA,B,C,D,F,G,R,K: Escherichia coli HS, SMS-3-5, str. K12 substr. MG1655, and substr. DH10B.
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lsrE:Escherichia coli str. K12 substr. MG1655
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LuxS:Escherichia coli HS, SMS-3-5, APEC O1, str. K12 substr. MG1655, substr. DH10B, and UTI89.
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'''Receiver protein''': LsrR protein receives signal from sensor protein
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'''Signal molecule synthase''': Pfs enzyme, then LuxS autoinducer synthase
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'''Target genes''': lsr operon, including ABC transporter and LsrK kinase
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'''Regulation''': LsrR represses the lsr operon, derepression by phospho-AI-2; catabolite repression influences AI-2 accumulation through the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex, which directly stimulates transcription of the lsr operon and indirectly represses luxS expression.cAMP-CRP is shown to bind to a cAMP receptor protein (CRP) binding site located in the upstream region of the lsr promoter and works with the LsrR repressor to regulate AI-2 uptake.
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'''Note:''' AI-2 is synthesized and secreted during exponential growth and is imported in stationary phase when glucose becomes limiting. In the presence of glucose, AI-2 is not imported because the lsr operon is not transcribed due to camp-CAP mediated repression. Both glycerol and G3P(glycerol 3-phosphate) repress lsr transcription, while the majority repression comes from G3P. DHAP represses lsr transcription by a cAMP-CAP-independent mechanism involving LsrR.
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'''Parts Needed:'''
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LsrR pro/term
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LsrK
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LsrACDB (transport)
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LsrFGE (catabolic)
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LuxS
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Pfs enzyme (?)
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http://gcat.davidson.edu/GcatWiki/images/b/b8/N654260305_1291548_2335.jpg
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'''Note:'''
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lsrB encodes the periplasmic AI-2 binding protein
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lsrC & lsrD encode the channel proteins
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lsrA encodes the ATPase that provides energy for AI-2 transport
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lsrF is similar to genes specifying aldolases
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lsrG encodes a protein with an unknown function.
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[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5591389&ordinalpos=26&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum tam: trans-aconitate 2-methyltransferase, also known as lsrE or yneD]  [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6061192&ordinalpos=10&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum yneE:conserved inner membrane protein]
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http://gcat.davidson.edu/GcatWiki/images/3/30/N654260305_1340817_550.jpg
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[[Image:Har.JPG]]
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=55669965 R-THMF]
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http://gcat.davidson.edu/GcatWiki/images/8/8c/S-DPD.gif
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http://www.nature.com/nrmicro/journal/v3/n5/images/nrmicro1146-f2.gif
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http://gcat.davidson.edu/GcatWiki/images/c/c9/Grl.jpg
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DHAP: dihydroxyacetone phosphate.
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http://gcat.davidson.edu/GcatWiki/images/9/9b/Grlw.jpg
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'''References'''
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[http://jb.asm.org/cgi/content/full/187/6/2066?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&titleabstract=lsrR&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT Cyclic AMP and cAMP Receptor Protein Influence both Synthesis and Uptake of Extracellular Autoinducer 2 in Escherichia coli]
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[http://iai.asm.org/cgi/reprint/IAI.00550-07v1.pdf Global Effects of the Cell-to-Cell Signaling Molecules Autoinducer-2, Autoinducer-3, and Epinephrine in a luxS Mutant of Enterohemorrhagic Escherichia Coli]
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[http://www.rsc.org/delivery/_ArticleLinking/DisplayHTMLArticleforfree.cfm?JournalCode=CC&Year=2005&ManuscriptID=b509396a&Iss=38 Shows how AI-2 is formed]
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[http://www.jstor.org/sici?sici=0027-8424(20031125)100%3C14549%3ACCAB%3E2.0.CO%3B2-B&cookieSet=1 Signaling explained with graphics of AI-2 pathways]
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[http://web.ebscohost.com/ehost/detail?vid=1&hid=116&sid=edfbf2f7-b0c8-40c3-8227-1cc94f134972%40sessionmgr108 Lsr-mediated transport and processing of AI-2 in Salmonella typhimurium]
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[http://www.microbialcellfactories.com/content/pdf/1475-2859-1-5.pdf Review of AI-2 and other systems]
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[http://jb.asm.org/cgi/content/full/189/16/6011?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&titleabstract=AI+2+LsrR&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT Quorum Sensing in Escherichia coli Is Signaled by AI-2/LsrR: Effects on Small RNA and Biofilm Architecture]
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[http://jb.asm.org/cgi/content/full/187/1/238?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&titleabstract=quorum+sensing+AI-2&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli]
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'''Resources'''
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[http://www.ommscientific.com/ AI-2/DPD]
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[http://www.biomedcentral.com/content/pdf/1471-2180-8-98.pdf alternate AI-2 synthesis]
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[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=full_report&list_uids=5586283 lsrK gene in Entrez]
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[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6062136&ordinalpos=9&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum#summary lsrR gene in Entrez]
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[http://BioCyc.org/ECOLI/substring-search?type=NIL&object=lsr lsr nucleotide sequence in EcoCyc]
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Transcription of LuxS (interaction with micA,gshA)
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http://gcat.davidson.edu/GcatWiki/images/c/cb/N654260305_1347432_6212.jpg

Revision as of 14:51, 28 October 2008

Las/Rhl cell signaling system

Responsible: Robert Cool, Alicia Allen, and Erin Feeney

Las System

Signal Molecule: An AHL called PAI-1 (N-3-oxododecanoyl-l-hsl)(3-oxo-C12-hsl)

Bacterial species: Pseudomonas aeruginosa gram(-) possibly E.coli (see article 3)

Receiver protein: LasR

Effect of binding: TXN activation of virulence genes, lasA, lasB, apr, toxR

Synthase: LasI enzyme

Target Genes: lasI, lasA, lasB, apr, toxR

Regulation: unknown


References

"Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes"

JP Pearson, EC Pesci and BH Iglewski [1]


"Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa"

Dana S. Wade, M. Worth Calfee, Edson R. Rocha, Elizabeth A. Ling, Elana Engstrom, James P. Coleman, and Everett C. Pesci 2


Posttranscriptional Control of Quorum-Sensing-Dependent Virulence Genes by DksA in Pseudomonas aeruginosa

Florence Jude,Thilo Köhler,Pavel Branny,Karl Perron,Matthias P. Mayer,Rachel Comte, and Christian van Delden 3

Pending: [2]

Rhl System


Signal Molecule: An AHL called PAI-2, Plasminogen activator inhibitor-2, N-butanoyl-homoserine lactone (C4HSL)

Bacterial species: Pseudomonas aeruginosa, gram(-)

Receiver Protein: Rhl R

Effect of Binding: activation of Rhamnosyl Transferase, then making RL (rhamnolipid)

Synthase: RhlA and RhlB

Target Genes: pqsABCDE and phnAB

Regulation: unknown

References

background information on Las and Rhl

Las rhl.gif

Parts Needed:

LasR + pro/term

RhlR + pro/term

RhlI + pro/term

pqsABCDE + pro/term

pqsR

pqsH + pro/term

phnAB

LasI

RBS

Lux cell signaling system

Responsible: Andrew Gordon and Pallavi Penumetcha

Signal molecule: N-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules

Bacterial species: discovered in Vibrio fischeri known to work in E. coli

Receiver protein: LuxR protein receives signal from AHL; also has some control over transciption of luciferase

Signal molecule synthase: LuxI; also has some control over transciption of luciferase

Additional Information: "Quorum Quenching" aiiA (intracellular) lactonase reduces AHL concentration

800px-Luxrreceiverschematic.png

Resources

Lux Operon Pathway

AHL signaling molecules by species; some are specific to gram pos but may affect gram negs

Reducing Crosstalk in Lux System

References

Quorum Quenching to control Lux Pathway

[3] A Synthetic multicellular system for programmed pattern formation

Parts Needed:

LuxR + pro/term

RBS

LuxI + pro/term

LuxI sender

Lsr (AI-2) cell signaling system

Responsible: Kelly Davis, Xiao Zhu

Signal molecule: AI-2 (furanosyl borate diester in V. harveyi, a variety of other molecules in other species), all are derived from DPD. AI-2 is R-THMF in E. coli

[4]

Bacterial species:

lsrA,B,C,D,F,G,R,K: Escherichia coli HS, SMS-3-5, str. K12 substr. MG1655, and substr. DH10B.

lsrE:Escherichia coli str. K12 substr. MG1655

LuxS:Escherichia coli HS, SMS-3-5, APEC O1, str. K12 substr. MG1655, substr. DH10B, and UTI89.

Receiver protein: LsrR protein receives signal from sensor protein

Signal molecule synthase: Pfs enzyme, then LuxS autoinducer synthase

Target genes: lsr operon, including ABC transporter and LsrK kinase

Regulation: LsrR represses the lsr operon, derepression by phospho-AI-2; catabolite repression influences AI-2 accumulation through the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex, which directly stimulates transcription of the lsr operon and indirectly represses luxS expression.cAMP-CRP is shown to bind to a cAMP receptor protein (CRP) binding site located in the upstream region of the lsr promoter and works with the LsrR repressor to regulate AI-2 uptake.

Note: AI-2 is synthesized and secreted during exponential growth and is imported in stationary phase when glucose becomes limiting. In the presence of glucose, AI-2 is not imported because the lsr operon is not transcribed due to camp-CAP mediated repression. Both glycerol and G3P(glycerol 3-phosphate) repress lsr transcription, while the majority repression comes from G3P. DHAP represses lsr transcription by a cAMP-CAP-independent mechanism involving LsrR.

Parts Needed:

LsrR pro/term

LsrK

LsrACDB (transport)

LsrFGE (catabolic)

LuxS

Pfs enzyme (?)

http://gcat.davidson.edu/GcatWiki/images/b/b8/N654260305_1291548_2335.jpg

Note:

lsrB encodes the periplasmic AI-2 binding protein

lsrC & lsrD encode the channel proteins

lsrA encodes the ATPase that provides energy for AI-2 transport

lsrF is similar to genes specifying aldolases

lsrG encodes a protein with an unknown function.

tam: trans-aconitate 2-methyltransferase, also known as lsrE or yneD yneE:conserved inner membrane protein

http://gcat.davidson.edu/GcatWiki/images/3/30/N654260305_1340817_550.jpg

Har.JPG R-THMF http://gcat.davidson.edu/GcatWiki/images/8/8c/S-DPD.gif http://www.nature.com/nrmicro/journal/v3/n5/images/nrmicro1146-f2.gif

http://gcat.davidson.edu/GcatWiki/images/c/c9/Grl.jpg

DHAP: dihydroxyacetone phosphate.

http://gcat.davidson.edu/GcatWiki/images/9/9b/Grlw.jpg

References

Cyclic AMP and cAMP Receptor Protein Influence both Synthesis and Uptake of Extracellular Autoinducer 2 in Escherichia coli

Global Effects of the Cell-to-Cell Signaling Molecules Autoinducer-2, Autoinducer-3, and Epinephrine in a luxS Mutant of Enterohemorrhagic Escherichia Coli

Shows how AI-2 is formed

Signaling explained with graphics of AI-2 pathways

Lsr-mediated transport and processing of AI-2 in Salmonella typhimurium

Review of AI-2 and other systems

Quorum Sensing in Escherichia coli Is Signaled by AI-2/LsrR: Effects on Small RNA and Biofilm Architecture

Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli

Resources

AI-2/DPD

alternate AI-2 synthesis

lsrK gene in Entrez

lsrR gene in Entrez

lsr nucleotide sequence in EcoCyc

Transcription of LuxS (interaction with micA,gshA)

http://gcat.davidson.edu/GcatWiki/images/c/cb/N654260305_1347432_6212.jpg

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